2013
The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule
Capasso G, Geibel PJ, Damiano S, Jaeger P, Richards WG, Geibel JP. The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule. Kidney International 2013, 84: 277-284. PMID: 23615500, DOI: 10.1038/ki.2013.137.Peer-Reviewed Original ResearchMeSH KeywordsAcid-Base EquilibriumAnimalsCalcimimetic AgentsCalciumHydrogen-Ion ConcentrationIn Vitro TechniquesKidney Tubules, ProximalMaleMiceMice, KnockoutNuclear ProteinsPerfusionPuncturesRatsRats, Sprague-DawleyReceptors, Calcium-SensingReceptors, G-Protein-CoupledSodium-Hydrogen ExchangersTranscription FactorsConceptsProximal tubulesLuminal calcium concentrationFluid reabsorptionAcid secretionKnockout miceCalcium ion concentrationCalcium concentrationRole of CaSRActivation of CaSRCalcium sensing receptorCalcium-sensing receptorMouse proximal tubulesProton secretionSodium-hydrogen exchangerCalcimimetic agentBicarbonate absorptionSensing receptorCaSRFluid absorptionSecretionReabsorptionMiceCalcimimeticsTubulesLuminal membrane
2008
Mouse model of type II Bartter's syndrome. II. Altered expression of renal sodium- and water-transporting proteins
Wagner CA, Loffing-Cueni D, Yan Q, Schulz N, Fakitsas P, Carrel M, Wang T, Verrey F, Geibel JP, Giebisch G, Hebert SC, Loffing J. Mouse model of type II Bartter's syndrome. II. Altered expression of renal sodium- and water-transporting proteins. American Journal Of Physiology. Renal Physiology 2008, 294: f1373-f1380. PMID: 18322017, DOI: 10.1152/ajprenal.00613.2007.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAnimalsBartter SyndromeCarrier ProteinsCation Transport ProteinsDinoprostoneDisease Models, AnimalEpithelial Sodium ChannelsKidney Tubules, DistalKidney Tubules, ProximalLoop of HenleMiceMice, Mutant StrainsPotassium Channels, Inwardly RectifyingReverse Transcriptase Polymerase Chain ReactionSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSodium-Phosphate Cotransporter Proteins, Type IIaSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 1Up-RegulationWaterConceptsThick ascending limbBartter's syndromeBartter-like phenotypeType II Bartter's syndromeWild-type miceAntenatal Bartter syndromeWild-type littermatesROMK null micePlasma aldosteroneMaternal polyhydramniosRenal sodiumVolume depletionRenal tubulopathyMouse modelSemiquantitative immunoblottingProximal tubulesAscending limbKidney homogenatesSyndromeHenle's loopNull miceDCT cellsWater transport proteinsCompensatory mechanismsMice
2003
Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells
Lang K, Wagner C, Haddad G, Burnekova O, Geibel J. Intracellular pH Activates Membrane-Bound Na+/H+ Exchanger and Vacuolar H+-ATPase in Human Embryonic Kidney (HEK) Cells. Cellular Physiology And Biochemistry 2003, 13: 257-262. PMID: 14586169, DOI: 10.1159/000074540.Peer-Reviewed Original ResearchConceptsMicroM EIPAIntracellular acidificationFree bath solutionProximal tubule cellsInfluence of intracellularWestern blot analysisTime-dependent mannerTubule cellsHuman embryonic kidney cellsHEK-293 cellsExtruding mechanismHuman embryonic kidney cell line HEK-293NHE3 proteinEmbryonic kidney cellsCell line HEK-293Cytosolic pHKidney cellsBlot analysisAbsence of bicarbonateHEK293 cellsBath solutionEIPAPresent studyActivationCells
2001
K+-induced HSP-72 expression is mediated via rapid Ca2+ influx in renal epithelial cells
Eickelberg O, Geibel J, Seebach F, Giebisch G, Kashgarian M. K+-induced HSP-72 expression is mediated via rapid Ca2+ influx in renal epithelial cells. American Journal Of Physiology. Renal Physiology 2001, 281: f280-f287. PMID: 11457719, DOI: 10.1152/ajprenal.2001.281.2.f280.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCell LineDiltiazemEgtazic AcidEnzyme InhibitorsEpithelial CellsGallic AcidGenes, ReporterHeat-Shock ProteinsHSP72 Heat-Shock ProteinsImmunoblottingKidney Tubules, ProximalMicroscopy, ConfocalPotassiumPromoter Regions, GeneticRecombinant Fusion ProteinsSodiumSwineThapsigarginUrotheliumConceptsHSP 72 expressionPromoter activityHSP 72Protein expressionProtective cellular responseLuciferase reporter geneHSP-25Heat shock protein expressionRenal epithelial cellsTranscriptional inductionShock protein expressionIonic stressReporter geneHSP-90 levelsHSC 73Cellular responsesChannel blocker diltiazemIntracellular lumenWestern blot analysisChelator EGTA-AMPathophysiological stimuliBlot analysisConfocal microscopyProtein levelsExtracellular space
2000
Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−
Malnic G, Geibel J. Cell pH and H+ Secretion by S3 Segment of Mammalian Kidney: Role of H+-ATPase and Cl−. The Journal Of Membrane Biology 2000, 178: 115-125. PMID: 11083900, DOI: 10.1007/s002320010019.Peer-Reviewed Original ResearchConceptsProximal tubule cellsChannel blocker NPPBS3 segmentTubule cellsProximal S3 segmentsFree mediumCell pHConfocal microscopyNIH Image programCell pH regulationMicroperfusion techniqueSuperfusionMouse kidneyRabbit kidneyKidneySubcellular vesiclesApical areaCell acidificationMammalian kidneyFluorescence densityNPPBRingerCellsImage programNaCl Ringer
1998
Angiotensin II stimulates vesicular H+-ATPase in rat proximal tubular cells
Wagner C, Giebisch G, Lang F, Geibel J. Angiotensin II stimulates vesicular H+-ATPase in rat proximal tubular cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 9665-9668. PMID: 9689138, PMCID: PMC21396, DOI: 10.1073/pnas.95.16.9665.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsEnzyme ActivationHydrogen-Ion ConcentrationIon TransportKidney Tubules, ProximalMicrovilliProton-Translocating ATPasesRatsRats, Sprague-DawleySodiumConceptsAngiotensin IIProximal tubulesIon secretionEffect of AIIRat proximal tubular cellsAngiotensin II stimulatesRemoval of ClPH-sensitive fluorescent dye 2Proximal tubular cellsNa/H exchangerBafilomycin A1Proximal tubule fragmentsTubular cellsBicarbonate reabsorptionHCO3- reabsorptionTubule fragmentsH exchangerATPase activityFluorescent dye 2Proton pump inhibitor bafilomycin A1Intracellular pHTubulesSecretionPresent studyCell pH
1990
Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule.
Geibel J, Giebisch G, Boron W. Angiotensin II stimulates both Na(+)-H+ exchange and Na+/HCO3- cotransport in the rabbit proximal tubule. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 7917-7920. PMID: 2172967, PMCID: PMC54862, DOI: 10.1073/pnas.87.20.7917.Peer-Reviewed Original ResearchConceptsAngiotensin IIHCO3- reabsorptionProximal tubulesEffect of AIIS1 proximal tubulesNM angiotensin IIPH-sensitive fluorescent dye 2Rat proximal tubuleRabbit proximal tubuleSuperficial nephronsPotent stimulusBasolateral administrationRabbit kidneyFluorescent dye 2Basolateral solutionBasolateral stepTransporter activityTubulesIonic mechanismsCotransportKidneyAdministrationNephron
1989
Basolateral sodium-coupled acid-base transport mechanisms of the rabbit proximal tubule
Geibel J, Giebisch G, Boron W. Basolateral sodium-coupled acid-base transport mechanisms of the rabbit proximal tubule. American Journal Of Physiology 1989, 257: f790-f797. PMID: 2556038, DOI: 10.1152/ajprenal.1989.257.5.f790.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAcid-Base EquilibriumAnimalsBiological TransportBiomechanical PhenomenaCarrier ProteinsChloridesDrug ResistanceHydrogen-Ion ConcentrationKidney Tubules, ProximalRabbitsSodiumSodium-Hydrogen ExchangersEffects of acetate on luminal acidification processes in the S3 segment of the rabbit proximal tubule
Geibel J, Giebisch G, Boron W. Effects of acetate on luminal acidification processes in the S3 segment of the rabbit proximal tubule. American Journal Of Physiology 1989, 257: f586-f594. PMID: 2801961, DOI: 10.1152/ajprenal.1989.257.4.f586.Peer-Reviewed Original Research
1984
A microelectrode for continuous monitoring of glucose concentration in isolated perfused tubule segments
Rehwald W, Geibel J, Gstrein E, Oberleithner H. A microelectrode for continuous monitoring of glucose concentration in isolated perfused tubule segments. Pflügers Archiv - European Journal Of Physiology 1984, 400: 398-402. PMID: 6462888, DOI: 10.1007/bf00587539.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalibrationGlucoseIn Vitro TechniquesKidney TubulesKidney Tubules, ProximalMiceMice, Inbred StrainsMicroelectrodesMonitoring, PhysiologicPerfusionTemperatureA microelectrode for continuous recording of volume fluxes in isolated perfused tubule segments
Geibel J, Völkl H, Lang F. A microelectrode for continuous recording of volume fluxes in isolated perfused tubule segments. Pflügers Archiv - European Journal Of Physiology 1984, 400: 388-392. PMID: 6462886, DOI: 10.1007/bf00587537.Peer-Reviewed Original ResearchAnimalsGalactose OxidaseKidney TubulesKidney Tubules, ProximalMiceMicroelectrodesPerfusionTemperatureA microelectrode for continuous monitoring of redox activity in isolated perfused tubule segments
Völkl H, Geibel J, Rehwald W, Lang F. A microelectrode for continuous monitoring of redox activity in isolated perfused tubule segments. Pflügers Archiv - European Journal Of Physiology 1984, 400: 393-397. PMID: 6462887, DOI: 10.1007/bf00587538.Peer-Reviewed Original Research