2019
Filamin A mediates isotropic distribution of applied force across the actin network
Kumar A, Shutova MS, Tanaka K, Iwamoto DV, Calderwood DA, Svitkina TM, Schwartz MA. Filamin A mediates isotropic distribution of applied force across the actin network. Journal Of Cell Biology 2019, 218: 2481-2491. PMID: 31315944, PMCID: PMC6683746, DOI: 10.1083/jcb.201901086.Peer-Reviewed Original ResearchConceptsTalin tension sensorStress fibersActin networkFilamin ACortical actin networkCortical actin filamentsIntegrin-mediated adhesionActin cytoskeletonFocal adhesionsCortical actinFLNA knockdownActin filamentsTalinKnockdownCell sensingDirection of stretchTension sensorPhysiology of muscleUniaxial stretchForce transmissionCytoskeletonStrainsStretchAdhesionReexpression
2012
Macrophage Mesenchymal Migration Requires Podosome Stabilization by Filamin A*
Guiet R, Vérollet C, Lamsoul I, Cougoule C, Poincloux R, Labrousse A, Calderwood DA, Glogauer M, Lutz PG, Maridonneau-Parini I. Macrophage Mesenchymal Migration Requires Podosome Stabilization by Filamin A*. Journal Of Biological Chemistry 2012, 287: 13051-13062. PMID: 22334688, PMCID: PMC3339984, DOI: 10.1074/jbc.m111.307124.Peer-Reviewed Original ResearchConceptsFilamin AMesenchymal migrationEmbryonic developmentPodosome rosette formationCell migrationMesenchymal migration modeCertain cell typesPodosome stabilityScaffold proteinActin polymerizationAmoeboid migrationNull mutationPodosomesActin filamentsMigratory cellsAmoeboid modeCell typesOrgan defectsMigration modesNew functionsThree-dimensional environmentMutationsProteaseStrong consequencesFLNA mutationsFilamin A controls matrix metalloproteinase activity and regulates cell invasion in human fibrosarcoma cells
Baldassarre M, Razinia Z, Brahme NN, Buccione R, Calderwood DA. Filamin A controls matrix metalloproteinase activity and regulates cell invasion in human fibrosarcoma cells. Journal Of Cell Science 2012, 125: 3858-3869. PMID: 22595522, PMCID: PMC3462082, DOI: 10.1242/jcs.104018.Peer-Reviewed Original ResearchMeSH KeywordsActinsCell AdhesionCell Line, TumorCell MovementContractile ProteinsEnzyme ActivationExtracellular MatrixFibrosarcomaFilaminsGene Knockdown TechniquesHumansIntegrinsMatrix Metalloproteinase 14Matrix Metalloproteinase 2Microfilament ProteinsNeoplasm InvasivenessPhenotypeProtein Structure, TertiaryConceptsFilamin AActin cytoskeletonCell invasionActin-binding domainCell surface adhesion proteinsControls cell motilityActin-binding proteinsIntegrin adhesion receptorsRandom cell migrationAbility of cellsArray of intracellularBreast cancer lossSurface adhesion proteinsHuman fibrosarcoma cellsExtracellular matrix degradationMatrix metalloproteinase activityFilamin expressionKnockdown cellsAdhesion proteinsCell motilityMetalloproteinase activityActin filamentsAdhesion receptorsFilaminECM remodeling
2009
Filamin A–β1 Integrin Complex Tunes Epithelial Cell Response to Matrix Tension
Gehler S, Baldassarre M, Lad Y, Leight JL, Wozniak MA, Riching KM, Eliceiri KW, Weaver VM, Calderwood DA, Keely PJ. Filamin A–β1 Integrin Complex Tunes Epithelial Cell Response to Matrix Tension. Molecular Biology Of The Cell 2009, 20: 3224-3238. PMID: 19458194, PMCID: PMC2710838, DOI: 10.1091/mbc.e08-12-1186.Peer-Reviewed Original ResearchConceptsFilamin AExtracellular matrixProtein filamin AHigh-density gelsMatrix tensionCollagen gelsMechanosensitive complexBreast epithelial cellsCellular contractilityMatrix stiffnessMorphogenesisEpithelial cell responsesCell typesDuctal morphogenesisEpithelial cellsCellsCollagen matrixGel contractionActinCollagen remodelingIntegrinsCell responsesCollagen fibrilsRemodelingGelThe Role of FilGAP-Filamin A Interactions in Mechanoprotection
Shifrin Y, Arora PD, Ohta Y, Calderwood DA, McCulloch CA. The Role of FilGAP-Filamin A Interactions in Mechanoprotection. Molecular Biology Of The Cell 2009, 20: 1269-1279. PMID: 19144823, PMCID: PMC2649276, DOI: 10.1091/mbc.e08-08-0872.Peer-Reviewed Original Research
2008
ASB2 targets filamins A and B to proteasomal degradation
Heuzé ML, Lamsoul I, Baldassarre M, Lad Y, Lévêque S, Razinia Z, Moog-Lutz C, Calderwood DA, Lutz PG. ASB2 targets filamins A and B to proteasomal degradation. Blood 2008, 112: 5130-5140. PMID: 18799729, PMCID: PMC2597609, DOI: 10.1182/blood-2007-12-128744.Peer-Reviewed Original ResearchConceptsAnkyrin repeat-containing proteinFilamin AE3 ubiquitin ligase complexActin-binding protein filamin AFilamin degradationRepeat-containing proteinUbiquitin ligase complexSeries of proliferationHematopoietic cell differentiationProtein filamin AAcid-induced differentiationSuppressor of cytokineLigase complexSpecificity subunitLeukemia cellsHematopoietic differentiationHematopoietic progenitor cellsProteasomal degradationMolecular basisAcute promyelocytic leukemia cellsSpecific proteinsCell spreadingPromyelocytic leukemia cellsArrest of differentiationCell differentiation
2006
The Molecular Basis of Filamin Binding to Integrins and Competition with Talin
Kiema T, Lad Y, Jiang P, Oxley CL, Baldassarre M, Wegener KL, Campbell ID, Ylänne J, Calderwood DA. The Molecular Basis of Filamin Binding to Integrins and Competition with Talin. Molecular Cell 2006, 21: 337-347. PMID: 16455489, DOI: 10.1016/j.molcel.2006.01.011.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesCalpainContractile ProteinsCrystallography, X-RayFilaminsIntegrin beta ChainsMiceMicrofilament ProteinsModels, MolecularMolecular Sequence DataNIH 3T3 CellsNuclear Magnetic Resonance, BiomolecularProtein BindingProtein ConformationProtein Structure, TertiaryRecombinant Fusion ProteinsReproducibility of ResultsSequence Homology, Amino AcidTalinConceptsAdhesion receptorsTalin-dependent integrin activationActin-crosslinking proteinsIntegrin adhesion receptorsHigh-resolution structuresFilamin bindingExtended beta strandActin cytoskeletonIntegrin tailsMultiple transmembraneMolecular basisStrands CBeta strandsDomain interactionsBiochemical signalsIntegrin functionIntegrin activationFilamin ATalinCell membraneTail formsCytoskeletonProteinBinding sitesFilamin