Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function
Firnhaber C, Hammarlund M. Neuron-Specific Feeding RNAi in C. elegans and Its Use in a Screen for Essential Genes Required for GABA Neuron Function. PLOS Genetics 2013, 9: e1003921. PMID: 24244189, PMCID: PMC3820814, DOI: 10.1371/journal.pgen.1003921.Peer-Reviewed Original ResearchConceptsCell-specific knockdownEssential genesFeeding RNAiC. elegansGenetic requirementsNeuronal functionForward genetic screenGABA neuron functionGene function resultsForward screenGenetic screenRNAi resultsRNAi strainsChromosome IRNAi clonesGlutamate-releasing neuronsGene actionGene knockdownMature organismRNAiGenesKnockdownNeuron functionElegansPleiotropyRapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed
Williams DC, Bejjani RE, Ramirez PM, Coakley S, Kim SA, Lee H, Wen Q, Samuel A, Lu H, Hilliard MA, Hammarlund M. Rapid and Permanent Neuronal Inactivation In Vivo via Subcellular Generation of Reactive Oxygen with the Use of KillerRed. Cell Reports 2013, 5: 553-563. PMID: 24209746, PMCID: PMC3877846, DOI: 10.1016/j.celrep.2013.09.023.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesC. elegansGenetic toolsOrganelle fragmentationPlasma membraneSpecific developmental outcomesSubcellular responsesCell deathKillerRedReactive oxygenOxygen speciesTargeted cellCircuit functionSingle light stimulusSingle animalInactivationElegansCellsVivoCell bodiesSpeciesNeuronal degenerationNeuronsAnimalsNeuronal inactivation