1996
Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA‐3 signaling pathway
Karmann K, Hughes C, Fanslow W, Pober J. Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA‐3 signaling pathway. European Journal Of Immunology 1996, 26: 610-617. PMID: 8605928, DOI: 10.1002/eji.1830260316.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, Differentiation, T-LymphocyteB-LymphocytesCD2 AntigensCD40 LigandCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell CommunicationCells, CulturedEndothelium, VascularHumansInterleukin-2LigandsLymphocyte ActivationMembrane GlycoproteinsPhytohemagglutininsSignal TransductionUp-RegulationConceptsCD40 ligand expressionExogenous IL-2T cellsIL-2Ligand expressionCD2/LFACD40 ligandHuman peripheral blood CD4Peripheral blood adherent cellsHelper T cell subsetsPeripheral blood T cellsPeripheral blood CD4T cell subsetsCo-stimulatory moleculesBlood T cellsHelper T cellsIL-2 receptorCo-stimulatory signalsIL-2 secretionInterleukin-2 secretionBlood CD4Endothelial effectsCell subsetsHuman CD4B cells
1991
Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells
Savage C, Hughes C, Pepinsky R, Wallner B, Freedman A, Pober J. Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells. Cellular Immunology 1991, 137: 150-163. PMID: 1679377, DOI: 10.1016/0008-8749(91)90065-j.Peer-Reviewed Original ResearchConceptsLymphocyte function-associated antigen-3Adhesion molecule-1T cellsEndothelial cellsMolecule-1Antigen 3Human peripheral blood CD4Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Peripheral blood CD4T cell populationsB7/BB1Cultured human endothelial cellsIL-2 synthesisBlood CD4EC costimulationHuman endothelial cellsT cell CD2IL-2CD4CostimulationCell populationsComparable degreeLines of evidence
1990
Lymphocyte-mediated activation of cultured endothelial cells (EC). CD4+ T cells inhibit EC class II MHC expression despite secreting IFN-gamma and increasing EC class I MHC and intercellular adhesion molecule-1 expression.
Doukas J, Pober JS. Lymphocyte-mediated activation of cultured endothelial cells (EC). CD4+ T cells inhibit EC class II MHC expression despite secreting IFN-gamma and increasing EC class I MHC and intercellular adhesion molecule-1 expression. The Journal Of Immunology 1990, 145: 1088-98. PMID: 1974272, DOI: 10.4049/jimmunol.145.4.1088.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteCD4 AntigensCD4-Positive T-LymphocytesCD8 AntigensCell Adhesion MoleculesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansImmune SeraInterferon-gammaPhytohemagglutininsT-Lymphocytes, RegulatoryConceptsClass II MHC expressionIntercellular adhesion molecule-1 expressionClass I MHCAdhesion molecule-1 expressionClass II AgClass II expressionMolecule-1 expressionT cellsMHC expressionIFN-gammaI MHCEndothelial cellsICAM-1Class II MHC inductionClass IClass II MHC AgCoculture of CD4Failure of CD4Immunoreactive IFN-gammaICAM-1 expressionClass II MHCCultured endothelial cellsUnfractionated PBLTime of inductionMHC induction
1989
Vascular endothelial cells enhance T cell responses by markedly augmenting IL-2 concentrations
Guinan E, Smith B, Doukas J, Miller R, Pober J. Vascular endothelial cells enhance T cell responses by markedly augmenting IL-2 concentrations. Cellular Immunology 1989, 118: 166-177. PMID: 2562928, DOI: 10.1016/0008-8749(89)90366-3.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsIL-2 concentrationsPolyclonal mitogen phytohemagglutininT cell responsesEndothelial cellsBlood monocytesUnfractionated peripheral blood mononuclear cellsCell responsesPrimary allogeneic responseSuboptimal PHA concentrationsExogenous IL-2Blood mononuclear cellsT cell populationsRecombinant IL-1Cultured human endothelial cellsDermal fibroblastsIL-2 synthesisVascular endothelial cellsQuantity of antigenHuman endothelial cellsAllogeneic responseMitogen phytohemagglutininMononuclear cellsIL-2Presence of EC
1983
Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon.
Pober JS, Gimbrone MA, Cotran RS, Reiss CS, Burakoff SJ, Fiers W, Ault KA. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. Journal Of Experimental Medicine 1983, 157: 1339-1353. PMID: 6403654, PMCID: PMC2186979, DOI: 10.1084/jem.157.4.1339.Peer-Reviewed Original ResearchConceptsAllogeneic T cellsT cellsIa antigensEndothelial cellsPeripheral blood mononuclear cellsBlood mononuclear cellsCultured human umbilical vein endothelial cellsExpression of IaActivated T cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsAllograft rejectionVein endothelial cellsGamma interferon geneFluorescence flow cytometryHuman gamma interferonMononuclear cellsImmune responseGamma interferonVascular endotheliumIa expressionFlow cytometryImmune interferonMonoclonal antibodiesAntigen
1982
Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation.
Pober JS, Gimbrone MA. Expression of Ia-like antigens by human vascular endothelial cells is inducible in vitro: demonstration by monoclonal antibody binding and immunoprecipitation. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 6641-6645. PMID: 6815654, PMCID: PMC347184, DOI: 10.1073/pnas.79.21.6641.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalCell DivisionCells, CulturedEndotheliumGene Expression RegulationGenes, MHC Class IIHumansPhytohemagglutininsUmbilical VeinsConceptsHuman vascular endothelial cellsCell shape changesVascular endothelial cellsEndothelial cellsMembrane proteinsCell divisionRadioiodinated membrane proteinsHuman endothelial cellsStandard culture conditionsIntact cellsCultured human endothelial cellsBiochemical demonstrationImmunoprecipitationCulture conditionsConfluent culturesExpressionMonoclonal antibody bindingCellsPrimary culturesShape changesMonoclonal antibodiesLectin phytohemagglutininProteinRegulationImportant implications