2023
Rapid serum tubes reduce transport hemolysis and false positive rates for high-sensitivity troponin T
Malaeb H, Vera M, Sangal R, Venkatesh A, Possick S, Maciejak L, Oberle E, El-Khoury J. Rapid serum tubes reduce transport hemolysis and false positive rates for high-sensitivity troponin T. Clinica Chimica Acta 2023, 551: 117630. PMID: 37931733, DOI: 10.1016/j.cca.2023.117630.Peer-Reviewed Original ResearchRapid serum tubes reduce transport hemolysis and false positive rates for high-sensitivity troponin T.
Malaeb H, Vera M, Sangal R, Venkatesh A, Possick S, Maciejak L, Oberle E, El-Khoury J. Rapid serum tubes reduce transport hemolysis and false positive rates for high-sensitivity troponin T. Clinica Chimica Acta 2023, 551: 117630. PMID: 38420909, DOI: 10.1016/j.cca.2023.117630.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersBlood Specimen CollectionEmergency Service, HospitalHemolysisHumansPlasmaSerumTroponin TConceptsRapid Serum TubesPlasma separation tubesHigh-sensitivity troponin THs-cTnT valuesHs-cTnTSource of hemolysisEmergency departmentTroponin TSerum tubesFalse positive resultsMedian reductionEffect of hemolysisED patientsSample collectionEffects of tube typeHemolysisPatientsPositive resultsSeparator tubes
2022
Urinary Biomarkers of Tubular Health and Risk for Kidney Function Decline or Mortality in Diabetes
Chen T, Coca S, Thiessen-Philbrook H, Heerspink H, Obeid W, Ix J, Fried L, Bonventre J, El-Khoury J, Shlipak M, Parikh C. Urinary Biomarkers of Tubular Health and Risk for Kidney Function Decline or Mortality in Diabetes. American Journal Of Nephrology 2022, 53: 775-785. PMID: 36630924, PMCID: PMC10006337, DOI: 10.1159/000528918.Peer-Reviewed Original ResearchConceptsKidney function declineEnd-stage kidney diseaseFunction declineMCP-1Tubular healthUrinary biomarkersEGFR declineKIM-1YKL-40High riskChronic kidney disease progressionSubsequent eGFR declineKidney disease progressionGlomerular filtration rateYKL-40 levelsAvailable urine samplesOne-year changeHigher baseline levelsSubset of participantsBaseline UACRMedian UACRCause mortalityUrine albuminTubular injuryCreatinine ratio
2021
Determination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population
Vera MA, Koch CD, Kavsak PA, El-Khoury JM. Determination of 97.5th and 99th percentile upper reference limits for heart-type fatty acid-binding protein (H-FABP) in a US population. Clinica Chimica Acta 2021, 523: 397-401. PMID: 34666029, DOI: 10.1016/j.cca.2021.10.011.Peer-Reviewed Original ResearchConceptsHeart-type fatty acid-binding proteinUpper reference limitPercentile upper reference limitClinical utilityReference limitsAcute kidney injuryHeart-type fatty acidAcute coronary syndromeAACC Universal Sample BankFatty acid-binding proteinH-FABP concentrationHealthy U.S.Coronary syndromeFinal sample populationKidney injuryAssay cutoffHealthy donorsAcid-binding proteinUS populationImmunoturbidimetric assayPotential biomarkersClinical sensitivityMyocardial tissueSerum samplesLow molecular weight proteinsAACC Guidance Document on Laboratory Investigation of Acute Kidney Injury
El-Khoury JM, Hoenig MP, Jones GRD, Lamb EJ, Parikh CR, Tolan NV, Wilson FP. AACC Guidance Document on Laboratory Investigation of Acute Kidney Injury. The Journal Of Applied Laboratory Medicine 2021, 6: 1316-1337. PMID: 33973621, DOI: 10.1093/jalm/jfab020.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsAcute kidney injuryKidney injuryNew biomarkersPotential acute kidney injuryInsulin-like growth factor binding protein 7Growth factor binding protein 7Early detectionLong-term complicationsLaboratory investigationsEmergence of biomarkersHealth care costsBinding protein 7AKI alertsKidney damageClinical indicationsCystatin CSudden episodeCare costsTissue inhibitorDiagnostic thresholdMetalloproteinases 2Test utilizationPreponderance of evidenceTraditional markersExpert recommendationsIs It Time to Move On? Reexamining Race in Glomerular Filtration Rate Equations
Moderators:, El-Khoury JM, Ovalle AA, Cervinski MA, Experts:, Levey AS, Jones G, Eneanya ND, Lieske JC, Jha V. Is It Time to Move On? Reexamining Race in Glomerular Filtration Rate Equations. Clinical Chemistry 2021, 67: 585-591. PMID: 33686430, DOI: 10.1093/clinchem/hvaa333.Peer-Reviewed Original Research
2020
Uromodulin to Osteopontin Ratio in Deceased Donor Urine Is Associated With Kidney Graft Outcomes.
Mansour SG, Liu C, Jia Y, Reese PP, Hall IE, El-Achkar TM, LaFavers KA, Obeid W, Rosenberg AZ, Daneshpajouhnejad P, Doshi MD, Akalin E, Bromberg JS, Harhay MN, Mohan S, Muthukumar T, Schröppel B, Singh P, El-Khoury JM, Weng FL, Thiessen-Philbrook HR, Parikh CR. Uromodulin to Osteopontin Ratio in Deceased Donor Urine Is Associated With Kidney Graft Outcomes. Transplantation 2020, 105: 876-885. PMID: 32769629, PMCID: PMC8805736, DOI: 10.1097/tp.0000000000003299.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsBiomarkersCells, CulturedDelayed Graft FunctionFemaleGlomerular Filtration RateGraft SurvivalHistocompatibility Antigens Class IIHumansKidneyKidney TransplantationMacrophagesMaleMiceMiddle AgedOsteopontinProspective StudiesRisk AssessmentRisk FactorsTime FactorsTissue DonorsUnited StatesUromodulinConceptsGraft failureGraft functionGraft outcomePrimary outcomeMajor histocompatibilityDeath-censored graft failureDeceased donor kidney transplantationCause graft failureImproved graft survivalRecipient graft outcomesKidney graft outcomeGlomerular filtration rateDeceased donor kidneysTime of procurementGraft survivalKidney transplantationDirection of associationDeceased donorsFiltration rateKidney qualityLower riskExtensive injuryDonor urineSimilar associationMouse macrophages
2019
Comparison of Urine and Plasma Biomarker Concentrations Measured by Aptamer-Based versus Immunoassay Methods in Cardiac Surgery Patients
Kukova LZ, Mansour SG, Coca SG, de Fontnouvelle CA, Thiessen-Philbrook HR, Shlipak MG, El-Khoury JM, Parikh CR. Comparison of Urine and Plasma Biomarker Concentrations Measured by Aptamer-Based versus Immunoassay Methods in Cardiac Surgery Patients. The Journal Of Applied Laboratory Medicine 2019, 4: 331-342. PMID: 31659071, DOI: 10.1373/jalm.2018.028621.Peer-Reviewed Original ResearchConceptsCardiac surgery patientsSurgery patientsCardiac surgeryBiomarker concentrationsAcute kidney injuryComparison of urinePlasma biomarker concentrationsImmunoassay methodPostoperative biomarkersPostoperative plasmaPreoperative biomarkersKidney injuryInjury cohortBiomarker discoveryBiomarker endpointsClinical settingCurrent immunoassay methodsBiomarker valuesUrine samplesTranslational researchUrineSpearman correlationPaired samplesPatientsSurgery
2018
Emerging Biomarker of Kidney Disease: suPAR or Subpar?
Bunch DR, El-Khoury JM. Emerging Biomarker of Kidney Disease: suPAR or Subpar? Clinical Chemistry 2018, 64: 1545-1547. PMID: 32100860, DOI: 10.1373/clinchem.2018.293167.Peer-Reviewed Original Research
2017
Urinalysis findings and urinary kidney injury biomarker concentrations
Nadkarni GN, Coca SG, Meisner A, Patel S, Kerr KF, Patel UD, Koyner JL, Garg AX, Philbrook HT, Edelstein CL, Shlipak M, El-Khoury J, Parikh CR, on behalf of the TRIBE-AKI Consortium Investigators. Urinalysis findings and urinary kidney injury biomarker concentrations. BMC Nephrology 2017, 18: 218. PMID: 28683730, PMCID: PMC5499057, DOI: 10.1186/s12882-017-0629-z.Peer-Reviewed Original ResearchConceptsKidney injury molecule-1Post-operative samplesKidney injuryUrinalysis findingsUrinary biomarkersBiomarker concentrationsPost-operative AKIPre-operative samplesUrine dipstick testingPost-operative time pointsInjury molecule-1Time pointsRenal tubular damageUrinary biomarker concentrationsUrinary biomarker levelsTRIBE-AKI cohortLiver fatty acidClinical AKIDipstick proteinuriaProteinuria concentrationsUrinary NGALAKI biomarkersTubular damageCardiac surgeryNeutrophil gelatinase
2016
Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function
El-Khoury JM, Bunch DR, Hu B, Payto D, Reineks EZ, Wang S. Comparison of symmetric dimethylarginine with creatinine, cystatin C and their eGFR equations as markers of kidney function. Clinical Biochemistry 2016, 49: 1140-1143. PMID: 27452178, DOI: 10.1016/j.clinbiochem.2016.07.009.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateKidney functionCystatin CEGFR equationsWidespread clinical useIothalamate clearanceEGFR formulasFiltration rateSymmetric dimethylargininePlasma specimensAdult populationStrong markerAbstractTextClinical useEndogenous markerCreatinineLiquid chromatography-tandem mass spectrometry methodChromatography-tandem mass spectrometry methodFurther studiesDimethylarginineRoche Cobas 8000MarkersCatabolic productsCobas 8000
2014
Recent advances in MS methods for nicotine and metabolite analysis in human matrices: clinical perspectives
El-Khoury JM, Wang S. Recent advances in MS methods for nicotine and metabolite analysis in human matrices: clinical perspectives. Bioanalysis 2014, 6: 2171-2183. PMID: 25331861, DOI: 10.4155/bio.14.176.Peer-Reviewed Original ResearchConceptsHuman matricesMajor global health issueSecond-hand exposureGlobal health issueTobacco smokingPreventable deathsTobacco consumptionAppropriate biomarkersHand exposureClinical MSNicotine metabolismClinical perspectiveTobacco productsHealth issuesNicotineMeasurement of nicotineMetabolitesStandard reference methodDetection of nicotineSmokingMetabolite analysisBiomarkersSuperior sensitivity