2022
In-situ high-resolution 3D imaging combined with proteomics and metabolomics reveals enlargement of subcellular architecture and enhancement of photosynthesis pathways in nuclear-irradiated Chlorella pyrenoidosa
Guo W, Feng L, Wang Z, Guo J, Park D, Carroll B, Zhang X, Liu J, Cheng J. In-situ high-resolution 3D imaging combined with proteomics and metabolomics reveals enlargement of subcellular architecture and enhancement of photosynthesis pathways in nuclear-irradiated Chlorella pyrenoidosa. Chemical Engineering Journal 2022, 430: 133037. DOI: 10.1016/j.cej.2021.133037.Peer-Reviewed Original ResearchPhotosynthetic efficiencySubcellular organellesChlorella pyrenoidosaHigh growth rateCryo-electron tomographyWild-type cellsPhotosynthesis pathwaySubcellular architectureCarbon fixationCryo-FIB millingChlorophyll synthesisMutantsGrowth rateMacroscopic traitsOrganellesSubcellular morphologyC. pyrenoidosaProteomicsBeam scanning electron microscopyIon beam scanning electron microscopyMetabolomics dataPyrenoidosaUnprecedented detailSuch modificationsChloroplasts
2020
The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching
Carroll BL, Nishikino T, Guo W, Zhu S, Kojima S, Homma M, Liu J. The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching. ELife 2020, 9: e61446. PMID: 32893817, PMCID: PMC7505661, DOI: 10.7554/elife.61446.Peer-Reviewed Original ResearchConceptsFlagellar motorRotational switchingBacterial flagellar motorLarge conformational rearrangementsCryo-electron tomographyMajor structural remodelingConformational remodelingRotor complexCytoplasmic ringConformational rearrangementsProtein compositionConformational changesC-ringNovel insightsFliNFlagellaMolecular architectureFLIMVibrioFliGRemodelingMutantsStructural remodelingMechanismCells