2020
Oral glutamine supplementation increases seizure severity in a rodent model of mesial temporal lobe epilepsy
Dhaher R, Chen EC, Perez E, Rapuano A, Sandhu MRS, Gruenbaum SE, Deshpande K, Dai F, Zaveri HP, Eid T. Oral glutamine supplementation increases seizure severity in a rodent model of mesial temporal lobe epilepsy. Nutritional Neuroscience 2020, 25: 64-69. PMID: 31900092, PMCID: PMC8970572, DOI: 10.1080/1028415x.2019.1708568.Peer-Reviewed Original ResearchConceptsMesial temporal lobe epilepsyOral glutamine supplementationSeverity of seizuresTemporal lobe epilepsyGlutamine supplementationSeizure severityLobe epilepsyRefractory mesial temporal lobe epilepsyMale Sprague-DawleySeizure frequencyConvulsive seizuresSeizure propagationEpilepsy modelSprague-DawleyEffective treatmentRodent modelsSeizuresSupplementationSeverityPotential roleEpilepsyFunction mutationsHippocampusGlutamine synthetaseDays
2018
Selective deletion of glutamine synthetase in the mouse cerebral cortex induces glial dysfunction and vascular impairment that precede epilepsy and neurodegeneration
Zhou Y, Dhaher R, Parent M, Hu QX, Hassel B, Yee SP, Hyder F, Gruenbaum SE, Eid T, Danbolt NC. Selective deletion of glutamine synthetase in the mouse cerebral cortex induces glial dysfunction and vascular impairment that precede epilepsy and neurodegeneration. Neurochemistry International 2018, 123: 22-33. PMID: 30053506, PMCID: PMC8261904, DOI: 10.1016/j.neuint.2018.07.009.Peer-Reviewed Original ResearchConceptsAbnormal glutamate metabolismMouse cerebral cortexCerebral blood vesselsNovel mouse modelPostnatal week threeWeeks of ageProgressive astrogliosisVascular impairmentSpontaneous seizuresCerebral cortexGlial dysfunctionNeurovascular couplingDeficient miceSclerotic areasMouse modelEpileptic patientsApparent malformationsProgressive neurodegenerationSelective deletionGlutamate-ammonia ligaseTissue levelsGlutamate metabolismBlood vesselsWeek threeNeurodegeneration