Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies
Bradshaw EM, Kent SC, Tripuraneni V, Orban T, Ploegh HL, Hafler DA, Love JC. Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies. Clinical Immunology 2008, 129: 10-18. PMID: 18675591, PMCID: PMC2577144, DOI: 10.1016/j.clim.2008.06.009.Peer-Reviewed Original ResearchConceptsHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsType 1 diabetic subjectsAntigen-reactive antibodiesBlood mononuclear cellsAntigen-specific antibodiesAnti-insulin antibodiesCell surface determinantsDiabetic subjectsIL-6Mononuclear cellsPositive titersIgG isotypeB cellsHuman lymphocytesSurface determinantsCytokinesAntibodiesClinical samplesHematopoietic cellsRare populationCellsMarkersHuman cellsLymphocytes