2019
Structural basis for the clamping and Ca2+ activation of SNARE-mediated fusion by synaptotagmin
Grushin K, Wang J, Coleman J, Rothman JE, Sindelar CV, Krishnakumar SS. Structural basis for the clamping and Ca2+ activation of SNARE-mediated fusion by synaptotagmin. Nature Communications 2019, 10: 2413. PMID: 31160571, PMCID: PMC6546687, DOI: 10.1038/s41467-019-10391-x.Peer-Reviewed Original ResearchConceptsCryo-electron microscopy structureActivation of SNAREsDependent membrane interactionsAnionic lipid headgroupsFusion clampActivator functionSNARE bundleSNARE proteinsMicroscopy structureC2B domainStructural basisSynaptotagmin-1SNAREpinsAliphatic loopsMembrane interactionsComplete assemblyLipid headgroupsLipid membranesNeurotransmitter releaseMembraneKey determinantSynaptotagminSyt1Calcium influxPartial insertionSynaptotagmin oligomers are necessary and can be sufficient to form a Ca2+‐sensitive fusion clamp
Ramakrishnan S, Bera M, Coleman J, Krishnakumar SS, Pincet F, Rothman JE. Synaptotagmin oligomers are necessary and can be sufficient to form a Ca2+‐sensitive fusion clamp. FEBS Letters 2019, 593: 154-162. PMID: 30570144, PMCID: PMC6349546, DOI: 10.1002/1873-3468.13317.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCryoelectron MicroscopyElectron Microscope TomographyMembrane FusionMutationProtein MultimerizationSNARE ProteinsSynaptic VesiclesSynaptotagmin I
2010
Protein Determinants of SNARE-Mediated Lipid Mixing
Ji H, Coleman J, Yang R, Melia TJ, Rothman JE, Tareste D. Protein Determinants of SNARE-Mediated Lipid Mixing. Biophysical Journal 2010, 99: 553-560. PMID: 20643074, PMCID: PMC2905075, DOI: 10.1016/j.bpj.2010.04.060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCryoelectron MicroscopyKineticsLipidsMembrane FusionMiceMolecular WeightProteolipidsRatsSNARE ProteinsSurface PropertiesConceptsSNARE proteinsN-ethylmaleimide-sensitive factor attachment protein receptorsSoluble N-ethylmaleimide-sensitive factor attachment protein receptorsSensitive factor attachment protein receptorsFactor attachment protein receptorsT-SNARE complexMembrane SNARE proteinsT-SNARE proteinsAttachment protein receptorsLipid mixingMembrane SNAREsProtein receptorsProtein determinantsReconstitution conditionsReconstitution protocolsSnareLiposome fusionProteinLiposome populationsSpecific activityLipidsOptimal lipidProteoliposomesPhysiologyRecent work