2009
Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse
Ward DR, Dear FM, Ward IA, Anderson SI, Spergel DJ, Smith PA, Ebling FJ. Innervation of Gonadotropin-Releasing Hormone Neurons by Peptidergic Neurons Conveying Circadian or Energy Balance Information in the Mouse. PLOS ONE 2009, 4: e5322. PMID: 19390688, PMCID: PMC2669176, DOI: 10.1371/journal.pone.0005322.Peer-Reviewed Original ResearchConceptsGnRH neuronsVasoactive intestinal peptidePeptidergic neuronsGonadotropin-Releasing Hormone NeuronsGnRH secretory systemVIP-IR fibersGonadotropin-releasing hormoneHypothalamic peptide systemsHormone neuronsBasal forebrainIntestinal peptideHypothalamic neuropeptidesPeptidergic fibersReproductive axisTransgenic miceModulatory inputsNeuronsCircadian timing informationPeptidergic signalsMetabolic statusMiceCircadian informationInnervationSignificant proportionPeptide system
2007
Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty
Spergel DJ. Calcium and Small-Conductance Calcium-Activated Potassium Channels in Gonadotropin-Releasing Hormone Neurons before, during, and after Puberty. Endocrinology 2007, 148: 2383-2390. PMID: 17289846, PMCID: PMC3315592, DOI: 10.1210/en.2006-1693.Peer-Reviewed Original ResearchConceptsAdult GnRH neuronsSK channel expressionGnRH neuronsGnRH secretionSK currentPubertal increaseGnRH-green fluorescent protein transgenic miceChannel expressionSmall-conductance calcium-activated potassium channelsGonadotropin-releasing hormone (GnRH) neuronsSK channel blocker apaminCalcium-activated potassium channelsCytoplasmic freeChannel blocker apaminExcitatory synaptic inputsWhole-cell techniqueSK channel subtypesHormone neuronsBlocker apaminKisspeptin stimulationChannel blockersSK channelsBrain slicesChannel subtypesSynaptic inputs
2004
Neurotrophic effects of BDNF on embryonic gonadotropin‐releasing hormone (GnRH) neurons
Cronin AS, Horan TL, Spergel DJ, Brooks AN, Hastings MH, Ebling FJ. Neurotrophic effects of BDNF on embryonic gonadotropin‐releasing hormone (GnRH) neurons. European Journal Of Neuroscience 2004, 20: 338-344. PMID: 15233743, DOI: 10.1111/j.1460-9568.2004.03490.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainBrain-Derived Neurotrophic FactorCell CountCells, CulturedCyclic AMP Response Element-Binding ProteinDose-Response Relationship, DrugDrug InteractionsEmbryo, MammalianGonadotropin-Releasing HormoneGreen Fluorescent ProteinsImmunoenzyme TechniquesImmunohistochemistryLuminescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMitogen-Activated Protein KinasesNeuritesNeuronsOligodeoxyribonucleotides, AntisensePotassium ChlorideReceptor, trkBConceptsBrain-derived neurotrophic factorGnRH cellsNeurotrophic factorMedian eminenceGonadotropin-releasing hormone (GnRH) neuronsNeurite outgrowthGnRH secretory systemGnRH-immunoreactive cellsGonadotropin-releasing hormoneDose-dependent increaseResponse element-binding proteinPhosphorylation of ERKHormone neuronsBDNF treatmentGnRH neuronsNeurotrophic effectsPreoptic areaNeurons migrateReproductive axisElement-binding proteinOlfactory placodeOlfactory tissuePrimary culturesERK pathwayGnRH
2003
A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice
Mitchell V, Loyens A, Spergel DJ, Flactif M, Poulain P, Tramu G, Beauvillain JC. A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice. Neuroendocrinology 2003, 77: 198-207. PMID: 12673053, DOI: 10.1159/000069511.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArcuate Nucleus of HypothalamusBrain MappingDopamineEstrogen Receptor alphaFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsHypothalamusLuminescent ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, ConfocalNerve NetNeural PathwaysNeuronsReceptors, EstrogenTyrosine 3-MonooxygenaseConceptsGnRH-green fluorescent protein transgenic miceTH-ir neuronsTIDA neuronsArcuate nucleusGnRH neuronsDopaminergic neuronsFemale miceTransgenic miceTyrosine hydroxylase-immunoreactive neuronsTH monoclonal antibodyTuberoinfundibular dopaminergic neuronsHydroxylase-immunoreactive neuronsRole of estradiolAdult female miceDouble immunohistochemical stainingEstrogen receptor αGonadotropin-releasing hormone pathwayERalpha activationGnRH fibersImmunoreactive neuronsImmunohistochemical stainingBrain sectionsConfocal microscopic studiesReceptor αEstrus stage
1999
GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice
Spergel D, Krüth U, Hanley D, Sprengel R, Seeburg P. GABA- and Glutamate-Activated Channels in Green Fluorescent Protein-Tagged Gonadotropin-Releasing Hormone Neurons in Transgenic Mice. Journal Of Neuroscience 1999, 19: 2037-2050. PMID: 10066257, PMCID: PMC6782541, DOI: 10.1523/jneurosci.19-06-02037.1999.Peer-Reviewed Original ResearchConceptsGnRH neuronsPreoptic areaNucleated patchesWhole-cell recordingsApplication of GABANMDA receptor channelsGanglion terminaleHormone neuronsOrganum vasculosumSeptal nucleusDiagonal bandLamina terminalisNeuronal somataAxon terminalsOlfactory bulbMedian eminenceTransgenic miceFiring patternsNeuronsNeuronal dendritesReceptor channelsGABAGreen fluorescent proteinL-glutamateAMPA