1998
Role of Glutamine 17 of the Bovine Papillomavirus E5 Protein in Platelet-Derived Growth Factor β Receptor Activation and Cell Transformation
Klein O, Polack G, Surti T, Kegler-Ebo D, Smith S, DiMaio D. Role of Glutamine 17 of the Bovine Papillomavirus E5 Protein in Platelet-Derived Growth Factor β Receptor Activation and Cell Transformation. Journal Of Virology 1998, 72: 8921-8932. PMID: 9765437, PMCID: PMC110309, DOI: 10.1128/jvi.72.11.8921-8932.1998.Peer-Reviewed Original ResearchConceptsBovine papillomavirus E5 proteinPDGF beta receptorE5 proteinTransform cellsCellular platelet-derived growth factor (PDGF) beta receptorAmino acidsBa/F3 hematopoietic cellsPosition 17Cell transformationPlatelet-derived growth factor beta receptorHomodimeric transmembrane proteinReceptor tyrosine phosphorylationGrowth factor beta receptorReceptor tyrosine kinasesPDGF receptor tyrosine kinaseReceptor activationPossible amino acidsBeta receptorsStable complexesComplex formationMutant proteinsTransmembrane domainTransmembrane proteinGrowth factor-beta (TGF-beta) receptor activationTyrosine phosphorylation
1996
Activation of the endogenous p53 growth inhibitory pathway in HeLa cervical carcinoma cells by expression of the bovine papillomavirus E2 gene.
Hwang E, Naeger L, DiMaio D. Activation of the endogenous p53 growth inhibitory pathway in HeLa cervical carcinoma cells by expression of the bovine papillomavirus E2 gene. Oncogene 1996, 12: 795-803. PMID: 8632901.Peer-Reviewed Original ResearchMeSH KeywordsBovine papillomavirus 1CDC2-CDC28 KinasesCell DivisionCyclin-Dependent Kinase 2Cyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent KinasesCyclinsDNA ReplicationDNA-Binding ProteinsEnzyme InhibitorsFemaleGene Expression Regulation, NeoplasticGene Expression Regulation, ViralGenes, ViralHeLa CellsHumansModels, BiologicalPhosphorylationProtein Serine-Threonine KinasesTumor Suppressor Protein p53Uterine Cervical NeoplasmsViral ProteinsConceptsTumor suppressor proteinGrowth inhibitory pathwaySuppressor proteinHeLa cellsP21/waf1Kinase activityE2 geneBPV E2 proteinP53 tumor suppressor proteinCdk2/cyclin E kinase activityCyclin-dependent kinase inhibitorGrowth regulatory pathwaysHeLa cervical carcinoma cellsP53-responsive genesCell cycle regulatory proteinsCDK kinase activityCyclin E kinase activityCycle regulatory proteinsDependent kinase inhibitorG1 cell cycle regulatory proteinsB-MybTranscription factorsRegulatory proteinsRegulatory pathwaysP105Rb
1993
Inhibition of cervical carcinoma cell line proliferation by the introduction of a bovine papillomavirus regulatory gene
Hwang E, Riese D, Settleman J, Nilson L, Honig J, Flynn S, DiMaio D. Inhibition of cervical carcinoma cell line proliferation by the introduction of a bovine papillomavirus regulatory gene. Journal Of Virology 1993, 67: 3720-3729. PMID: 8389903, PMCID: PMC237735, DOI: 10.1128/jvi.67.7.3720-3729.1993.Peer-Reviewed Original ResearchConceptsBPV E2 proteinGene expressionCervical carcinoma cell linesRegulatory genesHPV gene expressionCarcinoma cell linesCell linesE2 proteinHeLa cellsConsequence of abrogationP53 tumor suppressor proteinHPV18 E6Tumor suppressor proteinE2 geneDestabilization of p53HT-3 cellsGrowth inhibitionBPV E2Simian virus 40Suppressor proteinEpithelial cell lineHuman papillomavirus E6Cell cycleGenesS phase
1992
The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits
Brandsma J, Yang Z, DiMaio D, Barthold S, Johnson E, Xiao W. The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits. Journal Of Virology 1992, 66: 6204-6207. PMID: 1326666, PMCID: PMC283673, DOI: 10.1128/jvi.66.10.6204-6207.1992.Peer-Reviewed Original ResearchConceptsE5 ORFCRPV DNACRPV genomeCottontail rabbit papillomavirusE5 mutantsFrameshift mutantsBiological functionsORFRabbit papillomavirusMutantsMutant DNATransition mutationsPapilloma formationDNA preparationsDNABiochemical analysisGenomeE5 regionDomestic rabbitsFrame mutationsMutationsCodonGenesInduction of papillomasFrameshift
1991
Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16
Leptak C, Cajal S, Kulke R, Horwitz B, Riese D, Dotto G, DiMaio D. Tumorigenic transformation of murine keratinocytes by the E5 genes of bovine papillomavirus type 1 and human papillomavirus type 16. Journal Of Virology 1991, 65: 7078-7083. PMID: 1658398, PMCID: PMC250837, DOI: 10.1128/jvi.65.12.7078-7083.1991.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1E5 genePapillomavirus type 1Human Papillomavirus Type 16 E5 GeneTumorigenic transformationHost epithelial cellsExpression vectorRecombinant virusesRetroviral expression vectorMurine keratinocytesBiological propertiesTumorigenic cellsMurine epidermal keratinocytesGenesMurine fibroblastsFrameshift mutationCultured linesHuman papillomavirus type 16Cell linesEpithelial cellsPapillomavirus type 16Epidermal keratinocytesRetrovirusesCellsKeratinocytesBiological properties of the deer papillomavirus E5 gene in mouse C127 cells: growth transformation, induction of DNA synthesis, and activation of the platelet-derived growth factor receptor
Kulke R, DiMaio D. Biological properties of the deer papillomavirus E5 gene in mouse C127 cells: growth transformation, induction of DNA synthesis, and activation of the platelet-derived growth factor receptor. Journal Of Virology 1991, 65: 4943-4949. PMID: 1651413, PMCID: PMC248956, DOI: 10.1128/jvi.65.9.4943-4949.1991.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBlotting, NorthernCell Transformation, ViralCloning, MolecularDeerDNAGene ExpressionGenes, ViralIn Vitro TechniquesMiceMolecular Sequence DataOncogene Proteins, ViralPapillomaviridaePlatelet-Derived Growth FactorReceptors, Cell SurfaceReceptors, Platelet-Derived Growth FactorRNA, ViralViral Structural ProteinsConceptsMouse C127 cellsE5 proteinC127 cellsE5 genePlatelet-derived growth factor beta receptorPDGF receptorBovine papillomavirus type 1 E5 proteinConstitutive tyrosine phosphorylationDNA synthesisGrowth factor beta receptorBovine papillomavirus type 1Platelet-derived growth factor receptorTransformation of fibroblastsPapillomavirus type 1Sequence similarityGrowth factor receptorTyrosine phosphorylationBiological activityShort regionFoci formationProteinFactor receptorReceptor formsB chainGrowth transformation
1990
Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity
Riese D, Settleman J, Neary K, DiMaio D. Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity. Journal Of Virology 1990, 64: 944-949. PMID: 2153255, PMCID: PMC249196, DOI: 10.1128/jvi.64.2.944-949.1990.Peer-Reviewed Original ResearchConceptsRepressor proteinWild-type copy numberBovine papillomavirus type 1 genomeCopy numberViral genomeTranscriptional repressor proteinTransient expression experimentsMouse C127 cellsMutant viral DNAViral DNAType 1 genomeNumber phenotypeRepressor activityLow copy numberMethionine codonInitiation codonExpression experimentsC127 cellsGenomeCV1 cellsRepressor mutantsFoci formationColony formationCodonProtein
1989
Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids
Horwitz B, Weinstat D, DiMaio D. Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids. Journal Of Virology 1989, 63: 4515-4519. PMID: 2552136, PMCID: PMC251082, DOI: 10.1128/jvi.63.11.4515-4519.1989.Peer-Reviewed Original ResearchConceptsE5 proteinAmino acidsWild-type E5 proteinBovine papillomavirus E5 proteinAmino acid sequence requirementsHydrophobic amino acid sequenceCarboxyl-terminal amino acidsMouse C127 cellsAmino acid sequenceBovine papillomavirus type 1Carboxyl-terminal portionWild-type onesHydrophobic amino acidsPapillomavirus type 1Hydrophobic sequenceDifferent amino acidsAcid sequenceC127 cellsSequence requirementsE5 geneCell transformationFoci formationSubstitution mutationsCell membraneProteinStructure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product
Horwitz B, Settleman J, Prakash S, DiMaio D. Structure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product. Current Topics In Microbiology And Immunology 1989, 144: 143-151. PMID: 2551579, DOI: 10.1007/978-3-642-74578-2_18.Peer-Reviewed Original ResearchConceptsE5 geneFoci formationBovine papillomavirus type 1 DNACell focus formationBPV geneCodon resultsC127 cellsMouse cellsTumorigenic transformationProtein productsK polypeptideGenesViral mutantsBiochemical analysisEfficient transformationCellsMutantsPolypeptideDNARegulationExpressionDownstreamActivityTranslationFormation
1988
Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus.
Settleman J, DiMaio D. Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 9007-9011. PMID: 2848252, PMCID: PMC282651, DOI: 10.1073/pnas.85.23.9007.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1 E2Simian virus 40 early regionTransactivation of genesCultured mammalian cellsHybrid viral genomesSimian virus 40 virionsMouse C127 cellsSimian virus 40 large tumor antigenBovine papillomavirus type 1Large tumor antigenPapillomavirus type 1E5 mutantsAcute cellular responseRegulatory genesMammalian cellsControl regionEfficient transactivationForeign genesReporter geneType 1 geneWild-type virusC127 cellsE5 geneTumorigenic transformationCellular responsesBovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene
Prakash S, Horwitz B, Zibello T, Settleman J, DiMaio D. Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene. Journal Of Virology 1988, 62: 3608-3613. PMID: 2843663, PMCID: PMC253501, DOI: 10.1128/jvi.62.10.3608-3613.1988.Peer-Reviewed Original ResearchAcetyltransferasesAmino Acid SequenceAnimalsBase SequenceBovine papillomavirus 1Cell LineCell Transformation, ViralChloramphenicol O-AcetyltransferaseDNA-Binding ProteinsGene Expression RegulationGenes, ViralMolecular Sequence DataMutationOncogene Proteins, ViralOncogenesPapillomaviridaePlasmidsPrecipitin TestsPromoter Regions, GeneticTransfectionViral ProteinsBovine papillomavirus mutant temperature sensitive for transformation, replication and transactivation.
DiMaio D, Settleman J. Bovine papillomavirus mutant temperature sensitive for transformation, replication and transactivation. The EMBO Journal 1988, 7: 1197-1204. PMID: 2841117, PMCID: PMC454456, DOI: 10.1002/j.1460-2075.1988.tb02931.x.Peer-Reviewed Original ResearchConceptsMutant viral DNANormal viral DNA replicationMajor viral regulatory proteinLack of mutantsN-terminal halfTemperature-sensitive defectMouse C127 cellsViral DNA replicationViral regulatory proteinsStrong genetic evidenceViral DNAPleiotropic phenotypesGenetic experimentsMost cell linesDNA replicationGene activityGenetic evidenceReplication defectRegulatory proteinsExtrachromosomal plasmidsMutant temperatureC127 cellsTemperature shiftGenetic analysisViral biological activities
1987
Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein.
Burkhardt A, DiMaio D, Schlegel R. Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein. The EMBO Journal 1987, 6: 2381-2385. PMID: 2822390, PMCID: PMC553643, DOI: 10.1002/j.1460-2075.1987.tb02515.x.Peer-Reviewed Original ResearchConceptsFirst methionine codonMethionine codonE5 proteinCellular membranesAmino-terminal mutationsFrameshift mutationBovine papillomavirus E5Mutant viral genomesCellular transformationInitiation codonHydrophobic polypeptidesBPV genomeSmall polypeptidesCell transformationCodonViral genomeE5 ORFProteinBovine papillomavirusGenetic alterationsMutationsPolypeptideForm dimersGenomeFrame mutationsMutational analysis of open reading frame E4 of bovine papillomavirus type 1
Neary K, Horwitz B, DiMaio D. Mutational analysis of open reading frame E4 of bovine papillomavirus type 1. Journal Of Virology 1987, 61: 1248-1252. PMID: 3029420, PMCID: PMC254088, DOI: 10.1128/jvi.61.4.1248-1252.1987.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1Papillomavirus type 1E4 proteinLate gene expressionMouse C127 cellsAmino acid sequenceAcid sequenceExtrachromosomal plasmidsBiological activityC127 cellsMutational analysisGene expressionFoci formationORF E2ORFProteinViral DNAMutationsSoft agaroseCellsMutantsType 1Papilloma formationDNAPlasmid
1986
Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity.
DiMaio D, Guralski D, Schiller J. Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1797-1801. PMID: 3006073, PMCID: PMC323171, DOI: 10.1073/pnas.83.6.1797.Peer-Reviewed Original ResearchConceptsMouse C127 cellsC127 cellsTransformation defectGenetic mapping experimentsCorrect reading frameStrong genetic evidenceBovine papillomavirus type 1Series of mutationsViral DNAPapillomavirus type 1Putative genesGenetic evidenceHydrophobic proteinsReading frameFrameshift mutantsPolypeptide domainsBovine papillomavirus DNAOncogenic transformationDifferent mutantsORFSecond mutationSequence changesMutantsMapping experimentsFrameshift mutationNonsense mutation in open reading frame E2 of bovine papillomavirus DNA
DiMaio D. Nonsense mutation in open reading frame E2 of bovine papillomavirus DNA. Journal Of Virology 1986, 57: 475-480. PMID: 3003380, PMCID: PMC252759, DOI: 10.1128/jvi.57.2.475-480.1986.Peer-Reviewed Original ResearchConceptsMutant viral DNAOpen reading frame E2C127 cellsBovine papillomavirus DNAViral DNAGenetic mapping experimentsNonsense mutationMouse C127 cellsSingle base substitution mutationsHost cell genomeFull-length viral DNAFocus-forming activityBase substitution mutationsWild-type DNAAmber mutationCell genomeOncogenic transformationViral ORFsSubstitution mutationsORF E2Mapping experimentsMutant DNATransformation assaysDNABovine papillomavirus
1982
Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes
DiMaio D, Nathans D. Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. Journal Of Molecular Biology 1982, 156: 531-548. PMID: 6288959, DOI: 10.1016/0022-2836(82)90265-0.Peer-Reviewed Original ResearchConceptsViral DNA replicationDNA replicationEarly gene transcriptionT antigenGene transcriptionGene expressionCold-sensitive defectSecond-site mutationsSegment of DNAT antigen functionLate protein synthesisSimian virus 40 mutantsSV40 early proteinsLarge T antigenT-antigen geneEarly gene expressionBase pair substitutionsRegulatory mutantsRegulatory segmentRegulatory regionsDefined deletionsViral regulatory regionViral genesMutantsDNA show
1980
Cold-sensitive regulatory mutants of simian virus 40
DiMaio D, Nathans D. Cold-sensitive regulatory mutants of simian virus 40. Journal Of Molecular Biology 1980, 140: 129-142. PMID: 6251230, DOI: 10.1016/0022-2836(80)90359-9.Peer-Reviewed Original ResearchConceptsRegulatory segmentBase substitutionsBglI siteWild-type plaquesProtein-coding sequencesProtein-nucleic acid interactionsCold-sensitive mutantsOrigin of replicationSet of mutantsSimian virus 40 mutantsDNA polymerase IBase substitution mutationsRNA processingRegulatory mutantsDNA replicationReplication originsViable mutantsSimian virus 40Small plaquesMutational alterationsWild-type virusPolymerase IMutantsRegulatory phenomenaSubstitution mutations