2016
Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins
Jensen KH, Brandt SS, Shigematsu H, Sigworth FJ. Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins. Journal Of Structural Biology 2016, 194: 49-60. PMID: 26835990, PMCID: PMC4866491, DOI: 10.1016/j.jsb.2016.01.012.Peer-Reviewed Original Research
1996
Impaired slow inactivation in mutant sodium channels
Cummins T, Sigworth F. Impaired slow inactivation in mutant sodium channels. Biophysical Journal 1996, 71: 227-236. PMID: 8804606, PMCID: PMC1233474, DOI: 10.1016/s0006-3495(96)79219-6.Peer-Reviewed Original ResearchConceptsRat skeletal muscle channelsSodium currentProlonged depolarizationSlow inactivationPersistent sodium currentMaximal sodium currentSkeletal muscle channelsMuscle paralysisSkeletal muscle fibersMutant sodium channelsSustained currentHyperkalemic periodic paralysisM mutationPeriodic paralysisSodium channelsMuscle channelsMuscle fibersParalysisFast inactivationWild-type channelsMutant channelsDepolarization
1994
Oxygen deprivation activates an ATP-inhibitable K+ channel in substantia nigra neurons
Jiang C, Sigworth F, Haddad G. Oxygen deprivation activates an ATP-inhibitable K+ channel in substantia nigra neurons. Journal Of Neuroscience 1994, 14: 5590-5602. PMID: 8083755, PMCID: PMC6577106, DOI: 10.1523/jneurosci.14-09-05590.1994.Peer-Reviewed Original ResearchConceptsSubstantia nigra neuronsRat substantia nigra neuronsEffects of hypoxiaGlibenclamide binding sitesEffect of ATPCell-attached patchesNeuronal survivalKATP channelsResult of Ca2Different subtypesOutward currentsNeuronal structuresFree Ca2HypoxiaMetabolic stressNeuronsDeleterious changesSingle-channel currentsChannel activityO2 deprivationInternal ATPOxygen deprivationActivationHigh Ca2Membrane potential
1989
Primary structure and functional expression of a mammalian skeletal muscle sodium channel
Trimmer J, Cooperman S, Tomiko S, Zhou J, Crean S, Boyle M, Kalen R, Sheng Z, Barchi R, Sigworth F, Goodman R, Agnew W, Mandel G. Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 1989, 3: 33-49. PMID: 2559760, DOI: 10.1016/0896-6273(89)90113-x.Peer-Reviewed Original ResearchConceptsAlpha subunitHomologous domains IAmino acid segmentSodium channelsSkeletal muscle sodium channelsNorthern blot analysisSkeletal muscleMuscle sodium channelsVoltage-sensitive sodium channelsPrimary structureFunctional expressionDomain IAcid segmentNative channelsSynthetic RNAXenopus oocytesRat muscle fibersBlot analysisRat skeletal muscleEel electroplaxRat brainSubunitsMuscle fibersMuscleCDNA
1986
Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor
Sigworth F. Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor. Biophysical Journal 1986, 49: 1041-1046. PMID: 2423148, PMCID: PMC1329684, DOI: 10.1016/s0006-3495(86)83732-8.Peer-Reviewed Original Research
1981
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches
Hamill O, Marty A, Neher E, Sakmann B, Sigworth F. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Archiv - European Journal Of Physiology 1981, 391: 85-100. PMID: 6270629, DOI: 10.1007/bf00656997.Peer-Reviewed Original Research
1980
Single Na+ channel currents observed in cultured rat muscle cells
Sigworth F, Neher E. Single Na+ channel currents observed in cultured rat muscle cells. Nature 1980, 287: 447-449. PMID: 6253802, DOI: 10.1038/287447a0.Peer-Reviewed Original Research