1990
Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. II. Assembly properties of tails with NH2- and COOH-terminal deletions.
Sinard JH, Rimm DL, Pollard TD. Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. II. Assembly properties of tails with NH2- and COOH-terminal deletions. Journal Of Cell Biology 1990, 111: 2417-2426. PMID: 2177477, PMCID: PMC2116375, DOI: 10.1083/jcb.111.6.2417.Peer-Reviewed Original ResearchMeSH KeywordsAcanthamoebaAnimalsBase SequenceBinding SitesChromatographyChromatography, DEAE-CelluloseChromatography, GelChromosome DeletionCloning, MolecularDurapatiteElectrophoresis, Polyacrylamide GelEscherichia coliHydroxyapatitesKineticsMacromolecular SubstancesMagnesiumMicroscopy, ElectronMolecular Sequence DataMolecular WeightMyosinsPotassium ChlorideRecombinant Fusion ProteinsScattering, RadiationConceptsFusion proteinMyosin IIMyosin-II tailAntiparallel tetramersAmino acidsAmino acid residuesNative myosin IIRecombinant fusion proteinSequence altersAcid residuesTail sequencesNH2-terminalNonhelical domainAcanthamoeba myosin IIFunctional regionsProteinParacrystal formationAntiparallel dimerAssembly propertiesDimerization mechanismResiduesTerminal deletionDeletionAssemblyTight packing
1989
Structure-Function Studies of the Actin Filament System of Acanthamoeba
Pollard T, Magnus K, Doberstein S, Goldschmidt-Clermont P, Kaiser D, Machesky L, Maciver S, Rimm D, Wachsstock D. Structure-Function Studies of the Actin Filament System of Acanthamoeba. Springer Series In Biophysics 1989, 3: 271-279. DOI: 10.1007/978-3-642-73925-5_50.Peer-Reviewed Original ResearchRegulatory proteinsActin polymerizationEssential protein componentsActin filament systemStructure-function studiesMajor functional classesMechanism of actionActin systemAccessory proteinsProtein componentsPrimary structureFilament systemCellular levelProteinCytoplasmic matrixOverall mechanismCellsMechanismFunctional classAssemblyAcanthamoeba