Microhap Sequencing (mMHseq)

Microhaplotypes (microhaps, MH) are comprised of multiple single nucleotide polymorphisms (SNPs) that are located within 300 bases of genomic sequence. Improved tools are needed to facilitate broader application of microhaplotypes in a diverse range of populations and forensic settings. We have developed an assay for multiplex sequencing of 90 microhaplotypes (mMHseq) and open-source, web-based software to visualize haplotype phase data for each microhaplotype and individual.

• Validation of novel forensic DNA markers using multiplex microhaplotype sequencing
• The population genetics characteristics of a 90 locus panel of microhaplotypes.
• A multipurpose panel of microhaplotypes for use with STR markers in casework