2016
Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells
Tiwari M, Adaku N, Peart N, Rogers FA. Triplex structures induce DNA double strand breaks via replication fork collapse in NER deficient cells. Nucleic Acids Research 2016, 44: 7742-7754. PMID: 27298253, PMCID: PMC5027492, DOI: 10.1093/nar/gkw515.Peer-Reviewed Original ResearchConceptsDouble-strand breaksReplication fork collapseFork collapseGenomic instabilityReplication-associated double-strand breaksDNA replication fork progressionNucleotide excision repair proteinsTriplex structureRobust DNA damage responseDNA double-strand breaksReplication fork progressionReplication fork stabilityStrand breaksReplication fork integrityXPC-deficient cellsDNA damage responseMirror repeat sequencesExcision repair proteinsMore DSBsNER-deficient cellsDamage recognition proteinsGenomic integrityDual phosphorylationFork stabilityFork integrity
2013
XPD-dependent activation of apoptosis in response to triplex-induced DNA damage
Tiwari M, Rogers FA. XPD-dependent activation of apoptosis in response to triplex-induced DNA damage. Nucleic Acids Research 2013, 41: 8979-8994. PMID: 23913414, PMCID: PMC3799437, DOI: 10.1093/nar/gkt670.Peer-Reviewed Original ResearchConceptsDouble-strand breaksStrand breaksGenomic integrityPro-apoptotic pathwaysHuman genomeTyrosine 142DNA repairDNA sequencesGenomic instabilityDNA strand breaksNoncanonical structuresCancer initiationDNA damageDamage sitesΓH2AX fociHelical distortionApoptosisTriplex structureCentral rolePathwayXPDGenomeTriplexesPhosphorylationΓH2AX
2011
Targeted Disruption of the CCR5 Gene in Human Hematopoietic Stem Cells Stimulated by Peptide Nucleic Acids
Schleifman EB, Bindra R, Leif J, del Campo J, Rogers FA, Uchil P, Kutsch O, Shultz LD, Kumar P, Greiner DL, Glazer PM. Targeted Disruption of the CCR5 Gene in Human Hematopoietic Stem Cells Stimulated by Peptide Nucleic Acids. Cell Chemical Biology 2011, 18: 1189-1198. PMID: 21944757, PMCID: PMC3183429, DOI: 10.1016/j.chembiol.2011.07.010.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsHIV-1CCR5 geneHIV-1-infected individualsHIV-1 infectionGene modificationHIV-1 entryCCR5-Delta32 mutationImmune system functionStem cellsCCR5 knockoutMonths posttransplantationChemokine receptorsHuman hematopoietic stem cellsTherapeutic strategiesSubsequent engraftmentGenome modificationProtein levelsHuman cellsTargeted disruptionCCR5Peptide nucleic acidInfectionNucleic acidsCellsTargeted Gene Modification of Hematopoietic Progenitor Cells in Mice Following Systemic Administration of a PNA-peptide Conjugate
Rogers FA, Lin SS, Hegan DC, Krause DS, Glazer PM. Targeted Gene Modification of Hematopoietic Progenitor Cells in Mice Following Systemic Administration of a PNA-peptide Conjugate. Molecular Therapy 2011, 20: 109-118. PMID: 21829173, PMCID: PMC3255600, DOI: 10.1038/mt.2011.163.Peer-Reviewed Original ResearchConceptsGene modificationGene therapyHematopoietic stem cell gene therapyStem cell gene therapyGenomic modificationsVivo gene therapyCell gene therapyTargeted gene modificationVivo gene modificationHematopoietic progenitor cellsPeptide nucleic acidSystemic administrationBone marrowGene-targeting strategiesProgenitor cellsPrimary recipient miceStem cell mobilizationEx vivo manipulationSickle cell anemiaLymphoid cell lineagesDonor miceRecipient miceHematologic disordersInvasive alternativeCell mobilization
2009
Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids
Lonkar P, Kim KH, Kuan JY, Chin JY, Rogers FA, Knauert MP, Kole R, Nielsen PE, Glazer PM. Targeted correction of a thalassemia-associated β-globin mutation induced by pseudo-complementary peptide nucleic acids. Nucleic Acids Research 2009, 37: 3635-3644. PMID: 19364810, PMCID: PMC2699504, DOI: 10.1093/nar/gkp217.Peer-Reviewed Original ResearchConceptsBeta-globin geneNucleotide excision repair factorsAltered helical structureExcision repair factorsSingle base-pair modificationTriplex-forming peptide nucleic acidsPseudo-complementary peptide nucleic acidsDisease-related genesDonor DNA fragmentsComplementary DNA sequenceNucleic acidsProper splicingRepair factorsSite-specific bindingMammalian cellsSite-specific modificationDNA repairDNA sequencesGene targetingSecond intronDNA fragmentsHuman cellsTriple helix formationGene correctionHuman fibroblast cells
2004
Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides
Rogers FA, Manoharan M, Rabinovitch P, Ward DC, Glazer PM. Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides. Nucleic Acids Research 2004, 32: 6595-6604. PMID: 15602001, PMCID: PMC545466, DOI: 10.1093/nar/gkh998.Peer-Reviewed Original ResearchConceptsMutation frequencyGene-targeted mutagenesisHomeodomain of AntennapediaDNA-binding moleculesChromosomal genesThird helixTarget genesChromosomal DNABiological activityAntpCell-penetrating peptidesGene expressionControl genesTransport peptideCellular uptakeAntigene compoundsGenesConfocal microscopyIntranuclear deliveryTFODose-responsive mannerHomeodomainAntennapediaOligonucleotideMutagenesis