2016
Effects of andrographolide on intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats
Khamphaya T, Chansela P, Piyachaturawat P, Suksamrarn A, Nathanson MH, Weerachayaphorn J. Effects of andrographolide on intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats. European Journal Of Pharmacology 2016, 789: 254-264. PMID: 27475677, PMCID: PMC10804355, DOI: 10.1016/j.ejphar.2016.07.032.Peer-Reviewed Original ResearchConceptsCholestatic liver diseaseLiver diseaseIntrahepatic cholestasisLiver injuryProtective effectHepatic stellate cell activationAcute intrahepatic cholestasisAlpha-smooth muscle actinCholestatic liver injuryBile duct proliferationSerum alanine aminotransferaseNF-κB expressionSingle intraperitoneal injectionEffects of andrographolidePromising therapeutic optionEffective therapeutic approachPotent protective propertiesNuclear factor kappaStellate cell activationANIT injectionDuct proliferationTherapeutic optionsHepatoprotective effectPeriductular fibrosisAlternative therapies
2013
Alleviative effect of andrographolide on alpha‐naphthylisothiocyanate‐induced cholestatic liver injury in rats
Khamphaya T, Piyachaturawat P, Weerachayaphorn J. Alleviative effect of andrographolide on alpha‐naphthylisothiocyanate‐induced cholestatic liver injury in rats. The FASEB Journal 2013, 27: 1161.2-1161.2. DOI: 10.1096/fasebj.27.1_supplement.1161.2.Peer-Reviewed Original ResearchLiver injuryMonocyte chemoattractant protein-1Severe liver inflammationCholestatic liver damageCholestatic liver injuryBile duct proliferationChemoattractant protein-1Tumor necrosis factorPotential therapeutic actionNF-kB signalingGamma-glutamyl transpeptidaseLiver inflammationSerum aminotransferasesLiver failureLiver diseaseDuct proliferationLiver damageHepatoprotective effectMajor active compoundNecrosis factorHepatic accumulationNF-kBAndrographolide pretreatmentANITBile acids
2010
Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression
Xu S, Weerachayaphorn J, Cai SY, Soroka CJ, Boyer JL. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. AJP Gastrointestinal And Liver Physiology 2010, 299: g126-g135. PMID: 20395535, PMCID: PMC2904108, DOI: 10.1152/ajpgi.00522.2010.Peer-Reviewed Original ResearchMeSH Keywords5' Flanking RegionAnimalsAryl Hydrocarbon Receptor Nuclear TranslocatorBase SequenceBasic Helix-Loop-Helix Transcription FactorsBinding SitesButylaminesChromatin ImmunoprecipitationDose-Response Relationship, DrugElectrophoretic Mobility Shift AssayGenes, ReporterHep G2 CellsHepatocytesHumansMethylcholanthreneMiceMolecular Sequence DataMultidrug Resistance-Associated ProteinsMutationNF-E2-Related Factor 2Polychlorinated DibenzodioxinsPromoter Regions, GeneticPyrazinesReceptors, Aryl HydrocarbonResponse ElementsRNA InterferenceRNA, MessengerThionesThiophenesTime FactorsTransfectionConceptsMRP4 expressionAryl hydrocarbon receptorNF-E2Renal proximal tubule epitheliumCholestatic liver injuryHydrocarbon receptorXenobiotic response elementProximal tubule epitheliumMultidrug resistance protein 4Factor 2Bile salt conjugatesNuclear receptor agonistsLiver injuryPromoter activityReceptor agonistTherapeutic benefitAnimal modelsNrf2 activatorsTubule epitheliumHuman MRP4Conjugated steroidsNuclear factorAdaptive upregulationOxidative stressLuciferase reporter
2009
Nuclear factor erythroid 2–related factor 2 is a positive regulator of human bile salt export pump expression
Weerachayaphorn J, Cai S, Soroka CJ, Boyer JL. Nuclear factor erythroid 2–related factor 2 is a positive regulator of human bile salt export pump expression. Hepatology 2009, 50: 1588-1596. PMID: 19821532, PMCID: PMC3013376, DOI: 10.1002/hep.23151.Peer-Reviewed Original ResearchMeSH KeywordsATP Binding Cassette Transporter, Subfamily B, Member 11ATP-Binding Cassette TransportersBase SequenceHep G2 CellsHepatocytesHumansMaf Transcription FactorsMolecular Sequence DataNF-E2-Related Factor 2PyrazinesReverse Transcriptase InhibitorsRNA, MessengerSignal TransductionThionesThiophenesConceptsBile salt export pumpCholestatic liver injuryPositive transcriptional regulatorElectrophoretic mobility shift assaysHepG2 cellsBSEP expressionChromatin immunoprecipitation assaysBSEP promoterMobility shift assaysAdditional transcriptional factorsProximal promoter regionBSEP promoter activitySmall interfering RNAsFactor 2Liver injuryNuclear factorAntioxidant responsive elementTranscriptional regulatorsNrf2 transcriptional activationTranscriptional activationPositive regulatorBile salt export pump expressionNuclear farnesoid X receptorImmunoprecipitation assaysShift assays