2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2018
A fine-tuned vector-parasite dialogue in tsetse's cardia determines peritrophic matrix integrity and trypanosome transmission success
Vigneron A, Aksoy E, Weiss BL, Bing X, Zhao X, Awuoche EO, O'Neill MB, Wu Y, Attardo GM, Aksoy S. A fine-tuned vector-parasite dialogue in tsetse's cardia determines peritrophic matrix integrity and trypanosome transmission success. PLOS Pathogens 2018, 14: e1006972. PMID: 29614112, PMCID: PMC5898766, DOI: 10.1371/journal.ppat.1006972.Peer-Reviewed Original Research
2014
Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis
Initiative I, Attardo G, Abila P, Auma J, Baumann A, Benoit J, Brelsfoard C, Ribeiro J, Cotton J, Pham D, Darby A, Van Den Abbeele J, Denlinger D, Field L, Nyanjom S, Gaunt M, Geiser D, Gomulski L, Haines L, Hansen I, Jones J, Kibet C, Kinyua J, Larkin D, Lehane M, Rio R, Macdonald S, Macharia R, Malacrida A, Marco H, Marucha K, Masiga D, Meuti M, Mireji P, Obiero G, Koekemoer J, Okoro C, Omedo I, Osamor V, Balyeidhusa A, Peyton J, Price D, Quail M, Ramphul U, Rawlings N, Riehle M, Robertson H, Sanders M, Scott M, Dashti Z, Snyder A, Srivastava T, Stanley E, Swain M, Hughes D, Tarone A, Taylor T, Telleria E, Thomas G, Walshe D, Wilson R, Winzerling J, Acosta-Serrano A, Aksoy S, Arensburger P, Aslett M, Bateta R, Benkahla A, Berriman M, Bourtzis K, Caers J, Caljon G, Christoffels A, Falchetto M, Friedrich M, Fu S, Gäde G, Githinji G, Gregory R, Hall N, Harkins G, Hattori M, Hertz-Fowler C, Hide W, Hu W, Imanishi T, Inoue N, Jonas M, Kawahara Y, Koffi M, Kruger A, Lawson D, Lehane S, Lehväslaiho H, Luiz T, Makgamathe M, Malele I, Manangwa O, Manga L, Megy K, Michalkova V, Mpondo F, Mramba F, Msangi A, Mulder N, Murilla G, Mwangi S, Okedi L, Ommeh S, Ooi C, Ouma J, Panji S, Ravel S, Rose C, Sakate R, Schoofs L, Scolari F, Sharma V, Sim C, Siwo G, Solano P, Stephens D, Suzuki Y, Sze S, Touré Y, Toyoda A, Tsiamis G, Tu Z, Wamalwa M, Wamwiri F, Wang J, Warren W, Watanabe J, Weiss B, Willis J, Wincker P, Zhang Q, Zhou J. Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis. Science 2014, 344: 380-386. PMID: 24763584, PMCID: PMC4077534, DOI: 10.1126/science.1249656.Peer-Reviewed Original ResearchConceptsGenome sequenceLactation-specific proteinsProtein-encoding genesBacterial genome sequencesPathogen recognition proteinsTsetse fliesMicrobial symbiosesTsetse biologyViviparous reproductionGenome dataRecognition proteinsSole vectorsChromosomal integrationDisease vectorsAfrican trypanosomiasisGenomeGenesFliesProteinSequenceMultiple aspectsHuman African trypanosomiasisImportant insightsMultiple discoveriesSymbioses
2000
A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans
Li S, Aksoy S. A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans. Gene 2000, 252: 83-93. PMID: 10903440, DOI: 10.1016/s0378-1119(00)00226-2.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DeaminaseAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernBlotting, SouthernDNADNA, ComplementaryFemaleGene Expression RegulationGene Expression Regulation, DevelopmentalGrowth SubstancesMaleMolecular Sequence DataRNA, MessengerSalivaSalivary GlandsSequence Analysis, DNASpecies SpecificityTissue DistributionTsetse FliesConceptsOpen reading frameFemale adult fliesDeduced protein sequenceSignal peptide characteristicDifferent fly tissuesFamily of genesFull-length cDNAAmino acid identityLarval developmental stagesRegulation of expressionN-terminal regionGrowth factorAmino acid residuesTissues of adultAnalysis of proteinsGenomic arrangementPutative proteinFly tissuesSarcophaga peregrinaAcid identityHydrophobic sequenceCDNA libraryReading frameAdult fliesProtein sequences
1998
Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans
Cappello M, Li S, Chen X, Li C, Harrison L, Narashimhan S, Beard C, Aksoy S. Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14290-14295. PMID: 9826693, PMCID: PMC24366, DOI: 10.1073/pnas.95.24.14290.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnticoagulantsAntithrombin ProteinsAntithrombinsBase SequenceBloodCloning, MolecularDigestive SystemDNA, ComplementaryEatingEscherichia coliFemaleGene Expression RegulationInsect ProteinsMaleMolecular Sequence DataPupaRecombinant ProteinsRNA, MessengerSalivary GlandsThrombinTranscription, GeneticTsetse FliesConceptsTsetse thrombin inhibitorPutative secretory signal peptideNative proteinGlossina morsitans morsitansFull-length cDNADegenerate oligonucleotide probeSecretory signal peptideSalivary gland cDNA libraryLack of homologyMorsitans morsitansUntranslated segmentSignal peptideInhibitor geneCDNA librarySingle copyTsetse genomeAdult tsetse fliesFamily of moleculesSegment codesEscherichia coliOptimal inhibitory activityOligonucleotide probesTsetse fliesSalivary glandsGut tissue