2019
A cell-penetrating peptide blocks Toll-like receptor-mediated downstream signaling and ameliorates autoimmune and inflammatory diseases in mice
Kwon HK, Patra MC, Shin HJ, Gui X, Achek A, Panneerselvam S, Kim DJ, Song SJ, Hong R, Kim KS, Kim YG, Lee FY, Hahm DH, Lee SH, Choi S. A cell-penetrating peptide blocks Toll-like receptor-mediated downstream signaling and ameliorates autoimmune and inflammatory diseases in mice. Experimental & Molecular Medicine 2019, 51: 1-19. PMID: 31028244, PMCID: PMC6486608, DOI: 10.1038/s12276-019-0244-0.Peer-Reviewed Original ResearchConceptsToll-like receptorsCollagen-induced arthritisInflammatory diseasesRodent modelsOverexpression of TLRsMultiple Toll-like receptorsAutoimmune/inflammatory diseasesSecretion of lipopolysaccharideDamage-associated molecular patternsInflammatory signaling cascadesIL-1β secretionNumerous inflammatory diseasesRheumatoid arthritisProinflammatory cytokinesLiver damageTLR4 TIR domainImmune responseAnimal studiesTherapeutic potentialToll/interleukinMolecular patternsMRNA levelsDiseaseArthritisSelective inhibitor
2017
Tendon stem/progenitor cells regulate inflammation in tendon healing via JNK and STAT3 signaling
Tarafder S, Chen E, Jun Y, Kao K, Sim KH, Back J, Lee FY, Lee CH. Tendon stem/progenitor cells regulate inflammation in tendon healing via JNK and STAT3 signaling. The FASEB Journal 2017, 31: 3991-3998. PMID: 28533328, PMCID: PMC5572690, DOI: 10.1096/fj.201700071r.Peer-Reviewed Original ResearchConceptsConnective tissue growth factorTendon stem/progenitor cellsIL-10 expressionStem/progenitor cellsIL-10TIMP-3 expressionTendon healingIL-1βProgenitor cellsTendon cellsElevated IL-10 expressionAnti-inflammatory IL-10Number of iNOSRat patellar tendonAnti-inflammatory roleAcute tendon injuryIL-6 expressionRegulation of inflammationTissue growth factorMMP-3 expressionNumber of CD146Improved tendon healingDifferent anatomic locationsWestern blot analysisPromising regenerative potential
2015
Harnessing endogenous stem/progenitor cells for tendon regeneration
Lee CH, Lee FY, Tarafder S, Kao K, Jun Y, Yang G, Mao JJ. Harnessing endogenous stem/progenitor cells for tendon regeneration. Journal Of Clinical Investigation 2015, 125: 2690-2701. PMID: 26053662, PMCID: PMC4563693, DOI: 10.1172/jci81589.Peer-Reviewed Original ResearchMeSH KeywordsAdult Stem CellsAnimalsCD146 AntigenCell DifferentiationCell ProliferationColony-Forming Units AssayConnective Tissue Growth FactorFocal Adhesion Kinase 1MAP Kinase Signaling SystemMultipotent Stem CellsRatsRats, Sprague-DawleyRegenerationTendon InjuriesTendonsTissue EngineeringWound HealingConceptsStem/progenitor cellsEndogenous stem/progenitor cellsProgenitor cellsCell transplantationTendon regenerationStem cell markers CD146Stem cell-based strategiesFAK/ERK1/2Cell-based strategiesRat modelTendon healingFunctional restorationNormal levelsMultilineage differentiation abilityClonogenic capacityCD146Tendon cellsSiRNA knockdownTransplantationVivo manipulation
2014
Effects of biomechanical forces on signaling in the cortical collecting duct (CCD)
Carrisoza-Gaytan R, Liu Y, Flores D, Else C, Lee HG, Rhodes G, Sandoval RM, Kleyman TR, Lee FY, Molitoris B, Satlin LM, Rohatgi R. Effects of biomechanical forces on signaling in the cortical collecting duct (CCD). American Journal Of Physiology. Renal Physiology 2014, 307: f195-f204. PMID: 24872319, PMCID: PMC4152160, DOI: 10.1152/ajprenal.00634.2013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutocrine CommunicationCell LineDinoprostoneEnzyme ActivationExtracellular Signal-Regulated MAP KinasesFurosemideInjectionsIon TransportKidney CortexKidney Tubules, CollectingMechanotransduction, CellularMiceMicroscopy, Fluorescence, MultiphotonP38 Mitogen-Activated Protein KinasesParacrine CommunicationPhosphorylationPotassiumRatsRats, Sprague-DawleySodium ChlorideStress, MechanicalTime FactorsConceptsTubular fluid flow rateP-p38Biomechanical forcesCircumferential stretchIntravital microscopic approachP-ERK expressionFurosemide injectionPGE2 releaseIntracellular MAPKK secretionCollagen type IVCell culture modelTubular diameterEx vivoPGE2Fluid shear stressUnstretched controlsCCD cellsType IVP38 activationCulture modelMAPK signalingVivoPhysiological forcesCation transport
2013
Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
Amorosa L, Lee C, Aydemir, Nizami S, Hsu A, Patel N, Gardner T, Navalgund A, Kim DG, Park S, Mao J, Lee F. Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study. International Journal Of Nanomedicine 2013, Volume 8: 1637-1643. PMID: 23637532, PMCID: PMC3639117, DOI: 10.2147/ijn.s42855.Peer-Reviewed Original ResearchConceptsAddition of hMSCsPolymer-based scaffoldsLoad-bearing characteristicsPhysiologic cyclic loadingPhase angleCritical-sized segmental defectsScaffold alone groupViscous stiffnessCyclic loadingMechanical propertiesFemoral defect modelSized segmental defectsRat femoral defect modelHuman mesenchymal stem cellsMechanical simulationsScaffold groupDifferent biomechanical characteristicsEnhanced bone formationHigh phase anglesDefect modelBiomechanical characteristicsExperimental studyHost boneStiffnessDefect repair
2009
Improving bone density at the rotator cuff footprint increases supraspinatus tendon failure stress in a rat model
Cadet ER, Vorys GC, Rahman R, Park S, Gardner TR, Lee FY, Levine WN, Bigliani LU, Ahmad CS. Improving bone density at the rotator cuff footprint increases supraspinatus tendon failure stress in a rat model. Journal Of Orthopaedic Research® 2009, 28: 308-314. PMID: 19743506, DOI: 10.1002/jor.20972.Peer-Reviewed Original ResearchConceptsBone densityRotator cuff footprintOVX groupRat modelSupraspinatus tendonCuff footprintQuantitative micro-computed tomographyMonths of ageBisphosphonate treatmentOVX ratsBilateral ovariectomyProximal humerusDawley ratsAdditional ratsOVXRatsTendonOvariectomyMicro-computed tomographyTreatmentGroupBisphosphonatesSpragueHumerusMonthsZoledronate reduces unwanted bone resorption in intercalary bone allografts
Seo SW, Cho SK, Storer SK, Lee FY. Zoledronate reduces unwanted bone resorption in intercalary bone allografts. International Orthopaedics 2009, 34: 599-603. PMID: 19343345, PMCID: PMC2903128, DOI: 10.1007/s00264-009-0748-7.Peer-Reviewed Original ResearchConceptsAllograft resorptionIntercalary allograftsBone resorptionBone allograftControl groupZoledronate solutionIntercalary bone allograftsBone formationBone mineral densitySynthetic pyrophosphate analoguesHigh-dose bisphosphonatesPharmacological modificationGraft incorporationMineral densityBone densityAllograftsIdeal doseResorptionAnimal trialsHistological analysisBisphosphonatesSegmental defectsPyrophosphate analogsExperimental groupRat femur
2008
Enhancement of Periprosthetic Bone Quality with Topical Hydroxyapatite-Bisphosphonate Composite
Suratwala SJ, Cho SK, van Raalte JJ, Park SH, Seo SW, Chang SS, Gardner TR, Lee FY. Enhancement of Periprosthetic Bone Quality with Topical Hydroxyapatite-Bisphosphonate Composite. Journal Of Bone And Joint Surgery 2008, 90: 2189-2196. PMID: 18829917, PMCID: PMC2657477, DOI: 10.2106/jbjs.g.00409.Peer-Reviewed Original ResearchConceptsPeriprosthetic bone qualityPeriprosthetic bone massSix-month groupBone qualitySix weeksOsseous integrationBone massParticle-induced periprosthetic osteolysisImplant looseningDual X-ray absorptiometryInflammatory bone lossX-ray absorptiometryMean bone areaUltra-high molecular weight polyethyleneBone lossSix-week groupUltra-high molecular weight polyethylene particlesIntramedullary nailFemoral canalMolecular weight polyethyleneControl groupPeriprosthetic osteolysisBone areaPeak pullout forceIntramedullary implants
2000
Experimental model for allograft incorporation and allograft fracture repair
Lee F, Hazan E, Gebhardt M, Mankin H. Experimental model for allograft incorporation and allograft fracture repair. Journal Of Orthopaedic Research® 2000, 18: 303-306. PMID: 10815832, DOI: 10.1002/jor.1100180219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFracture Fixation, IntramedullaryFracture HealingOsteotomyRatsRats, Inbred LewRats, Sprague-DawleyTransplantation, HomologousConceptsAllograft incorporationFracture repairHost-donor interfaceSprague-Dawley ratsSkeletal defectsAdult LewisImmediate ambulationIntercalary allograftsRat modelHistological examinationOsteotomy healingKirschner wiresMuscle layerOsteotomy siteTransverse osteotomyExperimental modelHealing processAllograftsRepair processRepairAmbulation
1998
Micromechanical Properties of Epiphyseal Trabecular Bone and Primary Spongiosa Around the Physis: An In Situ Nanoindentation Study
Lee F, Rho J, Harten R, Parsons J, Behrens F. Micromechanical Properties of Epiphyseal Trabecular Bone and Primary Spongiosa Around the Physis: An In Situ Nanoindentation Study. Journal Of Pediatric Orthopaedics 1998, 18: 582-585. PMID: 9746404, DOI: 10.1097/00004694-199809000-00004.Peer-Reviewed Original ResearchProgrammed removal of chondrocytes during endochondral fracture healing
Lee F, Choi Y, Behrens F, DeFouw D, Einhorn T. Programmed removal of chondrocytes during endochondral fracture healing. Journal Of Orthopaedic Research® 1998, 16: 144-150. PMID: 9565087, DOI: 10.1002/jor.1100160124.Peer-Reviewed Original Research
1997
Regional and temporal changes in the acoustic properties of fracture callus in secondary bone healing
Harten R, Lee F, Zimmerman M, Hurowitz E, Arakal R, Behrens F. Regional and temporal changes in the acoustic properties of fracture callus in secondary bone healing. Journal Of Orthopaedic Research® 1997, 15: 570-576. PMID: 9379267, DOI: 10.1002/jor.1100150413.Peer-Reviewed Original ResearchMeSH KeywordsAcoustic Impedance TestsAnimalsBiomechanical PhenomenaBony CallusFemoral FracturesFracture HealingMaleMicroscopyRatsRats, Sprague-DawleyTime FactorsConceptsScanning acoustic microscopeMechanical propertiesAcoustic microscopyAcoustic microscopeMaterial propertiesAcoustic impedance mapsConventional testing methodsAcoustic propertiesImpedanceSecondary bone healingImpedance mapsTesting methodsPropertiesAccurate determinationBone healingPopular choiceFracturesLinear relationshipFracture healingDevicesPolymethylmethacrylateMicroscopeMean impedanceMaterials