1977
Kinetic mechanism of tRNA nucleotidyltransferase from Escherichia coli.
Williams K, Schofield P. Kinetic mechanism of tRNA nucleotidyltransferase from Escherichia coli. Journal Of Biological Chemistry 1977, 252: 5589-5597. PMID: 18468, DOI: 10.1016/s0021-9258(19)63391-0.Peer-Reviewed Original ResearchPurification and some properties of Escherichia coli tRNA nucleotidyltransferase.
Schofield P, Williams K. Purification and some properties of Escherichia coli tRNA nucleotidyltransferase. Journal Of Biological Chemistry 1977, 252: 5584-5588. PMID: 328503, DOI: 10.1016/s0021-9258(19)63390-9.Peer-Reviewed Original ResearchConceptsTransition metal chelating agentsMetal chelating agentsSodium dodecyl sulfate gel electrophoresisDodecyl sulfate gel electrophoresisSulfate gel electrophoresisTurnover numberChelating agentOverall yieldMolecular weightPure enzymeIsoelectric pointKey stepIdentical isoelectric pointsSephadex chromatographyCrude extractPurificationAffinity columnGel electrophoresisEscherichia coli tRNA nucleotidyltransferaseSpecific activityAssay conditionsChromatographyEnzymeTRNA nucleotidyltransferaseOptimal assay conditions
1975
Evidence for metalloenzyme character of tRNA nucleotidyl transferase
Williams K, Schofield P. Evidence for metalloenzyme character of tRNA nucleotidyl transferase. Biochemical And Biophysical Research Communications 1975, 64: 262-267. PMID: 238508, DOI: 10.1016/0006-291x(75)90247-8.Peer-Reviewed Original Research