2019
Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior
Blanco-Centurion C, Luo S, Spergel DJ, Vidal-Ortiz A, Oprisan S, Van den Pol AN, Liu M, Shiromani PJ. Dynamic Network Activation of Hypothalamic MCH Neurons in REM Sleep and Exploratory Behavior. Journal Of Neuroscience 2019, 39: 4986-4998. PMID: 31036764, PMCID: PMC6670248, DOI: 10.1523/jneurosci.0305-19.2019.Peer-Reviewed Original ResearchConceptsMelanin-concentrating hormoneRapid eye movement (REM) sleepMCH neuronsEye movement sleepREM sleepHypothalamic neuronsMovement sleepHypothalamic melanin-concentrating hormone (MCH) neuronsMelanin-concentrating hormone (MCH) neuronsNeuropeptide melanin-concentrating hormoneHypothalamic MCH neuronsIndividual hypothalamic neuronsMost brain neuronsFunctional activity mapsExploratory behaviorNon-REM sleepCalcium indicator GCaMP6Single neuron activityHormone neuronsNetwork activationBrain neuronsElectrophysiology studySame neuronsQuiet wakingIndicator GCaMP6s
2012
Effects of Leptin and Melanocortin Signaling Interactions on Pubertal Development and Reproduction
Israel DD, Sheffer-Babila S, de Luca C, Jo YH, Liu SM, Xia Q, Spergel DJ, Dun SL, Dun NJ, Chua SC. Effects of Leptin and Melanocortin Signaling Interactions on Pubertal Development and Reproduction. Endocrinology 2012, 153: 2408-2419. PMID: 22408174, PMCID: PMC3381095, DOI: 10.1210/en.2011-1822.Peer-Reviewed Original ResearchConceptsGnRH neuron activityNeuron activityGnRH neuronsFemale db/db miceDb/db miceMelanocortin 4 receptor activationNeuropeptides neuropeptide YAgouti-related peptideAction potential firingEffects of leptinC-fos expressionMelanocortin-4 receptorInitiation of pubertyHypothalamic hypogonadismDb miceNeuropeptide YMelanocortin agonistsLeptin receptorReceptor activationPubertal developmentΑ-MSHPubertal onsetMelanocortinLeptinDownstream mediator
2005
Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons.
Shimshek DR, Bus T, Grinevich V, Single FN, Mack V, Sprengel R, Spergel DJ, Seeburg PH. Impaired reproductive behavior by lack of GluR-B containing AMPA receptors but not of NMDA receptors in hypothalamic and septal neurons. Endocrinology 2005, 20: 219-31. PMID: 16099814, DOI: 10.1210/me.2005-0262.Peer-Reviewed Original ResearchConceptsAMPA receptorsNMDA receptorsGnRH secretionMale miceN-methyl-D-aspartate receptorsLimbic system neuronsSerum testosterone levelsIsoxazolepropionic acid (AMPA) receptorsIonotropic glutamate receptorsNormal litter sizeMale sexual behaviorGnRH neuronsSeptal neuronsSystem neuronsOvarian morphologyGlutamate receptorsSeptal areaTestosterone levelsControl littermatesMale littermatesPup retrievalMajor subtypesTestis weightMaternal aggressionAcid receptors
2003
A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice
Mitchell V, Loyens A, Spergel DJ, Flactif M, Poulain P, Tramu G, Beauvillain JC. A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice. Neuroendocrinology 2003, 77: 198-207. PMID: 12673053, DOI: 10.1159/000069511.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArcuate Nucleus of HypothalamusBrain MappingDopamineEstrogen Receptor alphaFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsHypothalamusLuminescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, ConfocalNerve NetNeural PathwaysNeuronsReceptors, EstrogenTyrosine 3-MonooxygenaseConceptsGnRH-green fluorescent protein transgenic miceTH-ir neuronsTIDA neuronsArcuate nucleusGnRH neuronsDopaminergic neuronsFemale miceTransgenic miceTyrosine hydroxylase-immunoreactive neuronsTH monoclonal antibodyTuberoinfundibular dopaminergic neuronsHydroxylase-immunoreactive neuronsRole of estradiolAdult female miceDouble immunohistochemical stainingEstrogen receptor αGonadotropin-releasing hormone pathwayERalpha activationGnRH fibersImmunoreactive neuronsImmunohistochemical stainingBrain sectionsConfocal microscopic studiesReceptor αEstrus stage
1999
GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice
Spergel D, Krüth U, Hanley D, Sprengel R, Seeburg P. GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice. Journal Of Neuroscience 1999, 19: 2037-2050. PMID: 10066257, PMCID: PMC6782541, DOI: 10.1523/jneurosci.19-06-02037.1999.Peer-Reviewed Original ResearchConceptsGnRH neuronsPreoptic areaNucleated patchesWhole-cell recordingsApplication of GABANMDA receptor channelsGanglion terminaleHormone neuronsOrganum vasculosumSeptal nucleusDiagonal bandLamina terminalisNeuronal somataAxon terminalsOlfactory bulbMedian eminenceTransgenic miceFiring patternsNeuronsNeuronal dendritesReceptor channelsGABAGreen fluorescent proteinL-glutamateAMPA
1995
Autocrine actions of endothelin-1 as a growth factor in human ovarian carcinoma cells.
Bagnato A, Tecce R, Moretti C, Di Castro V, Spergel D, Catt K. Autocrine actions of endothelin-1 as a growth factor in human ovarian carcinoma cells. Clinical Cancer Research 1995, 1: 1059-66. PMID: 9816080.Peer-Reviewed Original ResearchConceptsOvarian carcinoma cellsHuman ovarian carcinoma cellsET-1ETA receptorsEndothelin-1Carcinoma cellsTumor cellsCell linesGrowth factorCertain ovarian tumorsET-1 participatesReceptor-mediated actionsOVCA 433 cellsOvarian tumor cell linesCalcium signalingDose-dependent increaseRadioligand binding studiesOvarian tumor cellsAutocrine growth factorOvarian cancer cellsSites/cellBasal growth rateAuthentic ET-1BQ-123ET receptors
1993
Optical Measurements of Micro-Vascular Oxygen Pressure and Intracellular pH in the Cat Carotid Body: Testing Hypotheses of Oxygen Chemoreception
Rumsey WL, Iturriaga R, Spergel D, Lahiri S, Wilson DF. Optical Measurements of Micro-Vascular Oxygen Pressure and Intracellular pH in the Cat Carotid Body: Testing Hypotheses of Oxygen Chemoreception. Advances In Experimental Medicine And Biology 1993, 337: 197-203. PMID: 8109402, DOI: 10.1007/978-1-4615-2966-8_28.Chapters
1992
Intracellular pH and oxygen chemoreception in the cat carotid body in vitro
Iturriaga R, Rumsey WL, Lahiri S, Spergel D, Wilson DF. Intracellular pH and oxygen chemoreception in the cat carotid body in vitro. Journal Of Applied Physiology 1992, 72: 2259-2266. PMID: 1629081, DOI: 10.1152/jappl.1992.72.6.2259.Peer-Reviewed Original ResearchOptical Measurements of Oxygen and Electrical Measurements of Oxygen Chemoreception in the Cat Carotid Body
Rumsey W, Lahiri S, Iturriaga R, Mokashi A, Spergel D, Wilson D. Optical Measurements of Oxygen and Electrical Measurements of Oxygen Chemoreception in the Cat Carotid Body. Advances In Experimental Medicine And Biology 1992, 317: 387-395. PMID: 1288149, DOI: 10.1007/978-1-4615-3428-0_43.Chapters
1991
Optical measurements of the dependence of chemoreception on oxygen pressure in the cat carotid body
Rumsey WL, Iturriaga R, Spergel D, Lahiri S, Wilson DF. Optical measurements of the dependence of chemoreception on oxygen pressure in the cat carotid body. American Journal Of Physiology 1991, 261: c614-c622. PMID: 1928325, DOI: 10.1152/ajpcell.1991.261.4.c614.Peer-Reviewed Original ResearchConceptsCarotid bodyCat carotid bodyChemosensory dischargeCarotid arteryCarotid sinus nerve dischargeSinus nerve dischargeCommon carotid arteryTorr/sMicrovascular PO2Nerve dischargeOxygen-dependent quenchingNeural dischargePerfusate flowTyrode's solutionIntravascular PO2Oxygen metabolismOxygen chemoreceptionPerfusateNeural activityArteryExchange vesselsPO2 valuesPO2Brief interruptionIn vitro perfused-superfused cat carotid body for physiological and pharmacological studies
Iturriaga R, Rumsey WL, Mokashi A, Spergel D, Wilson DF, Lahiri S. In vitro perfused-superfused cat carotid body for physiological and pharmacological studies. Journal Of Applied Physiology 1991, 70: 1393-1400. PMID: 1903380, DOI: 10.1152/jappl.1991.70.3.1393.Peer-Reviewed Original ResearchConceptsChemosensory dischargeCarotid bodyPentobarbital sodium-anesthetized catsWhole carotid sinus nerveCarotid sinus nerveInjections of nicotineCarotid body preparationCat carotid bodyRestoration of flowDose-dependent mannerSinus nerveNormoxic perfusionPerfusate PO2Pharmacological stimuliPerfusate flowInitial responsivenessTyrode's solutionPharmacological studiesCarotid bifurcationPerfusionCO2 stimulusBasal activityChemosensory responsesNicotineBody preparation