2021
YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion
Feliciano D, Ott CM, Espinosa-Medina I, Weigel AV, Benedetti L, Milano KM, Tang Z, Lee T, Kliman HJ, Guller SM, Lippincott-Schwartz J. YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion. Nature Communications 2021, 12: 4502. PMID: 34301937, PMCID: PMC8302681, DOI: 10.1038/s41467-021-24708-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAMP-Activated Protein KinasesAnimalsBiological TransportCell FusionCell LineCell Line, TumorCell MembraneCell NucleusCells, CulturedGiant CellsHEK293 CellsHumansMembrane GlycoproteinsMiceRNA-SeqSignal TransductionTranscription FactorsTranscription, GeneticViral Envelope ProteinsYAP-Signaling ProteinsConceptsCell cycle arrestCell fusionNew cellular statesPlasma membrane surface areaRNA-seq analysisCell fusion systemTranscriptional programsNutrient stressCellular statesTranscriptional changesNuclear effluxCytoplasmic glucoseExtrinsic cuesUndifferentiated cellsGlucose transporterFused cellsMechanistic insightsMembrane surface areaNew functionsEndocytosisYAP1 inhibitionEnergetic stateSyncytiaCellsVSV
2020
Acetaminophen Attenuates invasion and alters the expression of extracellular matrix enzymes and vascular factors in human first trimester trophoblast cells
Burman A, Garcia-Milian R, Wood M, DeWitt NA, Vasiliou V, Guller S, Abrahams VM, Whirledge S. Acetaminophen Attenuates invasion and alters the expression of extracellular matrix enzymes and vascular factors in human first trimester trophoblast cells. Placenta 2020, 104: 146-160. PMID: 33348283, DOI: 10.1016/j.placenta.2020.12.002.Peer-Reviewed Original ResearchConceptsFirst trimester trophoblast cellsHuman first trimester trophoblast cellsPrenatal acetaminophen useTrophoblast cellsAcetaminophen useTrophoblast functionsAcetaminophen exposureFetal developmentHTR-8/SVneo cellsPrenatal acetaminophen exposureAnti-inflammatory drug aspirinRecent epidemiological studiesPlacental cell typesMatrix metalloproteinases 2Expression of angiogenesisExtracellular matrix enzymesPrenatal acetaminophenCommon medicationsVascular factorsMaternal healthPlacental functionEpidemiological associationLong-term effectsCyclooxygenase-1Epidemiological studiesTrophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function
Zhang Y, Aldo P, You Y, Ding J, Kaislasuo J, Petersen JF, Lokkegaard E, Peng G, Paidas MJ, Simpson S, Pal L, Guller S, Liu H, Liao AH, Mor G. Trophoblast-secreted soluble-PD-L1 modulates macrophage polarization and function. Journal Of Leukocyte Biology 2020, 108: 983-998. PMID: 32386458, PMCID: PMC8190653, DOI: 10.1002/jlb.1a0420-012rr.Peer-Reviewed Original ResearchConceptsSoluble PD-L1Macrophage polarizationTrophoblast cellsCell death ligand 1PD-L1/PDPD-1 blockageDeath ligand 1Macrophage differentiationModulates macrophage polarizationExpression/secretionDecidual macrophagesHealthy pregnancyPD-L1Pregnant womenTLR4 ligationM2 phenotypeExpression of typeFetal developmentLPS activationMacrophagesIFNPlacenta developmentLigand 1Constitutive productionGestationHuman Chorionic Gonadotropin modulates CXCL10 Expression through Histone Methylation in human decidua
Silasi M, You Y, Simpson S, Kaislasuo J, Pal L, Guller S, Peng G, Ramhorst R, Grasso E, Etemad S, Durosier S, Aldo P, Mor G. Human Chorionic Gonadotropin modulates CXCL10 Expression through Histone Methylation in human decidua. Scientific Reports 2020, 10: 5785. PMID: 32238853, PMCID: PMC7113245, DOI: 10.1038/s41598-020-62593-9.Peer-Reviewed Original ResearchConceptsHuman chorionic gonadotropinCXCL10 expressionChorionic gonadotropinPotent immune-modulatory effectsImmune system undergoesImmune modulatory effectsEndometrial stromal cellsImmune cell recruitmentMaternal-fetal interfaceImmune regulatory functionsProcess of implantationCD8 cellsCytokine profilePregnancy progressesFetal interfaceDecidual samplesImmune populationsImmune factorsHuman deciduaT cellsDecidual cellsCell recruitmentModulatory effectsEarly hormoneStromal cells
2019
In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines.
Kisanga EP, Tang Z, Guller S, Whirledge S. In Vitro Assays to Evaluate the Migration, Invasion, and Proliferation of Immortalized Human First-trimester Trophoblast Cell Lines. Journal Of Visualized Experiments 2019 PMID: 30907868, DOI: 10.3791/58942.Peer-Reviewed Original ResearchConceptsCell movementCell migrationHuman first trimester trophoblast cell lineFirst trimester trophoblast cell lineIntrauterine growth restrictionCell linesTrophoblast cell linePregnancy disordersEarly pregnancyGrowth restrictionMaternal vasculatureTrophoblast migrationMigratory capacityPlacental developmentPotential mechanismsCellular changesVitro assaysInvasionTrophoblastProliferationThorough examinationMigrationAssaysIndividual factorsPregnancy
2018
Excess glucose induce trophoblast inflammation and limit cell migration through HMGB1 activation of Toll‐Like receptor 4
Heim KR, Mulla MJ, Potter JA, Han CS, Guller S, Abrahams VM. Excess glucose induce trophoblast inflammation and limit cell migration through HMGB1 activation of Toll‐Like receptor 4. American Journal Of Reproductive Immunology 2018, 80: e13044. PMID: 30175447, DOI: 10.1111/aji.13044.Peer-Reviewed Original ResearchConceptsToll-like receptor 4High mobility group box 1Damage-associated molecular patternsHMGB1 activationIL-1βIL-8Trophoblast responsesReceptor 4Human first trimester trophoblast cell lineFirst trimester trophoblast cell lineInflammatory IL-8Risk of preeclampsiaGroup box 1Uric acidIL-1β responseExcess glucoseTrophoblast cell lineCell migrationTrophoblast inflammationPregnancy outcomesTLR4 antagonistLPS-RSIL-6SFlt-1HMGB1 inhibitorGlucocorticoid signaling regulates cell invasion and migration in the human first‐trimester trophoblast cell line Sw.71
Kisanga EP, Tang Z, Guller S, Whirledge S. Glucocorticoid signaling regulates cell invasion and migration in the human first‐trimester trophoblast cell line Sw.71. American Journal Of Reproductive Immunology 2018, 80: e12974. PMID: 29774963, DOI: 10.1111/aji.12974.Peer-Reviewed Original ResearchConceptsGene expression profilesSERPINE1 promoterPolymerase IITranscriptional regulationGlucocorticoid receptorCell movementTranscriptional profilesGlucocorticoid treatmentExpression profilesMicroarray analysisStress responseCell invasionPrimary mediatorPathogenesis of preeclampsiaCell proliferationEffects of glucocorticoidsExtravillous trophoblast functionsGlucocorticoid signalingSynthetic glucocorticoid dexamethasoneGenesTrophoblast functionsAdrenal axisInvasionTrophoblast tissueGlucocorticoid dexamethasone
2016
Inflammatory processes are specifically enhanced in endothelial cells by placental-derived TNF-α: Implications in preeclampsia (PE)
Shaw J, Tang Z, Schneider H, Saljé K, Hansson SR, Guller S. Inflammatory processes are specifically enhanced in endothelial cells by placental-derived TNF-α: Implications in preeclampsia (PE). Placenta 2016, 43: 1-8. PMID: 27324092, DOI: 10.1016/j.placenta.2016.04.015.Peer-Reviewed Original ResearchConceptsPro-inflammatory cytokine secretionMaternal perfusateCytokine secretionEndothelial dysfunctionActivation markersEndothelial cellsTNF-α blocking antibodyDual perfusion modelMaternal endothelial dysfunctionPro-inflammatory cytokinesMCP-1 secretionTNF-α actionEndothelial cell linePlacental pathophysiologyEndothelial activationIL-6IL-8Maternal endotheliumInflammatory processDual perfusionMaternal circulationBlocking antibodiesPreeclampsiaTNFPerfusion model
2014
Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages
Aldo PB, Racicot K, Craviero V, Guller S, Romero R, Mor G. Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages. American Journal Of Reproductive Immunology 2014, 72: 270-284. PMID: 24995492, PMCID: PMC4230492, DOI: 10.1111/aji.12288.Peer-Reviewed Original ResearchConceptsUnique macrophage phenotypeMacrophage phenotypeMonocyte differentiationFirst trimester trophoblast cellsPeripheral blood monocytesPhenotype of macrophagesCD14 surface expressionNormal deciduaHealthy pregnancyIL-10Chemokine productionEarly pregnancyIP-10Monocyte exposureIL-1BBlood monocytesDecidual phenotypePhagocytic capacityTrophoblast cellsFlow cytometryMorphologic changesMacrophagesMonocytesSurface expressionPlacenta development
2013
Effect of Culture Conditions on the Phenotype of THP‐1 Monocyte Cell Line
Aldo PB, Craveiro V, Guller S, Mor G. Effect of Culture Conditions on the Phenotype of THP‐1 Monocyte Cell Line. American Journal Of Reproductive Immunology 2013, 70: 80-86. PMID: 23621670, PMCID: PMC3703650, DOI: 10.1111/aji.12129.Peer-Reviewed Original Research
2012
Cutting‐Edge Report: TLR10 Plays a Role in Mediating Bacterial Peptidoglycan‐Induced Trophoblast Apoptosis
Mulla MJ, Myrtolli K, Tadesse S, Stanwood NL, Gariepy A, Guller S, Norwitz ER, Abrahams VM. Cutting‐Edge Report: TLR10 Plays a Role in Mediating Bacterial Peptidoglycan‐Induced Trophoblast Apoptosis. American Journal Of Reproductive Immunology 2012, 69: 449-453. PMID: 23279063, PMCID: PMC3620725, DOI: 10.1111/aji.12065.Peer-Reviewed Original ResearchConceptsIL-6 secretionTrophoblast apoptosisTLR2 coreceptorsHuman trophoblast cell lineIntrauterine bacterial infectionFirst-trimester human trophoblast cell lineThird-trimester human placental tissueHuman placental tissueTrophoblast cell linePositive bacterial componentsTLR10 expressionPreterm laborThird trimesterCaspase-3 activityIL-6TLR2 activationPlacental tissuePlacental apoptosisBacterial infectionsTLR10Bacterial componentsBacterial peptidoglycanTrophoblastCell linesApoptosis
2009
The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71
Straszewski-Chavez SL, Abrahams VM, Alvero AB, Aldo PB, Ma Y, Guller S, Romero R, Mor G. The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71. Placenta 2009, 30: 939-948. PMID: 19766308, PMCID: PMC2784169, DOI: 10.1016/j.placenta.2009.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticApoptosisCell LineChorionic GonadotropinCytokinesFemaleFibronectinsHistocompatibility Antigens Class IHLA AntigensHLA-G AntigensHumansIntercellular Signaling Peptides and ProteinsKeratin-7Leukocyte Common AntigensPregnancyPregnancy Trimester, FirstTelomeraseThy-1 AntigensTrophoblastsVimentinConceptsFirst trimester trophoblast cell lineSwan 71 cellsTrophoblast cell lineFirst trimester trophoblast cellsFibroblast-specific antigenHuman chorionic gonadotrophinPrimary trophoblast cellsTrophoblast cellsHuman telomerase reverse transcriptaseFetal fibronectinCell linesCD90/ThySwan 71Growth factor profileTNF-alpha-induced apoptosisChorionic gonadotrophinCytokeratin 7Specific antigenTelomerase reverse transcriptaseNormal placentaFactor profileKaryotypic abnormalitiesPatient samplesPrimary human cellsAdequate cell numbers
2007
Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line
Meade ES, Ma YY, Guller S. Role of Hypoxia-Inducible Transcription Factors 1α and 2α in the Regulation of Plasminogen Activator Inhibitor-1 Expression in a Human Trophoblast Cell Line. Placenta 2007, 28: 1012-1019. PMID: 17570486, PMCID: PMC2001228, DOI: 10.1016/j.placenta.2007.04.005.Peer-Reviewed Original ResearchConceptsPAI-1 expressionHTR-8/SVneo cellsPlasminogen activator inhibitorTrophoblast cell linePAI-1HIF-1alphaHIF-2alphaFirst trimester extravillous trophoblast cell lineHuman trophoblast cell linePlasmin-dependent fibrinolysisExtravillous trophoblast cell linePlasminogen activator inhibitor-1 expressionHypoxia-inducible transcription factor-1αHypoxic conditionsPAI-1 protein levelsCell linesPAI-1 mRNAEarly pregnancyExtracellular matrix degradationHypoxia-inducible transcription factorsQuantitative real-time PCRReal-time PCRActivator inhibitorFactor-1αWestern blottingMethod to Enhance Transfection Efficiency of Cell Lines and Placental Fibroblasts
Zhang M, Guller S, Huang Y. Method to Enhance Transfection Efficiency of Cell Lines and Placental Fibroblasts. Placenta 2007, 28: 779-782. PMID: 17355893, DOI: 10.1016/j.placenta.2007.01.012.Peer-Reviewed Original Research
2006
First-trimester trophoblast cell model gene response to hypoxia
Koklanaris N, Nwachukwu JC, Huang SJ, Guller S, Karpisheva K, Garabedian M, Lee MJ. First-trimester trophoblast cell model gene response to hypoxia. American Journal Of Obstetrics And Gynecology 2006, 194: 687-693. PMID: 16522398, DOI: 10.1016/j.ajog.2006.01.067.Peer-Reviewed Original ResearchConceptsReverse transcriptase-polymerase chain reactionQuantitative reverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionPolymerase chain reactionHypoxia-responsive genesHTR-8/SVneoChain reactionCell linesTrophoblast cell linePregnancy outcomesEarly pregnancyMotility/migrationHypoxic environmentTotal RNAHypoxiaPlacentationMicroarray studiesGene responsesPreeclampsiaPregnancy