2024
Hepatic GDP-fucose transporter SLC35C1 attenuates cholestatic liver injury and inflammation by inducing CEACAM1 N153 fucosylation.
Zhang L, Xie P, Li M, Zhang X, Fei S, Zhao N, Li L, Xie Q, Xu Z, Tang W, Zhu G, Zhu Z, Xu Z, Li J, Zhang C, Boyer J, Chen W, Cai S, Pan Q, Chai J. Hepatic GDP-fucose transporter SLC35C1 attenuates cholestatic liver injury and inflammation by inducing CEACAM1 N153 fucosylation. Hepatology 2024 PMID: 38985995, DOI: 10.1097/hep.0000000000001003.Peer-Reviewed Original ResearchMouse model of cholestasisModel of cholestasisCholestatic liver injuryLiver injuryMouse modelCXCL2 expressionSolute carrier familyTandem mass spectrometry analysisPrimary mouse hepatocytesLiver-specific ablationMass spectrometry analysisProtein glycosylationSLC35C1Attenuate cholestatic liver injurySTAT3 signalingBile ductular proliferationBile duct ligationCarrier familyLevels of serumCholic acid feedingMolecular mechanismsIncreased liver necrosisMRNA transcriptsFucosylationCXCL2 mRNA expression
2022
SEMA7AR148W mutation promotes lipid accumulation and NAFLD progression via increased localization on the hepatocyte surface
Zhao N, Zhang X, Ding J, Pan Q, Zheng MH, Liu WY, Luo G, Qu J, Li M, Li L, Cheng Y, Peng Y, Xie Q, Wei Q, Li Q, Zou L, Ouyang X, Cai SY, Boyer JL, Chai J. SEMA7AR148W mutation promotes lipid accumulation and NAFLD progression via increased localization on the hepatocyte surface. JCI Insight 2022, 7: e154113. PMID: 35938531, PMCID: PMC9462498, DOI: 10.1172/jci.insight.154113.Peer-Reviewed Original ResearchConceptsIntegrin β1Lipid accumulationPrimary mouse hepatocytesProtein interactionsLipid droplet accumulationMouse liverFatty acid oxidationHeterozygous mutationsIntegrin β1 proteinPKC-α phosphorylationFA uptakeGenetic determinantsMouse peritoneal macrophagesCell membraneStrong genetic determinantsMutationsMouse hepatocytesDroplet accumulationΒ1 proteinCD36 expressionAcid oxidationPKCTriglyceride synthesisGenetic polymorphismsAccumulation
2000
Induction of murine hepatocyte death by membrane‐bound CD95 (Fas/APO‐1)‐ligand: Characterization of an in Vitro system
Schlosser S, Azzaroli F, Dao T, Hingorani R, Crispe I, Boyer J. Induction of murine hepatocyte death by membrane‐bound CD95 (Fas/APO‐1)‐ligand: Characterization of an in Vitro system. Hepatology 2000, 32: 779-785. PMID: 11003622, DOI: 10.1053/jhep.2000.18422.Peer-Reviewed Original ResearchConceptsMouse hepatocytesMembrane-bound CD95LNIH 3T3 fibroblastsFas/APOProtein synthesis inhibitorPrimary mouse hepatocytesDNA strand breaksCultured mouse hepatocytesNuclear condensationAnti-CD95 antibodiesCell deathNuclear fragmentationBiochemical studiesStrand breaksCD95 ligandVitro systemCell apoptosisExposure of hepatocytesApoptosisCD95Potential targetCD95LSynthesis inhibitorCytoplasmic blebsLiver cell apoptosis