2014
Coupling between endocytosis and sphingosine kinase 1 recruitment
Shen H, Giordano F, Wu Y, Chan J, Zhu C, Milosevic I, Wu X, Yao K, Chen B, Baumgart T, Sieburth D, De Camilli P. Coupling between endocytosis and sphingosine kinase 1 recruitment. Nature Cell Biology 2014, 16: 652-662. PMID: 24929359, PMCID: PMC4230894, DOI: 10.1038/ncb2987.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditisCell MembraneCells, CulturedChlorocebus aethiopsCholesterolCOS CellsEndocytosisFluorescent Antibody TechniqueHEK293 CellsHeLa CellsHumansMiceModels, MolecularMutationPhosphotransferases (Alcohol Group Acceptor)Protein BindingProtein Structure, TertiarySequence AnalysisConceptsSphingosine kinase 1Hydrophobic patchN-BAR proteinsEndocytic membrane trafficExo/endocytosisPlasma membrane resultsMembrane trafficEndocytic intermediatesFunction mutantsMembrane recruitmentEndocytic membranesCellular compartmentsIntercellular signalingRecycling defectsSphingosine phosphorylationKinase 1Genetic studiesFunctional linkTubular invaginationsSphingolipid metabolismLipid bilayersEndocytosisEnzyme surfaceMembrane resultsRecruitment
2013
Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors
Park RJ, Shen H, Liu L, Liu X, Ferguson SM, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. Journal Of Cell Science 2013, 126: 5305-5312. PMID: 24046449, PMCID: PMC3828596, DOI: 10.1242/jcs.138578.Peer-Reviewed Original ResearchConceptsDynamin 1Dynamin inhibitorTKO cellsPeripheral membrane rufflingDouble knockoutDynamin 3 geneClathrin-mediated endocytosisTriple-knockout cellsDynamin-dependent processFluid-phase endocytosisDyngo-4aMembrane fissionMembrane rufflingDKO cellsDynamin 2Knockout cellsLow-level expressionCell physiologyDynaminEndocytosisTarget effectsDKO fibroblastsGenesCellsKO fibroblasts
2012
Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane
Sousa LP, Lax I, Shen H, Ferguson SM, De Camilli P, Schlessinger J. Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 4419-4424. PMID: 22371560, PMCID: PMC3311323, DOI: 10.1073/pnas.1200164109.Peer-Reviewed Original ResearchConceptsEGFR endocytosisPlasma membraneDynamin-mediated endocytosisEndocytosis of EGFRRole of endocytosisLow-affinity EGFRsEGF receptor activationCell surface receptorsMAPK responseMAPK stimulationEGFR degradationAkt stimulationCell signalingDynaminEGFR activationEGFR autophosphorylationAkt activationEndocytosisCell surfaceSurface receptorsMouse fibroblastsAkt responseReceptor activationHigh EGF concentrationsActivation