2019
Measurement of Chitinase Activity in Biological Samples.
Amick AK, Liu Q, Gautam S, Chupp G, Dela Cruz CS, Sharma L. Measurement of Chitinase Activity in Biological Samples. Journal Of Visualized Experiments 2019 PMID: 31498326, DOI: 10.3791/60159.Peer-Reviewed Original ResearchConceptsDisease severityType 2 inflammatory diseasesPulmonary fibrosisAllergic diseasesInflammatory diseasesHost immunityReliable biomarkersTherapeutic effectivenessGaucher diseaseHost defenseSerum samplesDiseaseChitinase-like proteinsGenetic disordersHuman subjectsPrecise roleImportant biomarkerBiomarkersSeverityImportant roleChitinase levelsAsthmaPatientsFibrosisMice
2018
The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size
Fuchs K, Cardona Gloria Y, Wolz O, Herster F, Sharma L, Dillen CA, Täumer C, Dickhöfer S, Bittner Z, Dang T, Singh A, Haischer D, Schlöffel MA, Koymans KJ, Sanmuganantham T, Krach M, Roger T, Le Roy D, Schilling NA, Frauhammer F, Miller LS, Nürnberger T, LeibundGut‐Landmann S, Gust AA, Macek B, Frank M, Gouttefangeas C, Dela Cruz CS, Hartl D, Weber AN. The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size. EMBO Reports 2018, 19: embr201846065. PMID: 30337494, PMCID: PMC6280652, DOI: 10.15252/embr.201846065.Peer-Reviewed Original ResearchConceptsTLR2 ligandsInnate immune receptors Toll-like receptorToll-like receptorsMurine immune cellsImmune activationDistinct signaling outcomesImmune cellsNovel therapiesTriggers inflammationImmuno-modulationFungal infectionsInflammationImmune receptorsPotential targetTLR2Unknown purityReceptorsNanomolar affinityAsthmaHumansTherapyInfectionFungal diseasesDiseasePathology
2017
Chitin and Its Effects on Inflammatory and Immune Responses
Elieh Ali Komi D, Sharma L, Dela Cruz CS. Chitin and Its Effects on Inflammatory and Immune Responses. Clinical Reviews In Allergy & Immunology 2017, 54: 213-223. PMID: 28251581, PMCID: PMC5680136, DOI: 10.1007/s12016-017-8600-0.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsImmune cellsCytokine productionImmune systemAdaptive immune cellsAlternative macrophage activationHost immune systemPathogen-associated molecular patternsPattern recognition receptorsTLR-2Mammalian immune systemImmunological aspectsAllergic responsesIntraperitoneal administrationLeukocyte recruitmentImmune responseAdaptive immunityMacrophage activationImmune recognitionRecognition receptorsTrue chitinasesNKR-P1Molecular patternsPotential targetMammalian chitinasesMammalian receptors