2008
A Randomized Controlled Trial of Glucose versus Amylase Resistant Starch Hypo-Osmolar Oral Rehydration Solution for Adult Acute Dehydrating Diarrhea
Ramakrishna BS, Subramanian V, Mohan V, Sebastian BK, Young GP, Farthing MJ, Binder HJ. A Randomized Controlled Trial of Glucose versus Amylase Resistant Starch Hypo-Osmolar Oral Rehydration Solution for Adult Acute Dehydrating Diarrhea. PLOS ONE 2008, 3: e1587. PMID: 18270575, PMCID: PMC2217593, DOI: 10.1371/journal.pone.0001587.Peer-Reviewed Original ResearchConceptsHypo-osmolar ORSOral rehydration solutionCholera-like diarrheaDiarrhea durationDehydrating diarrheaFecal weightRehydration solutionDuration of diarrheaTertiary referral hospitalSevere dehydrating diarrheaSevere watery diarrheaAmylase-resistant starchAcute dehydrating diarrheaSimilar significant differencesORS intakeORS therapyControlled TrialsIntravenous therapyReferral hospitalAppropriate antibioticsRehydration therapyWatery diarrheaStool weightSubgroup analysisClinical trials
2006
Amylase‐Resistant Starch as Adjunct to Oral Rehydration Therapy in Children with Diarrhea
Raghupathy P, Ramakrishna BS, Oommen SP, Ahmed MS, Priyaa G, Dziura J, Young GP, Binder HJ. Amylase‐Resistant Starch as Adjunct to Oral Rehydration Therapy in Children with Diarrhea. Journal Of Pediatric Gastroenterology And Nutrition 2006, 42: 362-368. PMID: 16641573, DOI: 10.1097/01.mpg.0000214163.83316.41.Peer-Reviewed Original ResearchConceptsOral rehydration solutionAmylase-resistant starchGlucose-ORSDuration of diarrheaStool weightAcute diarrheaStandard treatmentFluid absorptionG-ORS groupMean stool weightIntravenous fluid therapyAcute watery diarrheaOral rehydration therapySeverity of diarrheaTreatment of diarrheaShort-chain fatty acidsColonic sodiumEvaluable dataRehydration therapyTreatment failureFluid therapyWatery diarrheaRehydration solutionIntestinal sodiumDiarrhea
2004
Zinc inhibits cAMP-stimulated Cl secretion via basolateral K-channel blockade in rat ileum
Hoque KM, Rajendran VM, Binder HJ. Zinc inhibits cAMP-stimulated Cl secretion via basolateral K-channel blockade in rat ileum. AJP Gastrointestinal And Liver Physiology 2004, 288: g956-g963. PMID: 15618279, DOI: 10.1152/ajpgi.00441.2004.Peer-Reviewed Original ResearchMeSH Keywords8-Bromo Cyclic Adenosine MonophosphateAnimalsBariumCarbacholChloridesColforsinCyclic AMPDiarrheaEnterocytesIleumMaleMembrane GlycoproteinsMonosaccharide Transport ProteinsPotassium ChannelsRatsRats, Sprague-DawleySodiumSodium-Glucose Transporter 1Sodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Time FactorsZincConceptsCl(-) secretionRat ileumNa absorptionK channel blockadeBumetanide-sensitive 86Rb uptakeK channelsPresence of ouabainHalf maximal inhibitory concentrationNa-K-ATPaseAcute diarrheaStool outputCAMP-induced Cl- secretionMucosal additionMuscle layerAnion secretionLucite chamberCotransport activitySecretionClamp methodInhibitory concentrationDiarrheaCyclic monophosphateIleumEfflux experimentsSerosalThree distinct mechanisms of HCO3− secretion in rat distal colon
Vidyasagar S, Rajendran VM, Binder HJ. Three distinct mechanisms of HCO3− secretion in rat distal colon. American Journal Of Physiology - Cell Physiology 2004, 287: c612-c621. PMID: 15308466, DOI: 10.1152/ajpcell.00474.2003.Peer-Reviewed Original ResearchConceptsRat distal colonShort-chain fatty acidsColonic mucosaDistal colonRat distal colonic mucosaDistal colonic mucosaCl- channel blockerSCFA/Channel blockersSerosal layerCrypt cellsCFTR blockerMicroM DIDSApical membrane anion channelApical membraneSecretionDependent secretionColonLumen ClSecretory mechanismMammalian colonBlockersMucosaStat techniqueDIDS
2003
Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon
Krishnan S, Rajendran VM, Binder HJ. Apical NHE isoforms differentially regulate butyrate-stimulated Na absorption in rat distal colon. American Journal Of Physiology - Cell Physiology 2003, 285: c1246-c1254. PMID: 12878493, DOI: 10.1152/ajpcell.00598.2002.Peer-Reviewed Original Research
2001
Na+-dependent fluid absorption in intact perfused rat colonic crypts
Geibel J, Rajendran V, Binder H. Na+-dependent fluid absorption in intact perfused rat colonic crypts. Gastroenterology 2001, 120: 144-150. PMID: 11208723, DOI: 10.1053/gast.2001.20890.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesChloridesColonGanglionic StimulantsGlutamatesIn Vitro TechniquesIntestinal AbsorptionInulinIsotonic SolutionsMalePerfusionQuaternary Ammonium CompoundsRatsRats, Sprague-DawleyRinger's SolutionSodiumSodium-Hydrogen ExchangersTritiumConceptsNet fluid absorptionRat colonic cryptsCl- channel blockerFluid absorptionNeurohumoral stimulationChannel blockersColonic cryptsLumen ClAbsorptive processesInhibitor of NaCl(-) secretionAbstractTextCrypt cellsExchange inhibitorMicroperfusion techniqueFluid secretionL DIDSAmiloride analoguesMammalian colonSecretionAnion exchange inhibitorCryptsSignificant increaseAIMSSecretory processMorphology of Isolated Colonic Crypts
Robert M, Singh S, Ikuma M, Jain D, Ardito T, Binder H. Morphology of Isolated Colonic Crypts. Cells Tissues Organs 2001, 168: 246-251. PMID: 11275691, DOI: 10.1159/000047841.Peer-Reviewed Original Research
2000
Basolateral K-Cl Cotransporter Regulates Colonic Potassium Absorption in Potassium Depletion*
Sangan P, Brill S, Sangan S, Forbush B, Binder H. Basolateral K-Cl Cotransporter Regulates Colonic Potassium Absorption in Potassium Depletion*. Journal Of Biological Chemistry 2000, 275: 30813-30816. PMID: 10878016, DOI: 10.1074/jbc.m003931200.Peer-Reviewed Original ResearchConceptsK-Cl cotransporterMembrane HK-Cl cotransportActive potassium absorptionProtein expressionBasolateral membranePotassium absorptionK-ATPaseNorthern blot analysisKCC1 mRNACoordinated regulationAlpha-subunit mRNABeta-subunit mRNAMRNA abundanceBlot analysisMRNAExpressionProteinMembraneCotransporterDepletionRat distal colonCDNAKCC1Amylase-Resistant Starch plus Oral Rehydration Solution for Cholera
Ramakrishna BS, Venkataraman S, Srinivasan P, Dash P, Young G, Binder H. Amylase-Resistant Starch plus Oral Rehydration Solution for Cholera. New England Journal Of Medicine 2000, 342: 308-313. PMID: 10655529, DOI: 10.1056/nejm200002033420502.Peer-Reviewed Original ResearchConceptsOral rehydration solutionDuration of diarrheaOral rehydration therapyStandard therapyAmylase-resistant starchRehydration solutionFecal weightRehydration therapyStandard oral rehydration therapyMean fecal weightResistant starch groupStandard therapy groupPrimary end pointShort-chain fatty acidsMean durationFecal excretionDiarrheaTherapySodium absorptionEnd pointFluid lossLength of timeCholeraFatty acidsDuration
1999
Colonic H-K-ATPase β-subunit: identification in apical membranes and regulation by dietary K depletion
Sangan P, Kolla S, Rajendran V, Kashgarian M, Binder H. Colonic H-K-ATPase β-subunit: identification in apical membranes and regulation by dietary K depletion. American Journal Of Physiology 1999, 276: c350-c360. PMID: 9950762, DOI: 10.1152/ajpcell.1999.276.2.c350.Peer-Reviewed Original ResearchDifferential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon
Ikuma M, Kashgarian M, Binder H, Rajendran V. Differential regulation of NHE isoforms by sodium depletion in proximal and distal segments of rat colon. American Journal Of Physiology 1999, 276: g539-g549. PMID: 9950829, DOI: 10.1152/ajpgi.1999.276.2.g539.Peer-Reviewed Original ResearchConceptsDietary sodium depletionSodium depletionDistal colonRat colonProtein expressionMRNA abundanceBlot analysisDose-dependent inhibitorApical membrane vesiclesElectroneutral NaCl absorptionWestern blot analysisDifferential regulationNormal ratsProximal colonHOE 694Multiple diverse effectsColonDistal segmentsNaCl absorptionNHE isoformsExchange activityExchanger isoformsNorthern blot analysisDifferential effectsPretranslational levelRole of Cl channels in Cl-dependent Na/H exchange
Rajendran V, Geibel J, Binder H. Role of Cl channels in Cl-dependent Na/H exchange. American Journal Of Physiology 1999, 276: g73-g78. PMID: 9886981, DOI: 10.1152/ajpgi.1999.276.1.g73.Peer-Reviewed Original ResearchConceptsNa/H exchangeCl- channel blockerChannel blockersMicroM DIDSNa/H exchange activityCystic fibrosis transmembrane conductance regulatorIntracellular pH recoveryRat distal colonCl- channelsH exchange activityDistal colonCrypt lumenCrypt cellsAcid loadExchange inhibitorApical membranePH recoveryCl(-) dependenceAnion exchange inhibitorCl- channel activityChannel activityBlockersH exchangePolyclonal antibodiesCl- conductance
1998
Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells
Ikuma M, Binder H, Geibel J. Role of Apical H-K Exchange and Basolateral K Channel in the Regulation of Intracellular pH in Rat Distal Colon Crypt Cells. The Journal Of Membrane Biology 1998, 166: 205-212. PMID: 9843594, DOI: 10.1007/s002329900462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntiportersBariumCell PolarityColonEpithelial CellsH(+)-K(+)-Exchanging ATPaseHomeostasisHydrogen-Ion ConcentrationIntestinal MucosaIntracellular FluidIon TransportMaleOuabainPotassiumPotassium ChannelsPotassium-Hydrogen AntiportersRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseDifferential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells
Rajendran V, Singh S, Geibel J, Binder H. Differential localization of colonic H+-K+-ATPase isoforms in surface and crypt cells. American Journal Of Physiology 1998, 274: g424-g429. PMID: 9486199, DOI: 10.1152/ajpgi.1998.274.2.g424.Peer-Reviewed Original ResearchConceptsCrypt cellsColonic cryptsRat distal colonSurface cellsSurface epithelial cellsATPase isoformsOuabain-insensitive componentApical membraneDistal colonAcid loadDependent intracellularEpithelial cellsATPase activityCryptsDifferential localizationOuabainATPase alpha subunitCellsAlpha subunitCompelling evidenceIsoformsColonActivityRecovery
1997
Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion
Sangan P, Rajendran V, Mann A, Kashgarian M, Binder H. Regulation of colonic H-K-ATPase in large intestine and kidney by dietary Na depletion and dietary K depletion. American Journal Of Physiology 1997, 272: c685-c696. PMID: 9124313, DOI: 10.1152/ajpcell.1997.272.2.c685.Peer-Reviewed Original ResearchConceptsDietary K depletionDistal colonNA depletionK-ATPase activityProximal colonLarge intestineRat distal colonActive K absorptionK-ATPaseRenal collecting ductsDietary NaK-ATPase expressionK-ATPase isoformsK depletionRat colonCollecting ductsOuter medullaColonProtein expressionProtein levelsKidneyPolyclonal antibodiesIntestineDuctMRNA
1995
Fluid absorption in isolated perfused colonic crypts.
Singh S, Binder H, Boron W, Geibel J. Fluid absorption in isolated perfused colonic crypts. Journal Of Clinical Investigation 1995, 96: 2373-2379. PMID: 7593625, PMCID: PMC185889, DOI: 10.1172/jci118294.Peer-Reviewed Original ResearchConceptsCrypt cellsNM vasoactive intestinal peptideFluid absorptionLarge intestinal functionColonic cryptsVasoactive intestinal peptideLamina propria cellsNet fluid secretionRat distal colonNet fluid absorptionDibutyryl cyclic AMPIntestinal peptideDistal colonIntestinal functionLamina propriaPathophysiological paradigmCrypt preparationMM acetylcholineBasal stateNeurohumoral agonistsFluid secretionCellular elementsEpithelial cellsIntestinal cryptsCryptsDiagnostic utility of contrast echocardiography and lung perfusion scan in patients with hepatopulmonary syndrome
Abrams G, Jaffe C, Hoffer P, Binder H, Fallon M. Diagnostic utility of contrast echocardiography and lung perfusion scan in patients with hepatopulmonary syndrome. Gastroenterology 1995, 109: 1283-1288. PMID: 7557096, DOI: 10.1016/0016-5085(95)90589-8.Peer-Reviewed Original ResearchConceptsLung perfusion scanHepatopulmonary syndromeIntrapulmonary vascular dilatationEchocardiogram resultsPerfusion scanContrast echocardiographyVascular dilatationDiagnosis of HPSBiopsy-proven cirrhosisConcurrent cardiopulmonary diseaseIntrinsic lung diseaseLung scan resultsPositive contrast echocardiogramChest radiograph abnormalitiesArterial blood gasesGas exchange abnormalitiesScan resultsUseful screening testIntrapulmonary vasodilatationPositive echocardiogramRadiograph abnormalitiesConsecutive outpatientsCardiopulmonary diseaseLung diseasePositive lungChloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*
Rajendran V, Geibel J, Binder H. Chloride-dependent Na-H Exchange A NOVEL MECHANISM OF SODIUM TRANSPORT IN COLONIC CRYPTS*. Journal Of Biological Chemistry 1995, 270: 11051-11054. PMID: 7744735, DOI: 10.1074/jbc.270.19.11051.Peer-Reviewed Original ResearchConceptsNa-H exchangeColonic crypt cellsCrypt cellsAcid loadRat distal colonApical membrane vesiclesNovel transport processRate of intracellularDistal colonMM amilorideSodium transportApical membraneGradient stimulationColonic cryptsAmiloride inhibitionSodium movementNovel mechanismOutward gradientCompelling evidenceCellsSodiumUptakePrior studiesColonRecovery
1993
Mechanism of leukotriene D4 stimulation of Cl- secretion in rat distal colon in vitro
Hyun C, Binder H. Mechanism of leukotriene D4 stimulation of Cl- secretion in rat distal colon in vitro. American Journal Of Physiology 1993, 265: g467-g473. PMID: 8214069, DOI: 10.1152/ajpgi.1993.265.3.g467.Peer-Reviewed Original ResearchConceptsEffect of LTD4Inflammatory bowel diseaseLeukotriene D4Specific LTD4 receptor antagonistLamina propria cellsMediators of inflammationLTD4 receptor antagonistRat distal colonRat colonic mucosaElectrogenic Cl- secretionLTD4 effectsVoltage-clamp conditionsBowel diseaseSerotonergic receptorsReceptor antagonistCyclooxygenase metabolitesDistal colonColonic mucosaCyclooxygenase inhibitorSerosal additionCotransport inhibitorMaximal responseCl(-) secretionLipoxygenase metabolitesArachidonic acid
1992
Mucosal ouabain and Na+ inhibit active Rb+(K+) absorption in normal and sodium-depleted rat distal colon
Pandiyan V, Rajendran V, Binder H. Mucosal ouabain and Na+ inhibit active Rb+(K+) absorption in normal and sodium-depleted rat distal colon. Gastroenterology 1992, 102: 1846-1853. PMID: 1587404, DOI: 10.1016/0016-5085(92)90304-h.Peer-Reviewed Original Research