2021
The ALT upper reference interval debate: Blame it on the alcohol
Vera MA, Koch CD, Liapakis A, Lim JK, El-Khoury JM. The ALT upper reference interval debate: Blame it on the alcohol. Clinica Chimica Acta 2021, 526: 62-65. PMID: 34973184, DOI: 10.1016/j.cca.2021.12.026.Peer-Reviewed Original ResearchConceptsUpper reference limitAdult groupHigher upper reference limitLiver diseasePediatric groupPediatric GastroenterologyPediatric populationClinical guidelinesALT valuesAmerican CollegeDerivation studyAge groupsReference limitsNorth American SocietyAmerican SocietyClinical laboratoriesRoche Cobas 8000GastroenterologyLaboratory expertsWomenCobas 8000MenGroupVariable inclusionIndividualsDetermination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population
Vera MA, Koch CD, Kavsak PA, El-Khoury JM. Determination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population. Clinica Chimica Acta 2021, 523: 397-401. PMID: 34666029, DOI: 10.1016/j.cca.2021.10.011.Peer-Reviewed Original ResearchConceptsHeart-type fatty acid-binding proteinUpper reference limitPercentile upper reference limitClinical utilityReference limitsAcute kidney injuryHeart-type fatty acidAcute coronary syndromeAACC Universal Sample BankFatty acid-binding proteinH-FABP concentrationHealthy U.S.Coronary syndromeFinal sample populationKidney injuryAssay cutoffHealthy donorsAcid-binding proteinUS populationImmunoturbidimetric assayPotential biomarkersClinical sensitivityMyocardial tissueSerum samplesLow molecular weight proteins
2019
The Role of Volume Regulation and Thermoregulation in AKI during Marathon Running
Mansour SG, Martin TG, Obeid W, Pata RW, Myrick KM, Kukova L, Jia Y, Bjornstad P, El-Khoury JM, Parikh CR. The Role of Volume Regulation and Thermoregulation in AKI during Marathon Running. Clinical Journal Of The American Society Of Nephrology 2019, 14: 1297-1305. PMID: 31413064, PMCID: PMC6730516, DOI: 10.2215/cjn.01400219.Peer-Reviewed Original ResearchConceptsCore body temperatureBody temperaturePercent of runnersAcute tubular injuryPlasma copeptin concentrationsMajority of runnersContinuous core body temperatureAKIN criteriaDays postmarathonPrior marathonsTransient AKICopeptin concentrationsTubular injuryInjury biomarkersPrimary outcomeProspective cohortNephron damageNegative sodiumUrine microscopyVolume lossUrine sedimentAKIAbstractTextSweat sodiumCopeptin
2016
Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function
El-Khoury JM, Bunch DR, Hu B, Payto D, Reineks EZ, Wang S. Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function. Clinical Biochemistry 2016, 49: 1140-1143. PMID: 27452178, DOI: 10.1016/j.clinbiochem.2016.07.009.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateKidney functionCystatin CEGFR equationsWidespread clinical useIothalamate clearanceEGFR formulasFiltration rateSymmetric dimethylargininePlasma specimensAdult populationStrong markerAbstractTextClinical useEndogenous markerCreatinineLiquid chromatography-tandem mass spectrometry methodChromatography-tandem mass spectrometry methodFurther studiesDimethylarginineRoche Cobas 8000MarkersCatabolic productsCobas 8000
2011
A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized l-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges
El-Khoury JM, Bunch DR, Reineks E, Jackson R, Steinle R, Wang S. A simple and fast liquid chromatography–tandem mass spectrometry method for measurement of underivatized l-arginine, symmetric dimethylarginine, and asymmetric dimethylarginine and establishment of the reference ranges. Analytical And Bioanalytical Chemistry 2011, 402: 771-779. PMID: 22124751, DOI: 10.1007/s00216-011-5462-9.Peer-Reviewed Original ResearchConceptsFast liquid chromatography–tandem mass spectrometry methodAnalytical cycle timeChromatography-tandem mass spectrometry methodLiquid chromatography-tandem mass spectrometry methodMinimum sample preparationMass spectrometry methodLiquid chromatography-tandem mass spectrometryChromatography-tandem mass spectrometryAsymmetric dimethylarginineSymmetric dimethylarginineLC-MS/MS methodMass spectrometerFormic acidSpectrometry methodMass spectrometrySilica columnProtein precipitationSample preparationInternal standardAmmonium acetateMS methodGlomerular filtration rateAnalytesNitric oxide synthaseΜM