2003
Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis
Shepard K, Gerber A, Jambhekar A, Takizawa P, Brown P, Herschlag D, DeRisi J, Vale R. Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 11429-11434. PMID: 13679573, PMCID: PMC208774, DOI: 10.1073/pnas.2033246100.Peer-Reviewed Original ResearchConceptsDNA microarray analysisBud tipRNA transportMRNA localizationMicroarray analysisCell wall maintenanceCytoplasmic mRNA transportCytoplasmic mRNA localizationAsymmetric localizationMRNA transportYeast SaccharomycesRNA reportersProtein activityUntranslated regionCell asymmetryStress responseSecondary screenYeastMRNAProteinLocalizationShe2pMetazoansMyo4pShe3p
2002
The Khd1 protein, which has three KH RNA‐binding motifs, is required for proper localization of ASH1 mRNA in yeast
Irie K, Tadauchi T, Takizawa P, Vale R, Matsumoto K, Herskowitz I. The Khd1 protein, which has three KH RNA‐binding motifs, is required for proper localization of ASH1 mRNA in yeast. The EMBO Journal 2002, 21: 1158-1167. PMID: 11867544, PMCID: PMC125877, DOI: 10.1093/emboj/21.5.1158.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsBinding SitesCell PolarityCytoskeletonDeoxyribonucleases, Type II Site-SpecificDNA-Binding ProteinsMacromolecular SubstancesMolecular Motor ProteinsPhenotypeProtein BiosynthesisProtein Interaction MappingProto-Oncogene ProteinsRecombinant Fusion ProteinsRegulatory Sequences, Nucleic AcidRepressor ProteinsRibonucleoproteinsRNA, FungalRNA, MessengerRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence DeletionTranscription FactorsConceptsASH1 mRNADaughter cellsNovel RNA-binding proteinDaughter cell nucleiASH1 mRNA localizationRNA-binding proteinCo-immunoprecipitation studiesKH RNARNA localizationSpecific repressorActin cytoskeletonAsh1 proteinWidespread mechanismLocalization sequenceMRNA localizationProper localizationProtein synthesisCell nucleiKhd1Distal tipMyosin motorsHO expressionMRNAProteinRNA
2000
The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p
Takizawa P, Vale R. The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 5273-5278. PMID: 10792032, PMCID: PMC25818, DOI: 10.1073/pnas.080585897.Peer-Reviewed Original Research
1997
Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast
Takizawa P, Sil A, Swedlow J, Herskowitz I, Vale R. Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast. Nature 1997, 389: 90-93. PMID: 9288973, DOI: 10.1038/38015.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnaphaseBase SequenceBiological TransportCell DivisionCytoskeletonDNA-Binding ProteinsFungal ProteinsGene DeletionIn Situ Hybridization, FluorescenceMicroscopy, FluorescenceRepressor ProteinsRNA, FungalRNA, MessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsConceptsCell fate determinantsASH1 mRNACell fateDaughter budsActin cytoskeletonMating-type switchingMother-bud neckActin-dependent localizationMulticellular eukaryotesHigher eukaryotesRNA localizationCell cortexMRNA particlesCytoplasmic localizationAsymmetric distributionUntranslated regionActin filamentsMessenger RNADistal tipEukaryotesCytoskeletonMRNAYeastRNADaughter nuclei