2011
High Throughput Determination of TGFβ1/SMAD3 Targets in A549 Lung Epithelial Cells
Zhang Y, Handley D, Kaplan T, Yu H, Bais AS, Richards T, Pandit KV, Zeng Q, Benos PV, Friedman N, Eickelberg O, Kaminski N. High Throughput Determination of TGFβ1/SMAD3 Targets in A549 Lung Epithelial Cells. PLOS ONE 2011, 6: e20319. PMID: 21625455, PMCID: PMC3098871, DOI: 10.1371/journal.pone.0020319.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell LineChromatin ImmunoprecipitationDNA PrimersElectrophoretic Mobility Shift AssayEpithelial CellsHumansLungOligonucleotide Array Sequence AnalysisPromoter Regions, GeneticProtein BindingReverse Transcriptase Polymerase Chain ReactionSmad3 ProteinTransforming Growth Factor beta1ConceptsGene expression microarraysLung epithelial cellsMolecular pathwaysTranscriptional regulationExpression microarraysGlobal transcriptional regulationTGFβ1/Smad3Epithelial cellsHuman promoter regionsSignal transduction cascadeTarget gene expressionEpithelial cell phenotypeGene expression analysisTranscription factor Smad3Primary lung epithelial cellsSmad3 targetsQuantitative real-time RT-PCRFOXA2 promoterHuman A549 alveolar epithelial cellsChromatin immunoprecipitationTransduction cascadeTarget genesA549 lung epithelial cellsExpression analysisGene expression
2006
Gene Expression Profiling as a Window into Idiopathic Pulmonary Fibrosis Pathogenesis
Kaminski N, Rosas IO. Gene Expression Profiling as a Window into Idiopathic Pulmonary Fibrosis Pathogenesis. Annals Of The American Thoracic Society 2006, 3: 339-344. PMID: 16738198, PMCID: PMC2658685, DOI: 10.1513/pats.200601-011tk.Peer-Reviewed Original ResearchConceptsIdiopathic pulmonary fibrosisModel of IPFIdiopathic pulmonary fibrosis (IPF) pathogenesisPulmonary fibrosis pathogenesisMinority of investigatorsPulmonary fibrosisFibrosis pathogenesisGene expression profilingMultiple diseasesExpression microarraysExpression profilingGene expressionMicroarrayIntegrative systems approach