2007
Ontogeny of NHE8 in the rat proximal tubule
Becker AM, Zhang J, Goyal S, Dwarakanath V, Aronson PS, Moe OW, Baum M. Ontogeny of NHE8 in the rat proximal tubule. American Journal Of Physiology. Renal Physiology 2007, 293: f255-f261. PMID: 17429030, PMCID: PMC4119019, DOI: 10.1152/ajprenal.00400.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternDNA, ComplementaryElectrophoresis, Polyacrylamide GelFemaleImmunohistochemistryKidney Tubules, ProximalMicroscopy, FluorescenceMicrovilliPregnancyProteinsRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRNASodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsRat proximal tubuleProximal tubulesAdult ratsNHE3 expressionProximal tubule bicarbonate reabsorptionReal-time RT-PCRProton secretionNeonatal proximal tubuleNHE8 expressionApical membraneBicarbonate reabsorptionMaturational increaseMaturational changesNeonatesNHE8RT-PCRBrush border membrane vesiclesTubulesRatsCortical membrane proteinsSecretionPresent studyDevelopmental expressionAdultsBorder membrane vesiclesNHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact role
2005
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. American Journal Of Physiology. Renal Physiology 2005, 289: f249-f258. PMID: 15687252, DOI: 10.1152/ajprenal.00082.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAntibodies, MonoclonalAntibody SpecificityCell LineChlorocebus aethiopsCOS CellsCyclic AMP-Dependent Protein KinasesDopamineElectrophoresis, Polyacrylamide GelImmunohistochemistryKidneyMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceMicrovilliOpossumsPhosphorylationRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSubcellular FractionsTransfectionConceptsSerine 552Total NHE3Brush border membranePKA consensus sitesEffects of dopamineProximal tubule cellsWestern blot assaysOpossum kidney cellsBaseline phosphorylationPeptide antibodiesInhibition of NHE3Tubule cellsBlot assaysAntibodiesPhosphorylation of NHE3Phosphospecific antibodiesExchanger NHE3NHE3DopamineEndogenous NHE3Kidney cellsVivoPhospho-specific antibodiesTransfection studiesVitro
2004
Immunolocalization of NHE8 in rat kidney
Goyal S, Mentone S, Aronson PS. Immunolocalization of NHE8 in rat kidney. American Journal Of Physiology. Renal Physiology 2004, 288: f530-f538. PMID: 15522984, DOI: 10.1152/ajprenal.00229.2004.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityBiotinChlorocebus aethiopsCOS CellsElectrophoresis, Polyacrylamide GelFluorescent Antibody Technique, DirectHybridomasImmunohistochemistryKidneyKidney Tubules, ProximalMembranesMiceMice, KnockoutMicrovilliMolecular Sequence DataRatsRats, Sprague-DawleySodium-Hydrogen ExchangersThe Cl−/HCO3− exchanger pendrin in the rat kidney is regulated in response to chronic alterations in chloride balance
Quentin F, Chambrey R, Trinh-Trang-Tan MM, Fysekidis M, Cambillau M, Paillard M, Aronson PS, Eladari D. The Cl−/HCO3− exchanger pendrin in the rat kidney is regulated in response to chronic alterations in chloride balance. American Journal Of Physiology. Renal Physiology 2004, 287: f1179-f1188. PMID: 15292050, DOI: 10.1152/ajprenal.00211.2004.Peer-Reviewed Original ResearchConceptsChronic furosemide administrationBlood pressure regulationDiet-induced changesAcid-base statusNaCl dietFurosemide administrationChronic alterationsControl ratsRenal membrane fractionsChloride balanceChronic changesNaCl balanceExchanger pendrinSemiquantitative immunoblottingPressure regulationRat kidneyConsistent inverse relationshipPD expressionNaCl depletionType BProtein abundanceRatsPendrinInverse relationshipExpression
2001
Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron
Wang T, Hropot M, Aronson P, Giebisch G. Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron. American Journal Of Physiology. Renal Physiology 2001, 281: f1117-f1122. PMID: 11704563, DOI: 10.1152/ajprenal.2001.281.6.f1117.Peer-Reviewed Original ResearchConceptsLoop of HenleHOE 694Proximal tubulesAddition of ethylisopropylamilorideDistal convoluted tubuleBicarbonate reabsorptionConvoluted tubulesSitu microperfusionRat kidneySignificant inhibitionNHE isoformsHenlePredominant isoformTubulesNHE3Functional roleInhibitionNHE2Apical membraneIsoformsApical isoformKidneyMicroperfusionEthylisopropylamilorideNephronRole of sodium/hydrogen exchanger isoform NHE3 in fluid secretion and absorption in mouse and rat cholangiocytes
Mennone A, Biemesderfer D, Negoianu D, Yang C, Abbiati T, Schultheis P, Shull G, Aronson P, Boyer J. Role of sodium/hydrogen exchanger isoform NHE3 in fluid secretion and absorption in mouse and rat cholangiocytes. AJP Gastrointestinal And Liver Physiology 2001, 280: g247-g254. PMID: 11208547, DOI: 10.1152/ajpgi.2001.280.2.g247.Peer-Reviewed Original Research
2000
Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*
Biemesderfer D, DeGray B, Aronson P. Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*. Journal Of Biological Chemistry 2000, 276: 10161-10167. PMID: 11120742, DOI: 10.1074/jbc.m008098200.Peer-Reviewed Original ResearchAcridine OrangeAnimalsAntibodiesCarrier ProteinsCell MembraneCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelEndosomesFluorescent Antibody Technique, IndirectGoatsHeymann Nephritis Antigenic ComplexHydrogenHydrogen-Ion ConcentrationImmunoblottingImmunohistochemistryKidneyKidney TubulesMagnesiumMaleMembrane GlycoproteinsMicrofilament ProteinsMicroscopy, ElectronMicrovilliMitochondriaPrecipitin TestsProtein BindingProtein Structure, TertiaryRabbitsRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSucroseTime Factors
1998
Effect of metabolic acidosis on NaCl transport in the proximal tubule
Wang T, Egbert A, Aronson P, Giebisch G. Effect of metabolic acidosis on NaCl transport in the proximal tubule. American Journal Of Physiology 1998, 274: f1015-f1019. PMID: 9841491, DOI: 10.1152/ajprenal.1998.274.6.f1015.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionAcidosisAmmonium ChlorideAnimalsKidney Tubules, ProximalMaleRatsRats, Sprague-DawleySodium ChlorideConceptsMetabolic acidosisProximal tubulesNaCl reabsorptionRat proximal tubule segmentsAcidotic ratsNaCl transportNaCl absorptionLate proximal tubuleProximal tubule segmentsControl ratsCapillary perfusateBicarbonate absorptionNephron segmentsAcidosisTubule segmentsRatsRecent evidenceJclTubulesFormate exchangeReabsorptionDifferential regulation
1997
Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Nephron 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1Antibodies
1996
Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*
Wu M, Biemesderfer D, Giebisch G, Aronson P. Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*. Journal Of Biological Chemistry 1996, 271: 32749-32752. PMID: 8955109, DOI: 10.1074/jbc.271.51.32749.Peer-Reviewed Original ResearchConceptsMetabolic acidosisControl ratsBrush border membraneRenal brush border vesiclesNHE3 proteinBrush border vesiclesBorder membraneBrush borderNHE3 protein expressionRole of NHE3Western blot analysisNormal ratsAcidosisRatsAcidotic ratsRenal brush border membranePharmacologic inhibitorsProtein expressionExchange activityInhibitor sensitivityNHE3Renal brush borderBlot analysisMechanisms of stimulation of proximal tubule chloride transport by formate and oxalate
Wang T, Egbert AL, Abbiati T, Aronson PS, Giebisch G. Mechanisms of stimulation of proximal tubule chloride transport by formate and oxalate. American Journal Of Physiology 1996, 271: f446-f450. PMID: 8770178, DOI: 10.1152/ajprenal.1996.271.2.f446.Peer-Reviewed Original Research
1995
Stimulation of chloride transport by cAMP in rat proximal tubules
Wang T, Segal AS, Giebisch G, Aronson PS. Stimulation of chloride transport by cAMP in rat proximal tubules. American Journal Of Physiology 1995, 268: f204-f210. PMID: 7864157, DOI: 10.1152/ajprenal.1995.268.2.f204.Peer-Reviewed Original ResearchConceptsRat proximal tubuleProximal tubulesDibutyryl cAMPCapillary perfusateLate proximal tubuleProximal tubule cellsCl- channel blockerCl- channelsTranscellular Cl absorptionAddition of forskolinLuminal DIDSSensitive Cl- channelBasolateral membraneLuminal perfusateTubule cellsCAMP productionFluid absorptionCl absorptionMM ClPerfusateJclRecent evidenceLuminal membraneTubulesEffect of cAMP
1993
Effects of formate and oxalate on chloride absorption in rat distal tubule
Wang T, Agulian SK, Giebisch G, Aronson PS. Effects of formate and oxalate on chloride absorption in rat distal tubule. American Journal Of Physiology 1993, 264: f730-f736. PMID: 8386473, DOI: 10.1152/ajprenal.1993.264.4.f730.Peer-Reviewed Original Research