2012
Loop Diuretic and Ion-binding Residues Revealed by Scanning Mutagenesis of Transmembrane Helix 3 (TM3) of Na-K-Cl Cotransporter (NKCC1)*
Somasekharan S, Tanis J, Forbush B. Loop Diuretic and Ion-binding Residues Revealed by Scanning Mutagenesis of Transmembrane Helix 3 (TM3) of Na-K-Cl Cotransporter (NKCC1)*. Journal Of Biological Chemistry 2012, 287: 17308-17317. PMID: 22437837, PMCID: PMC3366785, DOI: 10.1074/jbc.m112.356014.Peer-Reviewed Original ResearchConceptsTransmembrane helix 3Na-K-Cl cotransporterTranslocation pathwayHelix 3Homology modelTryptophan-scanning mutagenesisMutation of residuesStructural homology modelEpithelial salt transportExtracellular gateCellular chloride homeostasisScanning mutagenesisOpen conformationIntracellular endPore residuesFunctional roleIon translocationTranslocation rateResiduesMutagenesisCentral roleChloride homeostasisMutationsPathwayLarge effect
1986
[3H]bumetanide binding to duck red cells. Correlation with inhibition of (Na + K + 2Cl) co-transport.
Haas M, Forbush B. [3H]bumetanide binding to duck red cells. Correlation with inhibition of (Na + K + 2Cl) co-transport. Journal Of Biological Chemistry 1986, 261: 8434-8441. PMID: 3013852, DOI: 10.1016/s0021-9258(19)83931-5.Peer-Reviewed Original ResearchConceptsDuck red cellsExtracellular sodiumRed cellsCo-transportDog kidney outer medullaBinding of [3H]bumetanideConcentration of extracellular sodiumIncreased extracellular sodiumPresence of norepinephrineCo-transport systemBumetanide inhibitionKidney outer medullaOuter medullaBiphasic effectInhibition of influxCell shrinkageHalf-maximalSaturable bindingBumetanideInhibit bindingCell typesSaturable mannerNorepinephrineInfluxBinding to membranes