2020
Differential effects of the Akt pathway on the internalization of Klebsiella by lung epithelium and macrophages
Chang D, Feng J, Liu H, Liu W, Sharma L, Dela Cruz CS. Differential effects of the Akt pathway on the internalization of Klebsiella by lung epithelium and macrophages. Innate Immunity 2020, 26: 618-626. PMID: 32762278, PMCID: PMC7556185, DOI: 10.1177/1753425920942582.Peer-Reviewed Original ResearchConceptsAkt pathwayEpithelial cellsCell typesKey cellular pathwaysLung epitheliumActivation of AktPhagocytic abilityMultiple cell typesAkt inhibitor MK2206Particular bacterial infectionsCellular pathwaysLung epithelial cellsMacrophage cell lineHost cellsBacterial infectionsRespiratory epithelial cellsCell proliferationType of infectionCell linesPathwayHost defenseDifferential rolesAktSC-79CellsMechanisms of Epithelial Immunity Evasion by Respiratory Bacterial Pathogens
Sharma L, Feng J, Britto CJ, Dela Cruz CS. Mechanisms of Epithelial Immunity Evasion by Respiratory Bacterial Pathogens. Frontiers In Immunology 2020, 11: 91. PMID: 32117248, PMCID: PMC7027138, DOI: 10.3389/fimmu.2020.00091.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsBacterial lung infectionsImmune cellsBacterial clearanceRespiratory bacterial pathogensEpithelial cellsLung infectionSecretion of cytokinesEpithelial host defenseMuco-ciliary clearanceHuge economic burdenRespiratory epithelial cellsLung epithelial surfaceMajor healthcare challengeEpithelial immune mechanismsBacterial pathogensAntimicrobial peptide productionImmune mechanismsImmune protectionMucus productionEconomic burdenPathogen clearanceEpithelial immunityHost defenseClinical researchEpithelial resistance
2019
Measurement of Chitinase Activity in Biological Samples.
Amick AK, Liu Q, Gautam S, Chupp G, Dela Cruz CS, Sharma L. Measurement of Chitinase Activity in Biological Samples. Journal Of Visualized Experiments 2019 PMID: 31498326, DOI: 10.3791/60159.Peer-Reviewed Original ResearchConceptsDisease severityType 2 inflammatory diseasesPulmonary fibrosisAllergic diseasesInflammatory diseasesHost immunityReliable biomarkersTherapeutic effectivenessGaucher diseaseHost defenseSerum samplesDiseaseChitinase-like proteinsGenetic disordersHuman subjectsPrecise roleImportant biomarkerBiomarkersSeverityImportant roleChitinase levelsAsthmaPatientsFibrosisMice
2018
Regional Differences in Airway Epithelial Cells Reveal Tradeoff between Defense against Oxidative Stress and Defense against Rhinovirus
Mihaylova VT, Kong Y, Fedorova O, Sharma L, Dela Cruz CS, Pyle AM, Iwasaki A, Foxman EF. Regional Differences in Airway Epithelial Cells Reveal Tradeoff between Defense against Oxidative Stress and Defense against Rhinovirus. Cell Reports 2018, 24: 3000-3007.e3. PMID: 30208323, PMCID: PMC6190718, DOI: 10.1016/j.celrep.2018.08.033.Peer-Reviewed Original ResearchConceptsRIG-I stimulationAntiviral responseRhinovirus infectionBronchial airway epithelial cellsAcute respiratory infectionsEpithelial cellsRobust antiviral responseAirway epithelial cellsPrimary human nasalAirway damageRespiratory infectionsAirway microenvironmentAsthma attacksNasal mucosaLeading causeNrf2 knockdownNasal cellsNrf2 activationHuman nasalEpithelial defenseHost defenseBronchial cellsInfectionOxidative stressRhinovirus