Spatial transcriptomics reveals organized and distinct immune activation in cutaneous granulomatous disorders
Daccache J, Park E, Junejo M, Abdelghaffar M, Hwang E, Mohanty C, Singh C, Wang G, Wheeler J, Shields B, Nelson C, Wang Y, Damsky W. Spatial transcriptomics reveals organized and distinct immune activation in cutaneous granulomatous disorders. Journal Of Allergy And Clinical Immunology 2024, 154: 1216-1231. PMID: 39098508, PMCID: PMC11560686, DOI: 10.1016/j.jaci.2024.07.021.Peer-Reviewed Original ResearchCutaneous sarcoidosisNecrobiotic xanthogranulomaGranulomatous disorderGranuloma annulareNecrobiosis lipoidicaImmune activationMacrophage activationCutaneous granulomatous disordersPattern of immune activationType 2 immune activationActivation of Th1Pattern of Th1Alternative macrophage activationAlternative macrophage polarizationAlteration of extracellular matrixPatterns of macrophage activationCases of CSPredominant inflammationExtracellular matrix alterationsTh17 immunityT-helperTh2 polarizationIL-32Histological changesChemokine signalingGranuloma Annulare Exhibits Mixed Immune and Macrophage Polarization Profiles with Spatial Transcriptomics
Mohanty C, Singh C, Daccache J, Damsky W, Kendziorski C, Yan D, Prasad A, Zhang D, Keenan T, Drolet B, Ahmad N, Shields B. Granuloma Annulare Exhibits Mixed Immune and Macrophage Polarization Profiles with Spatial Transcriptomics. Journal Of Investigative Dermatology 2024 PMID: 38844128, DOI: 10.1016/j.jid.2024.04.024.Peer-Reviewed Original ResearchGranuloma annulareGranulomatous inflammationFDA-approved therapiesInflammatory signaling cascadesImpact of GAIFN-gImmune cellsInterleukin-32Idiopathic conditionTh2 signalsAdaptive immunityInterstitial patternMacrophage activationGene expression changesMolecular driversQuality of lifeGenome-wide gene expression changesMacrophage polarizationGranulomaMacrophagesTissue remodelingInflammationTNF signalingLocal gene expression patternsSpatial transcriptomics