2024
NADPH oxidase in B cells and macrophages protects against murine lupus by regulation of TLR7
Gordon R, Cosgrove H, Marinov A, Gingras S, Tilstra J, Campbell A, Bastacky S, Kashgarian M, Perl A, Nickerson K, Shlomchik M. NADPH oxidase in B cells and macrophages protects against murine lupus by regulation of TLR7. JCI Insight 2024, 9: e178563. PMID: 39042716, PMCID: PMC11343599, DOI: 10.1172/jci.insight.178563.Peer-Reviewed Original ResearchSystemic lupus erythematosusB cellsRegulation of TLR7Cell lineagesNADPH oxidaseHematopoietic cell lineagesNF-kB signalingSLE pathologyNOX2 deficiencyMyeloid compartmentMurine lupusLupus nephritisLupus erythematosusTLR7 signalingGlobal deletionInterstitial nephritisImmunomodulatory effectsGlobal knockoutConditional knockoutCYBBNOX2NephritisKnockoutTLR7Chimera approachIdentification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis
Moledina D, Obeid W, Smith R, Rosales I, Sise M, Moeckel G, Kashgarian M, Kuperman M, Campbell K, Lefferts S, Meliambro K, Bitzer M, Perazella M, Luciano R, Pober J, Cantley L, Colvin R, Wilson F, Parikh C. Identification and validation of urinary CXCL9 as a biomarker for diagnosis of acute interstitial nephritis. Journal Of Clinical Investigation 2024, 134: e180583. PMID: 38488004, PMCID: PMC10940080, DOI: 10.1172/jci180583.Peer-Reviewed Original Research
2022
Urine Uromodulin as a Biomarker of Kidney Tubulointerstitial Fibrosis
Melchinger H, Calderon-Gutierrez F, Obeid W, Xu L, Shaw MM, Luciano RL, Kuperman M, Moeckel GW, Kashgarian M, Wilson FP, Parikh CR, Moledina DG. Urine Uromodulin as a Biomarker of Kidney Tubulointerstitial Fibrosis. Clinical Journal Of The American Society Of Nephrology 2022, 17: 1284-1292. PMID: 35948365, PMCID: PMC9625093, DOI: 10.2215/cjn.04360422.Peer-Reviewed Original ResearchConceptsInterstitial fibrosis/tubular atrophyUrine uromodulinTubular atrophyThick ascending limbUrine albuminSerum creatinineKidney biopsyTubulointerstitial fibrosisMultivariable linear regression modelsTime of biopsyKidney's thick ascending limbAcademic medical centerHuman kidney biopsiesKidney tubulointerstitial fibrosisTubular healthMultivariable analysisHistologic featuresHistologic findingsHistologic changesKidney fibrosisIndependent associationFibrotic modelMultivariable modelMedical CenterMurine modelA Human MSH6 Germline Variant Associated With Systemic Lupus Erythematosus Induces Lupus‐like Disease in Mice
Meas R, Nititham J, Taylor KE, Maher S, Clairmont K, Carufe KEW, Kashgarian M, Nottoli T, Cheong A, Nagel ZD, Gaffney PM, Criswell LA, Sweasy JB. A Human MSH6 Germline Variant Associated With Systemic Lupus Erythematosus Induces Lupus‐like Disease in Mice. ACR Open Rheumatology 2022, 4: 760-770. PMID: 35708944, PMCID: PMC9469486, DOI: 10.1002/acr2.11471.Peer-Reviewed Original ResearchSystemic lupus erythematosusAntinuclear antibodiesMSH6 variantsLupus erythematosusMSH6 mutationsMouse modelSingle nucleotide polymorphismsDevelopment of SLELevel of ANAInfiltration of CD68Lupus-like diseaseInflammatory lung diseasesLung alveolar spacesRepair genesMismatch repair genesLung diseaseHealthy controlsPeyer's patchesWildtype miceAlveolar spaceCRISPR/Cas9 gene targetingSomatic hypermutation frequenciesAutoimmune phenotypeMiceDifferent mismatch repair genesPodocyte VEGF-A Knockdown Induces Diffuse Glomerulosclerosis in Diabetic and in eNOS Knockout Mice
Veron D, Aggarwal PK, Li Q, Moeckel G, Kashgarian M, Tufro A. Podocyte VEGF-A Knockdown Induces Diffuse Glomerulosclerosis in Diabetic and in eNOS Knockout Mice. Frontiers In Pharmacology 2022, 12: 788886. PMID: 35280251, PMCID: PMC8906751, DOI: 10.3389/fphar.2021.788886.Peer-Reviewed Original ResearchDiabetic kidney diseaseGlomerular filtration rateDiffuse glomerulosclerosisDiabetic miceRenal failureNitric oxideMassive proteinuriaKnockout miceEndothelial nitric oxide synthase knockout miceNitric oxide synthase knockout miceNormal glomerular filtration rateAbsence of diabetesENOS knockout miceSynthase knockout miceVascular endothelial growth factorTargetable molecular mechanismsFoot process effacementEndothelial growth factorAbnormal S-nitrosylationArteriolar hyalinosisGFR lossMild proteinuriaSevere proteinuriaKidney diseaseUrinary excretion
2021
Development and external validation of a diagnostic model for biopsy-proven acute interstitial nephritis using electronic health record data
Moledina DG, Eadon MT, Calderon F, Yamamoto Y, Shaw M, Perazella MA, Simonov M, Luciano R, Schwantes-An TH, Moeckel G, Kashgarian M, Kuperman M, Obeid W, Cantley LG, Parikh CR, Wilson FP. Development and external validation of a diagnostic model for biopsy-proven acute interstitial nephritis using electronic health record data. Nephrology Dialysis Transplantation 2021, 37: 2214-2222. PMID: 34865148, PMCID: PMC9755995, DOI: 10.1093/ndt/gfab346.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisInterstitial nephritisUrine biomarkersBiopsy-proven acute interstitial nephritisElectronic health record dataExternal validation cohortTypical clinical featuresBlood urea nitrogenTumor necrosis factorCharacteristic curve analysisHealth record dataExternal validationElectronic health recordsAIN diagnosisModest AUCsSerum creatinineCreatinine ratioKidney biopsyClinical featuresValidation cohortNecrosis factorUnrecognized casesInterleukin-9PatientsUrea nitrogenDNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model
Paluri SL, Burak M, Senejani AG, Levinson M, Rahim T, Clairmont K, Kashgarian M, Alvarado-Cruz I, Meas R, Cardó-Vila M, Zeiss C, Maher S, Bothwell ALM, Coskun E, Kant M, Jaruga P, Dizdaroglu M, Lloyd R, Sweasy JB. DNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model. DNA Repair 2021, 105: 103152. PMID: 34186496, PMCID: PMC8635285, DOI: 10.1016/j.dnarep.2021.103152.Peer-Reviewed Original ResearchIn memoriam: Robert Hodgson Heptinstall, 1920–2021
Kashgarian M, Racusen L. In memoriam: Robert Hodgson Heptinstall, 1920–2021. Kidney International 2021, 99: 782-783. DOI: 10.1016/j.kint.2021.01.014.Peer-Reviewed Original Research
2020
Urine interleukin-9 and tumor necrosis factor-α for prognosis of human acute interstitial nephritis
Moledina DG, Wilson FP, Kukova L, Obeid W, Luciano R, Kuperman M, Moeckel GW, Kashgarian M, Perazella MA, Cantley LG, Parikh CR. Urine interleukin-9 and tumor necrosis factor-α for prognosis of human acute interstitial nephritis. Nephrology Dialysis Transplantation 2020, 36: 1851-1858. PMID: 33125471, PMCID: PMC8476079, DOI: 10.1093/ndt/gfaa169.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisTumor necrosis factorCorticosteroid useIL-9Interstitial nephritisNecrosis factorAcute kidney injuryIL-9 levelsGlomerular filtration rateSubset of patientsCohort of participantsImmunosuppressive therapyTubulointerstitial infiltratesKidney injuryMedian eGFRKidney functionUrine biomarkersInterstitial fibrosisFiltration rateHistological featuresClinical trialsInterleukin-9PatientsEGFRComplete dataB cell-intrinsic TLR9 expression is protective in murine lupus
Tilstra JS, John S, Gordon RA, Leibler C, Kashgarian M, Bastacky S, Nickerson KM, Shlomchik MJ. B cell-intrinsic TLR9 expression is protective in murine lupus. Journal Of Clinical Investigation 2020, 130: 3172-3187. PMID: 32191633, PMCID: PMC7260024, DOI: 10.1172/jci132328.Peer-Reviewed Original ResearchConceptsToll-like receptor 9Systemic lupus erythematosusTLR9 deficiencyTLR9 expressionDendritic cellsB cellsPlasmacytoid dendritic cellsAnti-nucleosome antibodiesLupus erythematosusAutoantibody productionMurine lupusSLE pathogenesisTLR9 signalsReceptor 9Cell type-specific effectsType-specific effectsDisease pathogenesisTherapeutic potentialSelective deletionDiseaseNonredundant roleNephritisLupusPathogenesisDeficiency
2019
Urine TNF-α and IL-9 for clinical diagnosis of acute interstitial nephritis
Moledina DG, Wilson FP, Pober JS, Perazella MA, Singh N, Luciano RL, Obeid W, Lin H, Kuperman M, Moeckel GW, Kashgarian M, Cantley LG, Parikh CR. Urine TNF-α and IL-9 for clinical diagnosis of acute interstitial nephritis. JCI Insight 2019, 4: e127456. PMID: 31092735, PMCID: PMC6542610, DOI: 10.1172/jci.insight.127456.Peer-Reviewed Original ResearchConceptsAcute interstitial nephritisAcute kidney diseasePrebiopsy diagnosisKidney biopsyKidney diseaseIL-9AIN diagnosisUrine TNFInterstitial nephritisSpecific T cell subsetsAcute tubular injuryDiabetic kidney diseaseIL-9 levelsTNF-α levelsT cell subsetsAddition of biomarkersPlasma cytokinesCytokine levelsTubular injuryHighest quartileMultivariable analysisCell subsetsUrinary TNFBlood eosinophilsGlomerular disease
2017
Chapter 10 Renal Neoplasia
Fogo A, Kashgarian M. Chapter 10 Renal Neoplasia. 2017, 511-530. DOI: 10.1016/b978-0-323-39053-8.00010-1.Peer-Reviewed Original ResearchPreface
Fogo A, Kashgarian M. Preface. 2017, v. DOI: 10.1016/b978-0-323-39053-8.05001-2.Peer-Reviewed Original ResearchChapter 3 Glomerular Diseases
Fogo A, Kashgarian M. Chapter 3 Glomerular Diseases. 2017, 19-294.e1. DOI: 10.1016/b978-0-323-39053-8.00003-4.Peer-Reviewed Original ResearchChapter 1 Approach to Diagnosis of the Kidney Biopsy
Fogo A, Kashgarian M. Chapter 1 Approach to Diagnosis of the Kidney Biopsy. 2017, 1-7. DOI: 10.1016/b978-0-323-39053-8.00001-0.Peer-Reviewed Original ResearchChapter 2 Normal Growth and Maturation
Fogo A, Kashgarian M. Chapter 2 Normal Growth and Maturation. 2017, 9-17. DOI: 10.1016/b978-0-323-39053-8.00002-2.Peer-Reviewed Original ResearchChapter 9 Cystic Diseases of the Kidney
Fogo A, Kashgarian M. Chapter 9 Cystic Diseases of the Kidney. 2017, 491-509. DOI: 10.1016/b978-0-323-39053-8.00009-5.Peer-Reviewed Original ResearchChapter 4 Vascular Diseases
Fogo A, Kashgarian M. Chapter 4 Vascular Diseases. 2017, 295-364. DOI: 10.1016/b978-0-323-39053-8.00004-6.Peer-Reviewed Original ResearchChapter 8 Renal Transplantation
Fogo A, Kashgarian M. Chapter 8 Renal Transplantation. 2017, 463-489. DOI: 10.1016/b978-0-323-39053-8.00008-3.Peer-Reviewed Original ResearchChapter 5 Tubulointerstitial Diseases
Fogo A, Kashgarian M. Chapter 5 Tubulointerstitial Diseases. 2017, 365-448. DOI: 10.1016/b978-0-323-39053-8.00005-8.Peer-Reviewed Original Research