2005
Human Monocytotropic Ehrlichiosis
Dawson J, Ewing S, Davidson W, Childs J, Little S, Standaert S. Human Monocytotropic Ehrlichiosis. 2005, 239-257. DOI: 10.1128/9781555816490.ch14.Peer-Reviewed Original ResearchHuman monocytotropic ehrlichiosisMonocytotropic ehrlichiosisMild self-limiting symptomsSelf-limiting symptomsNonspecific febrile illnessSpectrum of manifestationsSeasonality of transmissionTick-borne zoonosisFebrile illnessAsymptomatic infectionClinical recognitionHigh riskRickettsial infectionEpidemiological studiesEtiologic agentFatal diseaseLaboratory diagnosisZoonotic diseaseEhrlichia chaffeensisLone star tickE. chaffeensisInfectionDiseaseEhrlichiosisUnited States
2001
Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in Persons Coinfected with Human Immunodeficiency Virus
Paddock C, Folk S, Shore G, Machado L, Huycke M, Slater L, Liddell A, Buller R, Storch G, Monson T, Rimland D, Sumner J, Singleton J, Bloch K, Tang Y, Standaert S, Childs J. Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in Persons Coinfected with Human Immunodeficiency Virus. Clinical Infectious Diseases 2001, 33: 1586-1594. PMID: 11568857, DOI: 10.1086/323981.Peer-Reviewed Original ResearchConceptsHuman immunodeficiency virusImmunodeficiency virusE. ewingiiE. chaffeensisCases of ehrlichiosisDiagnosis of ehrlichiosisT-lymphocyte countsCells/microL.Ehrlichia ewingiiEhrlichia chaffeensisLife-threatening illnessLymphocyte countClinical courseHIV infectionMedical managementPatient cohortSevere manifestationsPatientsLaboratory evaluationEhrlichiosisChaffeensisInfectionEwingiiMultiple factorsVirus
2000
DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON
Comer J, Nicholson W, Paddock C, Sumner J, Childs J. DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON. Journal Of Wildlife Diseases 2000, 36: 705-712. PMID: 11085432, DOI: 10.7589/0090-3558-36.4.705.Peer-Reviewed Original ResearchSurvival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs
McKechnie D, Slater K, Childs J, Massung R, Paddock C. Survival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs. Transfusion 2000, 40: 1041-1047. PMID: 10988303, DOI: 10.1046/j.1537-2995.2000.40091041.x.Peer-Reviewed Original ResearchConceptsE. chaffeensisNucleated cellsTransfusion-acquired infectionEhrlichia chaffeensisMonocytic cell line THP-1Cell line THP-1Infected cell linesCytocentrifuged slidesTransfusion transmissionPacked RBC unitsPCR positivityE. chaffeensis DNASupernatant fractionCell culturesWeekly examinationsRBC unitsStudy designRBC productsTHP-1DH82 cellsPCR resultsCell linesChaffeensisInfectionSuccessful reisolationPrimary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics
Standaert S, Yu T, Scott M, Childs J, Paddock C, Nicholson W, Singleton J, Blaser M. Primary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics. The Journal Of Infectious Diseases 2000, 181: 1082-1088. PMID: 10720534, DOI: 10.1086/315346.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionE. chaffeensisCerebrospinal fluid specimensCultures of bloodHigh diagnostic yieldEhrlichia chaffeensisPrimary isolationFebrile illnessDiagnostic titerBlood isolatesDiagnostic yieldTick exposureSame patientCerebrospinal fluidPatientsFluid specimensDiagnostic testsFrequent isolationBloodChain reactionChaffeensisInfectionMolecular characteristicsMolecular analysisIsolates
1999
Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation
Sumner J, Childs J, Paddock C. Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation. Journal Of Clinical Microbiology 1999, 37: 1447-1453. PMID: 10203503, PMCID: PMC84798, DOI: 10.1128/jcm.37.5.1447-1453.1999.Peer-Reviewed Original ResearchConceptsOpen reading frameAmino acid sequenceApparent molecular massE. chaffeensisGenomic libraryMolecular cloningReading frameAcid sequenceDifferentiation of isolatesPCR targetsClose homologyMolecular massNucleotide sequencingImmunoreactive proteinDifferent isolatesDNA samplesInterstrain variationGenesRepeat unitsMolecular databasesCell culturesAmblyomma americanum ticksSequencePCR detectionChaffeensisSerologic Testing for Human Granulocytic Ehrlichiosis at a National Referral Center
Comer J, Nicholson W, Olson J, Childs J. Serologic Testing for Human Granulocytic Ehrlichiosis at a National Referral Center. Journal Of Clinical Microbiology 1999, 37: 558-564. PMID: 9986812, PMCID: PMC84468, DOI: 10.1128/jcm.37.3.558-564.1999.Peer-Reviewed Original ResearchConceptsHGE agentSerum samplesNational referral centerE. chaffeensis antigenSpecific etiologic agentState health departmentsE. chaffeensisHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisHuman monocytic ehrlichiosisReferral centerSerologic testingEhrlichial antigenAverage ageHealth departmentsHuman ehrlichiosisPatientsEtiologic agentProbable casesHigh titersIll individualsGranulocytic ehrlichiosisDisease controlIndirect immunofluorescenceMonocytic ehrlichiosisDiagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum
Comer J, Nicholson W, Sumner J, Olson J, Childs J. Diagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum. Journal Of Clinical Microbiology 1999, 37: 31-34. PMID: 9854059, PMCID: PMC84159, DOI: 10.1128/jcm.37.1.31-34.1999.Peer-Reviewed Original ResearchConceptsDetermination of etiologyAcute-phase seraHuman granulocytic ehrlichiosisSerologic testingHuman ehrlichiosisAcute-phase serum samplesEarly detectionWhole blood specimensPCR assaysBlood specimensPCR testingEtiologic agentGranulocytic ehrlichiosisEtiologyHGE agentSimilar titersSerum samplesExposure historyTitersEhrlichia chaffeensisEhrlichiosisFourfold differenceE. chaffeensisSerumAntibodies
1997
Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis
Paddock C, Sumner J, Shore G, Bartley D, Elie R, McQuade J, Martin C, Goldsmith C, Childs J. Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 2496-2502. PMID: 9316896, PMCID: PMC229999, DOI: 10.1128/jcm.35.10.2496-2502.1997.Peer-Reviewed Original ResearchConceptsE. chaffeensisDH82 cellsEhrlichia chaffeensisArkansas isolateGroESL heat shock operonFatal ehrlichiosisMechanisms of diseasePulmonary insufficiencyClinical presentationMarked thrombocytopeniaSevere diseaseEhrlichial infectionCharacteristic manifestationsPatientsDays postinoculationMolecular evaluationChaffeensisPrimary isolationRRNA gene sequencesDiseaseIsolatesSpecific associationNew isolatesGene sequencesProtein gene
1994
Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis
Dawson J, Stallknecht D, Howerth E, Warner C, Biggie K, Davidson W, Lockhart J, Nettles V, Olson J, Childs J. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Journal Of Clinical Microbiology 1994, 32: 2725-2728. PMID: 7852563, PMCID: PMC264150, DOI: 10.1128/jcm.32.11.2725-2728.1994.Peer-Reviewed Original ResearchConceptsHuman ehrlichiosisE. canisE. chaffeensisEtiologic agentAbsence of seroconversionEhrlichia chaffeensisMacrophage-type cellsE. chaffeensis infectionLymph nodesPeripheral bloodClinical diseaseDay 10InfectionEhrlichia canisEhrlichiosisAntibodiesWhite-tailed deerChaffeensisNegative controlCanisReservoir hostsCompetent reservoirsWeeksTick vectorSeroconversion