2024
Multimodal measures of spontaneous brain activity reveal both common and divergent patterns of cortical functional organization
Vafaii H, Mandino F, Desrosiers-Grégoire G, O’Connor D, Markicevic M, Shen X, Ge X, Herman P, Hyder F, Papademetris X, Chakravarty M, Crair M, Constable R, Lake E, Pessoa L. Multimodal measures of spontaneous brain activity reveal both common and divergent patterns of cortical functional organization. Nature Communications 2024, 15: 229. PMID: 38172111, PMCID: PMC10764905, DOI: 10.1038/s41467-023-44363-z.Peer-Reviewed Original Research
2023
Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior
Benisty H, Barson D, Moberly A, Lohani S, Tang L, Coifman R, Crair M, Mishne G, Cardin J, Higley M. Rapid fluctuations in functional connectivity of cortical networks encode spontaneous behavior. Nature Neuroscience 2023, 27: 148-158. PMID: 38036743, PMCID: PMC11316935, DOI: 10.1038/s41593-023-01498-y.Peer-Reviewed Original ResearchConceptsFunctional connectivitySpontaneous behaviorCortical networksCortical network activityTime-varying functional connectivityFunctional magnetic resonanceCerebral cortexAwake miceDynamic functional connectivityAwake animalsNeighboring neuronsPatterned activityDistinct behavioral statesTwo-photon microscopyNeural activityCortical signalsBehavioral statesCortexNetwork activityCortical dynamicsMagnetic resonance
2022
Functional network properties derived from wide-field calcium imaging differ with wakefulness and across cell type
O’Connor D, Mandino F, Shen X, Horien C, Ge X, Herman P, Hyder F, Crair M, Papademetris X, Lake E, Constable. Functional network properties derived from wide-field calcium imaging differ with wakefulness and across cell type. NeuroImage 2022, 264: 119735. PMID: 36347441, PMCID: PMC9808917, DOI: 10.1016/j.neuroimage.2022.119735.Peer-Reviewed Original Research
2020
Retinal and Callosal Activity-Dependent Chandelier Cell Elimination Shapes Binocularity in Primary Visual Cortex
Wang BS, Bernardez Sarria MS, An X, He M, Alam NM, Prusky GT, Crair MC, Huang ZJ. Retinal and Callosal Activity-Dependent Chandelier Cell Elimination Shapes Binocularity in Primary Visual Cortex. Neuron 2020, 109: 502-515.e7. PMID: 33290732, PMCID: PMC7943176, DOI: 10.1016/j.neuron.2020.11.004.Peer-Reviewed Original ResearchConceptsPrimary visual cortexVisual cortexTranscallosal pathwayVisual fieldDeficient binocular visionGABAergic chandelier cellsBinocular circuitsBinocular visionChandelier cellsRetinal activityTranscallosal projectionsGeniculocortical inputCallosal activityCenter visual fieldBinocular regionCortexMassive apoptosisDevelopmental assemblyCritical periodV1IpsiBlockadePathwayBinocularityMiceMesoscopic Imaging: Shining a Wide Light on Large-Scale Neural Dynamics
Cardin JA, Crair MC, Higley MJ. Mesoscopic Imaging: Shining a Wide Light on Large-Scale Neural Dynamics. Neuron 2020, 108: 33-43. PMID: 33058764, PMCID: PMC7577373, DOI: 10.1016/j.neuron.2020.09.031.Peer-Reviewed Original Research
2019
Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits
Barson D, Hamodi AS, Shen X, Lur G, Constable RT, Cardin JA, Crair MC, Higley MJ. Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. Nature Methods 2019, 17: 107-113. PMID: 31686040, PMCID: PMC6946863, DOI: 10.1038/s41592-019-0625-2.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptide-expressing interneuronsBrain-wide network dynamicsTwo-photon calcium imagingEntire cortical mantleLocal neuronal circuitsLong-range cortical networksWidespread gene deliveryWidefield calciumPyramidal neuronsAwake miceCortical circuitsNeuronal activityCortical mantleTwo-photon imagingViral transduction methodsNeuronal circuitsCalcium imagingMouse brainFunctional connectivityLocal microcircuitsMammalian cortexCortical architectureCortical networksBrainActivity propagates
2017
Architecture, Function, and Assembly of the Mouse Visual System
Seabrook TA, Burbridge TJ, Crair MC, Huberman AD. Architecture, Function, and Assembly of the Mouse Visual System. Annual Review Of Neuroscience 2017, 40: 499-538. PMID: 28772103, DOI: 10.1146/annurev-neuro-071714-033842.Peer-Reviewed Original ResearchConceptsSpecific cell typesMammalian central nervous systemGenetic perturbationsModel speciesCell typesCausal testingSpeciesVivo labelingBroad scaleVisual system structuresManipulation of neuronsSensory systemsCentral nervous systemVisual circuitryPowerful toolNervous systemLarge cadreHumansMouse visual systemAdditional experimentationPathwayFunctionConvenient sizeAssemblyVisual system
2013
Laminar and Columnar Development of Barrel Cortex Relies on Thalamocortical Neurotransmission
Li H, Fertuzinhos S, Mohns E, Hnasko TS, Verhage M, Edwards R, Sestan N, Crair MC. Laminar and Columnar Development of Barrel Cortex Relies on Thalamocortical Neurotransmission. Neuron 2013, 79: 970-986. PMID: 24012009, PMCID: PMC3768017, DOI: 10.1016/j.neuron.2013.06.043.Peer-Reviewed Original ResearchConceptsThalamocortical neurotransmissionCortical developmentNeuronal differentiationGlutamatergic synaptic transmissionSuperficial cortical laminaeEarly cortical developmentActivity-dependent factorsThalamocortical neuronsSomatosensory cortexCortical cytoarchitectureCortical laminationSynaptic transmissionNeuronal activityCortical laminaeCytoarchitectonic criteriaExtrinsic activityNeurotransmissionCortical columnsMolecular cuesNeuronsMorphologic developmentLittle evidenceColumnar developmentGene expressionExtrinsic factorsCompetition driven by retinal waves promotes morphological and functional synaptic development of neurons in the superior colliculus
Furman M, Xu HP, Crair MC. Competition driven by retinal waves promotes morphological and functional synaptic development of neurons in the superior colliculus. Journal Of Neurophysiology 2013, 110: 1441-1454. PMID: 23741047, PMCID: PMC3763158, DOI: 10.1152/jn.01066.2012.Peer-Reviewed Original ResearchConceptsSuperior colliculusRetinal wavesRetinal inputBrain slice preparationActivity-dependent competitionWT miceRetinofugal axonsSlice preparationSC neuronsTransgenic miceBrain regionsSynaptic strengthSynaptic developmentSynapse developmentMiceNeuronsEye openingFunctional developmentSynapsesColliculusMolecular mechanismsSpecific roleInstructive roleMorphological developmentAxons
2011
An Instructive Role for Patterned Spontaneous Retinal Activity in Mouse Visual Map Development
Xu HP, Furman M, Mineur YS, Chen H, King SL, Zenisek D, Zhou ZJ, Butts DA, Tian N, Picciotto MR, Crair MC. An Instructive Role for Patterned Spontaneous Retinal Activity in Mouse Visual Map Development. Neuron 2011, 70: 1115-1127. PMID: 21689598, PMCID: PMC3119851, DOI: 10.1016/j.neuron.2011.04.028.Peer-Reviewed Original ResearchConceptsSpontaneous retinal activityRetinal activityRetinal ganglion cell projectionsEye-specific segregationGanglion cell projectionsSpontaneous retinal wavesActivity-dependent refinementRetinal ganglion cellsMouse visual systemComplex neural circuitsEye of originRetinal wavesGanglion cellsRetinotopic refinementNeuronal activitySpontaneous activityMammalian visual systemAcetylcholine receptorsNeuronal connectivityMammalian brainNeural circuitsOverall activity levelsActivity levelsBrainVisual system
2007
Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice
Albright MJ, Weston MC, Inan M, Rosenmund C, Crair MC. Increased Thalamocortical Synaptic Response and Decreased Layer IV Innervation in GAP-43 Knockout Mice. Journal Of Neurophysiology 2007, 98: 1610-1625. PMID: 17581849, DOI: 10.1152/jn.00219.2007.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic potentialsField excitatory postsynaptic potentialsGAP-43Thalamocortical synapsesSynaptic responsesCompetitive glutamate receptor antagonistN-methyl-D-aspartate receptorsAcute brain slice preparationBarrel map formationThalamocortical synaptic responsesWild-type littermate controlsGlutamate receptor antagonistsBrain slice preparationGrowth-associated proteinThalamic innervationThalamic neuronsBarrel mapReceptor antagonistIsoxazolepropionate (AMPA) receptorsPostsynaptic potentialsLayer IVSlice preparationBarrel cortexSynaptic transmissionAMPAR functionDevelopmental Homeostasis of Mouse Retinocollicular Synapses
Chandrasekaran AR, Shah RD, Crair MC. Developmental Homeostasis of Mouse Retinocollicular Synapses. Journal Of Neuroscience 2007, 27: 1746-1755. PMID: 17301182, PMCID: PMC6673732, DOI: 10.1523/jneurosci.4383-06.2007.Peer-Reviewed Original ResearchMeSH KeywordsAlpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic AcidAnimalsAnimals, NewbornBrain MappingExcitatory Amino Acid AgonistsHomeostasisMembrane PotentialsMiceMice, Inbred C57BLMice, KnockoutModels, BiologicalNeuronsN-MethylaspartateReceptors, NicotinicRetinaSuperior ColliculiSynapsesVisual CortexVisual PathwaysConceptsRetinal wavesBeta2-/- miceSpontaneous retinal wavesRetinal ganglion cellsWild-type miceActivity-dependent competitionFirst postnatal weekTotal integrated responseLarge retinal areasTotal synaptic inputNeuronal receptive fieldsReceptive fieldsGanglion cellsPerturbation of activitiesSynaptic transmissionPostnatal weekResponse homeostasisSynaptic inputsRetinal areaRetinal inputSuperior colliculusStrong synapsesVisual cortexMutant miceRetinotopic mapping
2005
Mutations in Drosophila sec15 Reveal a Function in Neuronal Targeting for a Subset of Exocyst Components
Mehta SQ, Hiesinger PR, Beronja S, Zhai RG, Schulze KL, Verstreken P, Cao Y, Zhou Y, Tepass U, Crair MC, Bellen HJ. Mutations in Drosophila sec15 Reveal a Function in Neuronal Targeting for a Subset of Exocyst Components. Neuron 2005, 46: 219-232. PMID: 15848801, DOI: 10.1016/j.neuron.2005.02.029.Peer-Reviewed Original ResearchConceptsExocyst componentsComplex of proteinsCell polaritySec15Specific cell adhesionSpecific proteinsMutant neuronsSubcellular distributionCell adhesionCell lethalityExocystNeuronal targetingSec5Neurite outgrowthSynaptic specificityCell viabilityProteinSeparable functionsSec6Sec8SubcomplexMislocalizationYeastGenesComponents persistDistinct developmental programs require different levels of Bmp signaling during mouse retinal development
Murali D, Yoshikawa S, Corrigan RR, Plas DJ, Crair MC, Oliver G, Lyons KM, Mishina Y, Furuta Y. Distinct developmental programs require different levels of Bmp signaling during mouse retinal development. Development 2005, 132: 913-923. PMID: 15673568, DOI: 10.1242/dev.01673.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBody PatterningBone Morphogenetic Protein Receptors, Type IBone Morphogenetic ProteinsCell ProliferationChromosome MappingFibroblast Growth FactorsGene Expression Regulation, DevelopmentalImmunohistochemistryIn Situ HybridizationMiceMice, TransgenicModels, BiologicalModels, GeneticMutationNeuronsOptic NerveProtein Serine-Threonine KinasesReceptors, Growth FactorRetinaSignal TransductionTransgenesConceptsDistinct developmental programsDevelopmental programRetinal developmentBMP receptor activityReceptor geneCell-autonomous requirementCre-loxP strategySevere eye defectsDirect genetic evidenceEmbryonic tissue interactionsMouse retinal developmentEye abnormalitiesMutant backgroundDorsoventral patterningMouse retinaReceptor activityDouble mutantReceptor functionEmbryonic retinaBMP familyRedundant rolesRetinal neurogenesisFunctional copyEmbryonic developmentGenetic evidence
2000
Emergence of ocular dominance columns in cat visual cortex by 2 weeks of age
Crair M, Horton J, Antonini A, Stryker M. Emergence of ocular dominance columns in cat visual cortex by 2 weeks of age. The Journal Of Comparative Neurology 2000, 430: 235-249. PMID: 11135259, PMCID: PMC2412906, DOI: 10.1002/1096-9861(20010205)430:2<235::aid-cne1028>3.0.co;2-p.Peer-Reviewed Original ResearchConceptsOcular dominance columnsCat visual cortexOcular dominance column formationWeeks of ageGeniculocortical projectionsGeniculocortical afferentsVisual cortexGeniculocortical afferent segregationPostnatal day 14Lateral geniculate nucleus inputsArea of cortexPrevious anatomic studiesRetrograde labelingOcular dominance patternsAnatomic correlatesAnatomic studyVisual deprivationTransneuronal labelAfferent segregationDay 14Eye dominanceAfferentsAnatomic dataCortexSecond week
1999
The Nuclear Orphan Receptor COUP-TFI Is Required for Differentiation of Subplate Neurons and Guidance of Thalamocortical Axons
Zhou C, Qiu Y, Pereira F, Crair M, Tsai S, Tsai M. The Nuclear Orphan Receptor COUP-TFI Is Required for Differentiation of Subplate Neurons and Guidance of Thalamocortical Axons. Neuron 1999, 24: 847-859. PMID: 10624948, DOI: 10.1016/s0896-6273(00)81032-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimetabolitesAxonsBromodeoxyuridineCarbocyaninesCell DeathCell DifferentiationCerebral CortexCOUP Transcription Factor IDNA-Binding ProteinsFluorescent DyesImmunohistochemistryIn Situ HybridizationMaleMiceMutationNeural PathwaysNeuronsReceptors, GlucocorticoidThalamusTranscription FactorsConceptsSubplate neuronsThalamocortical projectionsCortical layer IVLayer IV neuronsCell deathCorticothalamic connectivityAfferent innervationCerebral cortexThalamocortical axonsLayer IVNervous systemExcessive cell deathFactor INeuronal developmentNeuronsNuclear receptorsPremature cell deathInnervationImproper differentiationImportant regulatorOrphan memberDeathCritical roleDifferentiationFailureAltered spatial patterns of functional thalamocortical connections in the barrel cortex after neonatal infraorbital nerve cut revealed by optical recording
Higashi S, Crair MC, Kurotani T, Inokawa H, Toyama K. Altered spatial patterns of functional thalamocortical connections in the barrel cortex after neonatal infraorbital nerve cut revealed by optical recording. Neuroscience 1999, 91: 439-452. PMID: 10366001, DOI: 10.1016/s0306-4522(98)00666-6.Peer-Reviewed Original ResearchConceptsInfraorbital nerve cutNerve cutNormal ratsLayer IVSomatosensory cortexDextran amine labelingThalamocortical slice preparationPostnatal day 7Cytochrome oxidase stainingThalamocortical transmissionThalamocortical connectionsDextran amineThalamocortical axonsThalamic stimulationBarrel cortexFunctional synapsesSlice preparationAxon terminalsVoltage-sensitive dyeTerminal arborsAltered spatial patternDay 7P5-P6RatsBarrel formation