2015
TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE
Zhao PY, Gan G, Peng S, Wang SB, Chen B, Adelman RA, Rizzolo LJ. TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE. Investigative Ophthalmology & Visual Science 2015, 56: 1916-1923. PMID: 25736794, PMCID: PMC4364639, DOI: 10.1167/iovs.14-15738.Peer-Reviewed Original ResearchConceptsHuman fetal RPETRP channelsApical membraneFetal RPEApical plasma membraneCell-cell contactTransepithelial electrical resistanceTransient receptor potential channelsTight junctionsSubcellular localizationInhibitor of calpainPrimary ciliaRT-PCRPlasma membraneBasal channel activityQuantitative RT-PCRApical tight junctionsExpression of TRPC4Ion channelsBasolateral surfaceApical microvilliApical surfaceChannel activityConfocal microscopyTRPM3
1998
Polarization of the Na+, K+-ATPase in Epithelia Derived from the Neuroepithelium
Rizzolo L. Polarization of the Na+, K+-ATPase in Epithelia Derived from the Neuroepithelium. International Review Of Cytology 1998, 185: 195-235. PMID: 9750268, DOI: 10.1016/s0074-7696(08)60152-7.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsProper membrane domainDifferent sorting signalsPolarity mechanismsSorting signalsMembrane domainsMembrane proteinsCortical cytoskeletonCertain environmental stimuliFascinating groupMaintenance signalsApical membraneEnvironmental stimuliIon pumpsProteinBasolateral membraneATPaseYields insightsNeuroepitheliumMembraneCellsCytoskeletonEpitheliumRetinal pigment epitheliumSignalsPigment epithelium
1995
The distribution of Na+,K+-atpase and 5a11 antigen in apical microvilli of the retinal pigment epithelium is unrelated to α-spectrin
Rizzolo L, Zhou S. The distribution of Na+,K+-atpase and 5a11 antigen in apical microvilli of the retinal pigment epithelium is unrelated to α-spectrin. Journal Of Cell Science 1995, 108: 3623-3633. PMID: 8586673, DOI: 10.1242/jcs.108.11.3623.Peer-Reviewed Original ResearchConceptsAlpha-spectrinPlasma membranePlasma membrane proteinsDevelopment of polarityLateral plasma membraneBasal plasma membraneMembrane proteinsCortical cytoskeletonMacromolecular complexesBeta spectrinAnkyrinChick embryogenesisApical poleCytoskeletonEmbryonic day 6RPE microvilliLateral membranesReceptor proteinMost epitheliaSpectrin distributionΑ-spectrinApical membraneIon pumpsApical microvilliProtein
1994
The neural retina maintains integrins in the apical membrane of the RPE early in development.
Rizzolo L, Zhou S, Li Z. The neural retina maintains integrins in the apical membrane of the RPE early in development. Investigative Ophthalmology & Visual Science 1994, 35: 2567-76. PMID: 8163344.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumNeural retinaAlpha 6Alpha 3Photoreceptor cell layerSubunit alpha 3Apical membraneBasolateral membraneBeta 1 subunitPigment epitheliumDistribution of integrinsBeta 1 familyRetinaBeta 1Interphotoreceptor matrixExplant culturesCell layerBasal membraneRetinal interfaceEpitheliumIndividual integrinsIntegrin distributionIntegrinsIntercellular interactionsChicken development
1993
Apical Orientation of the Microtubule Organizing Center and Associated γ-Tubulin during the Polarization of the Retinal Pigment Epithelium in Vivo
Rizzolo L, Joshi H. Apical Orientation of the Microtubule Organizing Center and Associated γ-Tubulin during the Polarization of the Retinal Pigment Epithelium in Vivo. Developmental Biology 1993, 157: 147-156. PMID: 8482407, DOI: 10.1006/dbio.1993.1119.Peer-Reviewed Original ResearchConceptsMicrotubule arraysCell polarityEmbryonic day 3Apical membraneGamma-tubulin fociJunctional complexesSimple epithelial cellsMicrotubule organizing centerDevelopment of epitheliaCentrosomal proteinsJuxtanuclear regionΓ-tubulinUnique reorganizationBasal axisGamma-tubulinCell-basement membrane interactionsFunctional polarityMicrotubule growthMembrane interactionsOrganizing centerPrimary cell culturesCultured cellsEpithelial cellsRetinal pigment epitheliumCell cultures
1990
The distribution of Na+,K+-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture
Rizzolo L. The distribution of Na+,K+-ATPase in the retinal pigmented epithelium from chicken embryo is polarized in vivo but not in primary cell culture. Experimental Eye Research 1990, 51: 435-446. PMID: 2170160, DOI: 10.1016/0014-4835(90)90156-o.Peer-Reviewed Original ResearchConceptsBasolateral plasma membranePlasma membranePrimary cell culturesPlasma membrane proteinsFunctional tight junctionsCultured RPEChicken embryosMatrix-coated filtersExtracellular matrix coatingCell culturesMembrane proteinsVesicular stomatitis virusMorphological polarityApical poleCultured cellsApical membraneEngelbreth-HolmJunctional complexesSwarm tumorEmbryonic eyeStomatitis virusATPaseBasolateral membraneSerum-free mediumTight junctions