2018
High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis
Ramakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis. Langmuir 2018, 34: 5849-5859. PMID: 29694054, DOI: 10.1021/acs.langmuir.8b00116.Peer-Reviewed Original ResearchConceptsMembrane fusionFusion eventsSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsSNARE-dependent membrane fusionIndividual vesicle fusion eventsFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsT-SNARE proteinsSingle-vesicle fusionProtein receptor proteinsVesicle fusion eventsMobility of proteinsVesicle dockingContent releaseVesicle fusionHigh-throughput monitoringPlanar membranesReceptor proteinLipid mixingProteinLipid bilayersVesiclesCorrect reconstitutionMembraneAqueous compartment
2010
Protein Determinants of SNARE-Mediated Lipid Mixing
Ji H, Coleman J, Yang R, Melia TJ, Rothman JE, Tareste D. Protein Determinants of SNARE-Mediated Lipid Mixing. Biophysical Journal 2010, 99: 553-560. PMID: 20643074, PMCID: PMC2905075, DOI: 10.1016/j.bpj.2010.04.060.Peer-Reviewed Original ResearchConceptsSNARE proteinsN-ethylmaleimide-sensitive factor attachment protein receptorsSoluble N-ethylmaleimide-sensitive factor attachment protein receptorsSensitive factor attachment protein receptorsFactor attachment protein receptorsT-SNARE complexMembrane SNARE proteinsT-SNARE proteinsAttachment protein receptorsLipid mixingMembrane SNAREsProtein receptorsProtein determinantsReconstitution conditionsReconstitution protocolsSnareLiposome fusionProteinLiposome populationsSpecific activityLipidsOptimal lipidProteoliposomesPhysiologyRecent work