2024
Don’t be so naïve
Horsley V, Nassereddine A. Don’t be so naïve. ELife 2024, 13: e103292. PMID: 39453398, PMCID: PMC11509665, DOI: 10.7554/elife.103292.Peer-Reviewed Original Research
2023
Apoptosis recognition receptors regulate skin tissue repair in mice
Justynski O, Bridges K, Krause W, Forni M, Phan Q, Sandoval-Schaefer T, Carter K, King D, Hsia H, Gazes M, Vyce S, Driskell R, Miller-Jensen K, Horsley V. Apoptosis recognition receptors regulate skin tissue repair in mice. ELife 2023, 12: e86269. PMID: 38127424, PMCID: PMC10735221, DOI: 10.7554/elife.86269.Peer-Reviewed Original ResearchIL-6 trans-signaling in a humanized mouse model of scleroderma
Odell I, Agrawal K, Sefik E, Odell A, Caves E, Kirkiles-Smith N, Horsley V, Hinchcliff M, Pober J, Kluger Y, Flavell R. IL-6 trans-signaling in a humanized mouse model of scleroderma. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2306965120. PMID: 37669366, PMCID: PMC10500188, DOI: 10.1073/pnas.2306965120.Peer-Reviewed Original ResearchConceptsBone marrow-derived immune cellsIL-6Human hematopoietic stem cellsImmune cellsT cellsScleroderma skinSoluble IL-6 receptorCD8 T cellsHumanized mouse modelPathogenesis of sclerodermaMesenchymal cellsFibroblast-derived IL-6IL-6 receptorIL-6 signalingT cell activationHuman IL-6Human T cellsExpression of collagenFibrosis improvementPansclerotic morpheaHuman endothelial cellsHumanized miceReduced markersSkin graftsHuman CD4
2022
Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins
Tsai P, Cameron C, Forni M, Wasko R, Naughton B, Horsley V, Gerstein M, Schlieker C. Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins. Cell Reports 2022, 41: 111675. PMID: 36417855, PMCID: PMC9827541, DOI: 10.1016/j.celrep.2022.111675.Peer-Reviewed Original ResearchConceptsProtein turnoverCellular quality control systemNuclear membrane proteinsQuality control machineryDistinct cellular compartmentsNuclear envelope proteinsGenetic screenProtein homeostasisUbiquitin ligasesControl machineryMembrane proteinsCellular compartmentsEnzyme Ube2g2Quality control systemEndoplasmic reticulumHuman diseasesEfficient biosynthesisHRD1RNF5Disease variantsTMEM33Envelope proteinSubstrate levelsDisease etiologyModel substrateAdipocyte plasticity in tissue regeneration, repair, and disease
Horsley V. Adipocyte plasticity in tissue regeneration, repair, and disease. Current Opinion In Genetics & Development 2022, 76: 101968. PMID: 35988318, DOI: 10.1016/j.gde.2022.101968.Peer-Reviewed Original ResearchConceptsMammalian tissue repairTissue repairEssential regulatorAdipocyte plasticityFunction of adipocytesCritical regulatorLipid-filled cellsMultiple tissuesTissue functionRegenerative medicineAdipocytesSkeletal muscleBioactive productsRegulatorMammary glandTherapeutic avenuesFibrotic lesionsEndocrine functionTissue regenerationPlasticityWound healingContractile fibroblastsTissueRepairTumorigenesisSingle cell transcriptomic landscape of diabetic foot ulcers
Theocharidis G, Thomas BE, Sarkar D, Mumme HL, Pilcher WJR, Dwivedi B, Sandoval-Schaefer T, Sîrbulescu RF, Kafanas A, Mezghani I, Wang P, Lobao A, Vlachos IS, Dash B, Hsia HC, Horsley V, Bhasin SS, Veves A, Bhasin M. Single cell transcriptomic landscape of diabetic foot ulcers. Nature Communications 2022, 13: 181. PMID: 35013299, PMCID: PMC8748704, DOI: 10.1038/s41467-021-27801-8.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCell Adhesion MoleculesChitinase-3-Like Protein 1Diabetes MellitusDiabetic FootEndothelial CellsExome SequencingFibroblastsGene Expression RegulationHigh-Throughput Nucleotide SequencingHumansHypoxia-Inducible Factor 1, alpha SubunitKeratinocytesLeukocytesMacrophagesMatrix Metalloproteinase 1Matrix Metalloproteinase 11Matrix Metalloproteinase 3Single-Cell AnalysisSkinTranscriptomeWound HealingConceptsDiabetic foot ulcerationSpatial transcriptomicsSingle-cell transcriptomic landscapeSingle-cell RNA sequencingPeripheral blood mononuclear cellsBlood mononuclear cellsDiabetic foot ulcersM1 macrophage polarizationNovel therapeutic approachesTranscriptomic landscapeWound healing microenvironmentRNA sequencingDFU patientsDevastating complicationFoot ulcerationDFU healingFoot ulcersDFU treatmentMononuclear cellsM1 macrophagesM2 macrophagesMacrophage polarizationTherapeutic approachesSame patientHigh abundance
2021
Fibroblasts: Origins, definitions, and functions in health and disease
Plikus MV, Wang X, Sinha S, Forte E, Thompson SM, Herzog EL, Driskell RR, Rosenthal N, Biernaskie J, Horsley V. Fibroblasts: Origins, definitions, and functions in health and disease. Cell 2021, 184: 3852-3872. PMID: 34297930, PMCID: PMC8566693, DOI: 10.1016/j.cell.2021.06.024.Peer-Reviewed Original ResearchConceptsDiverse mesenchymal cellsComplex extracellular matrixCell fateCellular progenyTissue homeostasisCell cycleBiochemical cuesReversible plasticityExtracellular matrixPositional informationMesenchymal cellsSkeletal muscleTissue repairFibrotic disordersFibroblastsLineagesNicheProgenyHomeostasisPhenotypeOrgansFatePlasticityCellsFunctionResearch Techniques Made Simple: Scientific Communication using Twitter
Daneshjou R, Shmuylovich L, Grada A, Horsley V. Research Techniques Made Simple: Scientific Communication using Twitter. Journal Of Investigative Dermatology 2021, 141: 1615-1621.e1. PMID: 34167718, DOI: 10.1016/j.jid.2021.03.026.Peer-Reviewed Original Research
2020
Statement on Racial Equality
Glass D, Gonzalez GR, Horsley V, Linos E. Statement on Racial Equality. Journal Of Investigative Dermatology 2020, 140: 1485. PMID: 32709273, PMCID: PMC7839962, DOI: 10.1016/j.jid.2020.06.009.Peer-Reviewed Original ResearchSmall-scale demixing in confluent biological tissues
Sahu P, Sussman DM, Rübsam M, Mertz AF, Horsley V, Dufresne ER, Niessen CM, Marchetti MC, Manning ML, Schwarz JM. Small-scale demixing in confluent biological tissues. Soft Matter 2020, 16: 3325-3337. PMID: 32196025, DOI: 10.1039/c9sm01084j.Peer-Reviewed Original ResearchSkin in the Game: Stem Cells in Repair, Cancer, and Homeostasis
Horsley V. Skin in the Game: Stem Cells in Repair, Cancer, and Homeostasis. Cell 2020, 181: 492-494. PMID: 32234524, DOI: 10.1016/j.cell.2020.03.019.Peer-Reviewed Original ResearchRegulated in Development and DNA Damage Responses 1 Prevents Dermal Adipocyte Differentiation and Is Required for Hair Cycle–Dependent Dermal Adipose Expansion
Rivera-Gonzalez GC, Klopot A, Sabin K, Baida G, Horsley V, Budunova I. Regulated in Development and DNA Damage Responses 1 Prevents Dermal Adipocyte Differentiation and Is Required for Hair Cycle–Dependent Dermal Adipose Expansion. Journal Of Investigative Dermatology 2020, 140: 1698-1705.e1. PMID: 32032578, PMCID: PMC7398827, DOI: 10.1016/j.jid.2019.12.033.Peer-Reviewed Original ResearchConceptsWhite adipose tissueAdipocyte precursor cellsAdipose tissueProtein kinase B signalingDNA damage response 1Loss of REDD1Precursor cellsProtein kinase BAdipogenic marker expressionKinase B signalingHigher lipid accumulationInguinal subcutaneous white adipose tissueGonadal white adipose tissueInterscapular brown adipose tissueSubcutaneous white adipose tissueWhite adipose tissue expansionNegative regulatorPostnatal day 18Wild-type miceAdipose tissue expansionKinase BRegulated developmentBrown adipose tissueHair growth cycleResponse 1
2019
Thin Skinned: Aged Adipocyte Atrophy Impacts Innate Immunity
Wasko RR, Horsley V. Thin Skinned: Aged Adipocyte Atrophy Impacts Innate Immunity. Trends In Immunology 2019, 40: 175-177. PMID: 30713009, DOI: 10.1016/j.it.2019.01.009.Peer-Reviewed Original Research
2018
Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation
Zwick RK, Rudolph MC, Shook BA, Holtrup B, Roth E, Lei V, Van Keymeulen A, Seewaldt V, Kwei S, Wysolmerski J, Rodeheffer MS, Horsley V. Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation. Nature Communications 2018, 9: 3592. PMID: 30181538, PMCID: PMC6123393, DOI: 10.1038/s41467-018-05911-0.Peer-Reviewed Original ResearchConceptsMouse mammary glandMilk-producing epithelial cellsTissue-specific rolesMammary glandAdipose growthLipid dynamicsGenetic tracingPhysiological examplesFunctional implicationsCellular mechanismsAdipocyte hypertrophyMature adipocytesEssential roleVivo analysisTissue functionMammary epitheliumAdipocytesEpithelial cellsAdipocyte precursorsSubsequent involutionMilk lipidsPrimary mechanismMechanismLipidomicsMilk fat productionAnatomical, Physiological, and Functional Diversity of Adipose Tissue
Zwick RK, Guerrero-Juarez CF, Horsley V, Plikus MV. Anatomical, Physiological, and Functional Diversity of Adipose Tissue. Cell Metabolism 2018, 27: 68-83. PMID: 29320711, PMCID: PMC6050204, DOI: 10.1016/j.cmet.2017.12.002.Peer-Reviewed Original ResearchConceptsStem cell quiescenceInnate immune barrierNon-traditional functionsFunctional diversityEpithelial stem cell quiescenceMammary glandCell quiescenceOrgan regenerationAdipocyte progenitorsHair folliclesNeighboring cellsMetabolic functionsRange of cancersNovel therapeutic approachesAdipose tissue depotsBacterial invasionAdipocytesProgenitorsAutoimmune disordersImmune barrierTherapeutic approachesClose associationAdipose tissueBone marrowTissue depots
2017
Tregs Expand the Skin Stem Cell Niche
Horsley V, Naik S. Tregs Expand the Skin Stem Cell Niche. Developmental Cell 2017, 41: 455-456. PMID: 28586641, DOI: 10.1016/j.devcel.2017.05.020.Peer-Reviewed Original ResearchRepeal and Replace: Adipocyte Regeneration in Wound Repair
Horsley V, Watt F. Repeal and Replace: Adipocyte Regeneration in Wound Repair. Cell Stem Cell 2017, 20: 424-426. PMID: 28388424, DOI: 10.1016/j.stem.2017.03.015.Peer-Reviewed Original Research
2016
The Role of Adipocytes in Tissue Regeneration and Stem Cell Niches
Shook B, Rivera Gonzalez G, Ebmeier S, Grisotti G, Zwick R, Horsley V. The Role of Adipocytes in Tissue Regeneration and Stem Cell Niches. Annual Review Of Cell And Developmental Biology 2016, 32: 1-23. PMID: 27146311, PMCID: PMC5157158, DOI: 10.1146/annurev-cellbio-111315-125426.Peer-Reviewed Original ResearchConceptsStem cell nicheFunction of WATWhite adipose tissueTissue homeostasisCell nicheMetabolic regulationNovel roleMetabolic physiologyMajor regulatorMature adipocytesImmune tissuesVivo regulationEssential roleRole of adipocytesHomeostasisWhite adipocytesAdipocytesTissue regenerationRegulationEndocrine homeostasisRegenerationTissueNicheRegulatorRoleMontagna Symposium 2015: Harnessing Stem Cells to Reveal Novel Skin Biology and Disease Treatments
Horsley V, Kulesz-Martin M, Wang XJ. Montagna Symposium 2015: Harnessing Stem Cells to Reveal Novel Skin Biology and Disease Treatments. Journal Of Investigative Dermatology 2016, 136: 893-896. PMID: 27107373, PMCID: PMC5572212, DOI: 10.1016/j.jid.2016.01.022.Peer-Reviewed Original Research
2015
Origin of fibrosing cells in systemic sclerosis
Ebmeier S, Horsley V. Origin of fibrosing cells in systemic sclerosis. Current Opinion In Rheumatology 2015, 27: 555-562. PMID: 26352735, PMCID: PMC4639394, DOI: 10.1097/bor.0000000000000217.Peer-Reviewed Original ResearchConceptsSystemic sclerosisEndothelial cellsInjury-induced fibrosisEpithelial cellsProgressive fibrosisAutoimmune diseasesTreatment optionsEffective therapyInjury modelFibrosis modelChronic natureAnimal modelsTissue stromaSclerosisCell originFibrosisUnknown originVariety of tissuesCellular originRecent evidenceFibrocytesDiseasePericytesSpecific organsAdipocytes