2000
High-Efficiency, Long-Term Cardiac Expression of Foreign Genes in Living Mouse Embryos and Neonates
Christensen G, Minamisawa S, Gruber P, Wang Y, Chien K. High-Efficiency, Long-Term Cardiac Expression of Foreign Genes in Living Mouse Embryos and Neonates. Circulation 2000, 101: 178-184. PMID: 10637206, DOI: 10.1161/01.cir.101.2.178.Peer-Reviewed Original ResearchConceptsMouse myocardiumDirect intracardiac injectionAdult mouse myocardiumCardiac gene transferNeonatal miceIntracardiac injectionInfected myocytesMicrosurgical approachFunctional assessmentGene transfer strategiesCardiac expressionVentricular cavityVivo gene transferNormal physiological functionCardiac ventriclesGene transferMurine heartGene expressionPattern of expressionMyocardiumRecombinant adenovirusGene ablationAdenovirus vectorLong-term foreign gene expressionCardiac wall
1997
Developmental expression of the murine spliceosome‐associated protein mSAP49
Ruiz‐Lozano P, Doevendans P, Brown A, Gruber P, Chien K. Developmental expression of the murine spliceosome‐associated protein mSAP49. Developmental Dynamics 1997, 208: 482-490. PMID: 9097020, DOI: 10.1002/(sici)1097-0177(199704)208:4<482::aid-aja4>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsRNA recognition motifTissue-specific splicingBasic C-terminusAmino acid sequencePeak of expressionHuman spliceosomeSpliceosome componentsSitu hybridization analysisMouse homologueRecognition motifRNA stabilizationAcid sequenceCardiac developmentC-terminusMouse embryosN-terminusNorthern analysisEmbryonic tissuesDevelopmental expressionHybridization analysisDay 11.5Optic vesicleMRNA distributionDay 13.5Spliceosome