2020
Deep learning-based cross-classifications reveal conserved spatial behaviors within tumor histological images
Noorbakhsh J, Farahmand S, Foroughi pour A, Namburi S, Caruana D, Rimm D, Soltanieh-ha M, Zarringhalam K, Chuang JH. Deep learning-based cross-classifications reveal conserved spatial behaviors within tumor histological images. Nature Communications 2020, 11: 6367. PMID: 33311458, PMCID: PMC7733499, DOI: 10.1038/s41467-020-20030-5.Peer-Reviewed Original ResearchConceptsConvolutional neural networkWhole slide imagesPower of CNNsNormal convolutional neural networkImage data miningColon cancer imagesData miningCNN accuracyCancer imagesNeural networkHistopathological imagesManual inspectionSlide imagesData typesClassifier comparisonSignificant accuracyHistological imagesImage analysisSpatial similarityImagesClassifier pairsClassificationMutation classificationAccuracyMining
2014
Automated Objective Determination of Percentage of Malignant Nuclei for Mutation Testing
Viray H, Coulter M, Li K, Lane K, Madan A, Mitchell K, Schalper K, Hoyt C, Rimm DL. Automated Objective Determination of Percentage of Malignant Nuclei for Mutation Testing. Applied Immunohistochemistry & Molecular Morphology 2014, 22: 363-371. PMID: 24162261, PMCID: PMC3999345, DOI: 10.1097/pai.0b013e318299a1f6.Peer-Reviewed Original ResearchConceptsCriterion standardMalignant cellsMalignant nucleiCompanion diagnostic testsTumor cell percentageMutation testingEosin-stained tissuesCell percentageInForm softwareHistologic specimensTumor tissueColon adenocarcinomaTumor cellsDiagnostic testsPotential future toolDNA mutation testingTissue sectionsContinuous variablesFurther validationPathologist estimationAnalytic sensitivityVariant resultsDNA mutationsBenign nucleiTissueMacrophage expression of tartrate-resistant acid phosphatase as a prognostic indicator in colon cancer
How J, Brown JR, Saylor S, Rimm DL. Macrophage expression of tartrate-resistant acid phosphatase as a prognostic indicator in colon cancer. Histochemistry And Cell Biology 2014, 142: 195-204. PMID: 24429833, PMCID: PMC4101067, DOI: 10.1007/s00418-014-1181-6.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAdenocarcinomaAdultAgedAged, 80 and overAntigens, CDAntigens, Differentiation, MyelomonocyticBiomarkers, TumorColonic NeoplasmsFemaleHumansIsoenzymesMacrophagesMaleMiddle AgedReceptors, Cell SurfaceTartrate-Resistant Acid PhosphataseTissue Array AnalysisTreatment OutcomeYoung AdultConceptsColorectal cancer patientsMacrophage expressionResistant acid phosphataseColon cancerCancer patientsTRAP expressionYale-New Haven HospitalDisease-specific deathPan-macrophage markerRisk reductionPrognostic indicatorCancer survivalColorectal adenocarcinomaM2 markersImproved outcomesTissue microarrayImmunohistochemical analysisSecond cohortSurvival analysisPatientsPotential biomarkersQuantitative immunofluorescenceCancerAcid phosphataseOld cases
2013
A Prospective, Multi-Institutional Diagnostic Trial to Determine Pathologist Accuracy in Estimation of Percentage of Malignant Cells
Viray H, Li K, Long TA, Vasalos P, Bridge JA, Jennings LJ, Halling KC, Hameed M, Rimm DL. A Prospective, Multi-Institutional Diagnostic Trial to Determine Pathologist Accuracy in Estimation of Percentage of Malignant Cells. Archives Of Pathology & Laboratory Medicine 2013, 137: 1545-9. PMID: 24168492, DOI: 10.5858/arpa.2012-0561-cp.Peer-Reviewed Original ResearchConceptsFalse-negative test resultsMalignant cellsMulti-institutional studyColon tissue specimensCriterion standardPatient careTissue specimensTumor tissueDiagnostic trialPathologists' accuracyGenetic alterationsNuclear countsPathologist estimationEstimation of percentageVisual estimationCurrent studyCellsTesting failures
2007
Definition of a direct extracellular interaction between Met and E‐cadherin
Reshetnikova G, Troyanovsky S, Rimm DL. Definition of a direct extracellular interaction between Met and E‐cadherin. Cell Biology International 2007, 31: 366-373. PMID: 17336101, DOI: 10.1016/j.cellbi.2007.01.022.Peer-Reviewed Original ResearchConceptsBT-549 cellsE-cadherinCadherin-dependent cell-cell contactsHT-29 cellsE-cadherin interactsHepatocyte growth factorCell-cell adhesionCell-cell contactCross-linking studiesDirect extracellular interactionTyrosine kinase receptor expressionExtracellular interactionsMolecular mechanismsExtracellular domainIntracellular compartmentsPhysical interactionCellular presentationFirst evidenceGrowth factorCellsBT-549HT-29ExpressionReceptor expressionMetS
2006
Reciprocal regulation of RhoA and RhoC characterizes the EMT and identifies RhoC as a prognostic marker of colon carcinoma
Bellovin DI, Simpson KJ, Danilov T, Maynard E, Rimm DL, Oettgen P, Mercurio AM. Reciprocal regulation of RhoA and RhoC characterizes the EMT and identifies RhoC as a prognostic marker of colon carcinoma. Oncogene 2006, 25: 6959-6967. PMID: 16715134, DOI: 10.1038/sj.onc.1209682.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorCadherinsCell Line, TumorColonic NeoplasmsEnzyme ActivationEpithelial CellsHumansImmunohistochemistryImmunoprecipitationNeoplasm InvasivenessPrognosisPromoter Regions, GeneticProto-Oncogene Protein c-ets-1Reverse Transcriptase Polymerase Chain ReactionRho GTP-Binding ProteinsRhoA GTP-Binding ProteinRhoC GTP-Binding ProteinRNA, Small InterferingTranscription, GeneticConceptsColon carcinomaRhoC expressionPrognostic markerRhoC protein expressionE-cadherinET-1 binding sitesClinical outcomesPoor outcomeColon cancer cellsColorectal tumorsET-1Colon cancerUse of shRNAMesenchymal transitionExpression correlatesCarcinomaAberrant expressionHigh expressionProtein expressionCancer cellsMesenchymal characteristicsEMTSubsequent activationReciprocal regulationCell migration
2005
Detection of malignancy in cytology specimens using spectral–spatial analysis
Angeletti C, Harvey NR, Khomitch V, Fischer AH, Levenson RM, Rimm DL. Detection of malignancy in cytology specimens using spectral–spatial analysis. Laboratory Investigation 2005, 85: 1555-1564. PMID: 16200074, DOI: 10.1038/labinvest.3700357.Peer-Reviewed Original ResearchAltered Localization of p120 Catenin During Epithelial to Mesenchymal Transition of Colon Carcinoma Is Prognostic for Aggressive Disease
Bellovin DI, Bates RC, Muzikansky A, Rimm DL, Mercurio AM. Altered Localization of p120 Catenin During Epithelial to Mesenchymal Transition of Colon Carcinoma Is Prognostic for Aggressive Disease. Cancer Research 2005, 65: 10938-10945. PMID: 16322241, DOI: 10.1158/0008-5472.can-05-1947.Peer-Reviewed Original ResearchConceptsSurvival timeMesenchymal transitionLymph node metastasisColorectal cancer progressionPoor patient outcomesE-cadherinLate-stage tumorsPatient survival timePost-EMT cellsP120ctn expressionAltered localizationLymph nodesNode metastasisAggressive diseaseTumor stagePrimary tumorTumor necrosisColorectal carcinomaPatient outcomesColon carcinoma cellsE-cadherin lossCytoplasmic stainingColon carcinomaCancer progressionCarcinoma cells
1998
A Mutation in α-Catenin Disrupts Adhesion in Clone A Cells Without Perturbing its Actin and β-Catenin Binding Activity
Roe S, Koslov E, Rimm D. A Mutation in α-Catenin Disrupts Adhesion in Clone A Cells Without Perturbing its Actin and β-Catenin Binding Activity. Cell Communication & Adhesion 1998, 5: 283-296. PMID: 9762469, DOI: 10.3109/15419069809040298.Peer-Reviewed Original ResearchMeSH KeywordsActinsAlpha CateninBeta CateninCadherinsCell AdhesionCloning, MolecularColonic NeoplasmsCytoskeletal ProteinsCytoskeletonDesmoplakinsExonsGamma CateninHeLa CellsHumansIntercellular JunctionsMutationOctoxynolPrecipitin TestsProtein BindingRecombinant Fusion ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSequence Analysis, DNASolubilityTrans-ActivatorsTransfectionTumor Cells, CulturedConceptsN-terminusE-cadherin-catenin complexBundles F-actinCo-sedimentation assaysCell-cell adhesionFull-length proteinClone A cellsCo-precipitation experimentsInternal deletion mutationsWhole cell lysatesAdhesive complexesMutant proteinsA mutantsMutant bindsHuman colon carcinoma cell lineColon carcinoma cell lineMutant formsLength proteinWild typeCytoplasmic connectionsF-actinAdhesive phenotypeDeletion mutationsCell lysatesCarcinoma cell lines
1995
Autoantibodies specific for villin found in patients with colon cancer and other colitides
Rimm D, Holland T, Morrow J, Anderson J. Autoantibodies specific for villin found in patients with colon cancer and other colitides. Digestive Diseases And Sciences 1995, 40: 389-395. PMID: 7851204, DOI: 10.1007/bf02065426.Peer-Reviewed Original ResearchConceptsColon cancerActive autoimmune responseSpecific gastrointestinal diseasesLevels of autoantibodiesColon cancer patients´ seraCancer patient seraIntestinal epithelial cellsCryptic antigensAutoimmune responseGastrointestinal pathologyColonic diseaseGastrointestinal diseasesPatient seraNormal controlsPathological significanceWestern blotDisease statesEpithelial cellsNoninvasive approachUnique noninvasive approachAntibodiesAutoantibodiesBrush borderPatientsCancer