2020
An in situ collagen‐HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC‐derived vascular smooth muscle cells
Dash BC, Duan K, Xing H, Kyriakides TR, Hsia HC. An in situ collagen‐HA hydrogel system promotes survival and preserves the proangiogenic secretion of hiPSC‐derived vascular smooth muscle cells. Biotechnology And Bioengineering 2020, 117: 3912-3923. PMID: 32770746, DOI: 10.1002/bit.27530.Peer-Reviewed Original Research
2019
Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29
Calabro NE, Barrett A, Chamorro-Jorganes A, Tam S, Kristofik NJ, Xing H, Loye AM, Sessa WC, Hansen K, Kyriakides TR. Thrombospondin-2 regulates extracellular matrix production, LOX levels, and cross-linking via downregulation of miR-29. Matrix Biology 2019, 82: 71-85. PMID: 30876926, PMCID: PMC6710120, DOI: 10.1016/j.matbio.2019.03.002.Peer-Reviewed Original ResearchConceptsECM protein productionProtein productionLysyl oxidaseThrombospondin-2MiR-29Matricellular proteinQuantitative proteomics approachLevels of LOXExtracellular matrix productionProteomic approachECM proteinsMajor regulatorECM homeostasisExtracellular matrixMatrix productionProteinCollagen fibrillogenesisMiR-29 expressionFibrillar collagenImportant modulatorDependent processesLOX levelsRegulatorProductionHomeostasis
2017
A hidden structural vulnerability in the thrombospondin-2 deficient aorta increases the propensity to intramural delamination
Bellini C, Kristofik NJ, Bersi MR, Kyriakides TR, Humphrey JD. A hidden structural vulnerability in the thrombospondin-2 deficient aorta increases the propensity to intramural delamination. Journal Of The Mechanical Behavior Of Biomedical Materials 2017, 71: 397-406. PMID: 28412645, PMCID: PMC8344363, DOI: 10.1016/j.jmbbm.2017.01.045.Peer-Reviewed Original Research
2016
Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice
Kristofik N, Calabro NE, Tian W, Meng A, MacLauchlan S, Wang Y, Breuer CK, Tellides G, Niklason LE, Kyriakides TR. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 2016, 128: 1642-1650. PMID: 27471233, PMCID: PMC5034742, DOI: 10.1182/blood-2016-03-702845.Peer-Reviewed Original Research
2014
Engineering Cellular Response Using Nanopatterned Bulk Metallic Glass
Padmanabhan J, Kinser ER, Stalter MA, Duncan-Lewis C, Balestrini JL, Sawyer AJ, Schroers J, Kyriakides TR. Engineering Cellular Response Using Nanopatterned Bulk Metallic Glass. ACS Nano 2014, 8: 4366-4375. PMID: 24724817, PMCID: PMC4046793, DOI: 10.1021/nn501874q.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiocompatible MaterialsBiomechanical PhenomenaCell AdhesionCell SurvivalCollagenCytoskeletonFibroblastsFibronectinsForeign-Body ReactionGlassGTP PhosphohydrolasesHuman Umbilical Vein Endothelial CellsHumansMetalsMiceMicroscopy, FluorescenceNanostructuresNanotechnologyNIH 3T3 CellsProtein EngineeringRhoA GTP-Binding ProteinConceptsBulk metallic glassCellular responsesFeature sizeMetallic glassesFocal adhesion densityCell traction forcesCytoskeletal remodelingFIB-SEM techniqueSubstrate stiffnessBiomechanical cuesThermoplastic formingMolecular pathwaysCell typesVersatile fabricationNondimensional analysisSubstrate nanotopographyCellular morphologyCell morphologyBiocompatible materialsCell functionForeign body responseNanoscale precisionCell sizeTissue repairFibroblasts
2001
Regulation of Angiogenesis and Matrix Remodeling by Localized, Matrix-Mediated Antisense Gene Delivery
Kyriakides T, Hartzel T, Huynh G, Bornstein P. Regulation of Angiogenesis and Matrix Remodeling by Localized, Matrix-Mediated Antisense Gene Delivery. Molecular Therapy 2001, 3: 842-849. PMID: 11407897, DOI: 10.1006/mthe.2001.0336.Peer-Reviewed Original Research
2000
Thrombospondin 2 Modulates Collagen Fibrillogenesis and Angiogenesis
Bornstein P, Kyriakides T, Yang Z, Armstrong L, Birk D. Thrombospondin 2 Modulates Collagen Fibrillogenesis and Angiogenesis. Journal Of Investigative Dermatology Symposium Proceedings 2000, 5: 61-66. PMID: 11147677, DOI: 10.1046/j.1087-0024.2000.00005.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCollagenHumansMiceMice, KnockoutNeovascularization, PhysiologicSkinThrombospondinsConceptsVascular densityTSP2-null miceMatrix metalloproteinase-2 productionMetalloproteinase-2 productionConnective tissue abnormalitiesFlexor muscle tendonsExcisional skin woundsPolyvinyl alcohol spongesSilicone rubber discsAbnormal collagen fibrilsMuscle tendonSubcutaneous tissueNull miceTissue abnormalitiesDistinct abnormalitiesEndothelial cellsThrombospondin-2Matricellular proteinAlcohol spongesTHBS2 geneCell functionCell-matrix interactionsMiceSkin woundsMode of action
1998
Mice That Lack Thrombospondin 2 Display Connective Tissue Abnormalities That Are Associated with Disordered Collagen Fibrillogenesis, an Increased Vascular Density, and a Bleeding Diathesis
Kyriakides T, Zhu Y, Smith L, Bain S, Yang Z, Lin M, Danielson K, Iozzo R, LaMarca M, McKinney C, Ginns E, Bornstein P. Mice That Lack Thrombospondin 2 Display Connective Tissue Abnormalities That Are Associated with Disordered Collagen Fibrillogenesis, an Increased Vascular Density, and a Bleeding Diathesis. Journal Of Cell Biology 1998, 140: 419-430. PMID: 9442117, PMCID: PMC2132586, DOI: 10.1083/jcb.140.2.419.Peer-Reviewed Original ResearchConceptsTSP2-null miceEmbryonic stem cellsCollagen fibrillogenesisGenetic disorder resultsCell surface propertiesHomologous recombinationExtracellular proteinsMutant animalsBlastocyst injectionAppropriate breedingStructural roleMendelian frequencyMouse tissuesMesenchymal cellsStem cellsUnusual phenotypeCell functionMutant miceThrombospondin-2TSP2Von Willebrand factorSkin fibroblastsConnective tissue abnormalitiesLarge fibrilsWillebrand factor