Featured Publications
HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease
Little A, Chen P, Vesely M, Khan R, Fiedler J, Garritano J, Islam F, McNiff J, Craft J. HIF-1 regulates pathogenic cytotoxic T cells in lupus skin disease. JCI Insight 2023, 8: e166076. PMID: 37526979, PMCID: PMC10543720, DOI: 10.1172/jci.insight.166076.Peer-Reviewed Original ResearchConceptsCutaneous lupus erythematosusLupus skin diseaseT cellsSkin diseasesCytotoxic signatureInflammatory infiltrateHIF-1Tissue damageKidney-infiltrating T cellsSkin-infiltrating T cellsAutoimmune skin diseaseHIF-1 inhibitionSkin tissue damageLupus erythematosusSystemic diseaseTissue inflammationGranzyme BMouse modelInflammatory gene programDiseaseProtein levelsInfiltratesSkin environmentGene programPresent studySpatial Transcriptomics in Inflammatory Skin Diseases Using GeoMx Digital Spatial Profiling: A Practical Guide for Applications in Dermatology
Cho C, Haddadi N, Kidacki M, Woodard G, Shakiba S, Yıldız-Altay Ü, Richmond J, Vesely M. Spatial Transcriptomics in Inflammatory Skin Diseases Using GeoMx Digital Spatial Profiling: A Practical Guide for Applications in Dermatology. JID Innovations 2024, 5: 100317. PMID: 39559817, PMCID: PMC11570843, DOI: 10.1016/j.xjidi.2024.100317.Peer-Reviewed Original ResearchDigital spatial profilingInflammatory skin diseaseGeoMx Digital Spatial ProfilerSkin diseasesDiscoid lupus erythematosusNanoString GeoMx Digital Spatial ProfilingImmune milieuImmune microenvironmentLichen planusLupus erythematosusSingle cell RNA sequencingAberrant inflammationCell RNA sequencingSkin disease pathogenesisDisease pathogenesisManufacturer's guidelinesIndicators of diseaseDiseaseSpatial transcriptomics platformSkinTissue processing
2021
Cytokine RNA In Situ Hybridization Permits Individualized Molecular Phenotyping in Biopsies of Psoriasis and Atopic Dermatitis
Wang A, Fogel AL, Murphy MJ, Panse G, McGeary MK, McNiff JM, Bosenberg M, Vesely MD, Cohen JM, Ko CJ, King BA, Damsky W. Cytokine RNA In Situ Hybridization Permits Individualized Molecular Phenotyping in Biopsies of Psoriasis and Atopic Dermatitis. JID Innovations 2021, 1: 100021. PMID: 34909719, PMCID: PMC8659380, DOI: 10.1016/j.xjidi.2021.100021.Peer-Reviewed Original ResearchAtopic dermatitisAtopic dermatitis casesInflammatory skin diseaseInflammatory skin disorderSubset of casesSitu hybridizationCytokine RNARoutine biopsyCytokine expressionPredominant cytokinePsoriasis casesCytokine targetsTreatment selectionDermatitis casesSkin disordersPsoriasis biomarkersSkin diseasesIndividual cytokinesIndividualized evaluationPsoriasisBiopsyBulk RNA sequencingCytokinesMolecular phenotypingPatients