2009
Regulation of Aryl Hydrocarbon Receptor Function by Selective Estrogen Receptor Modulators
DuSell CD, Nelson ER, Wittmann BM, Fretz JA, Kazmin D, Thomas RS, Pike JW, McDonnell DP. Regulation of Aryl Hydrocarbon Receptor Function by Selective Estrogen Receptor Modulators. Endocrinology 2009, 24: 33-46. PMID: 19901195, PMCID: PMC2802893, DOI: 10.1210/me.2009-0339.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAryl Hydrocarbon Receptor Nuclear TranslocatorBreast NeoplasmsCell DifferentiationCell LineCell Line, TumorChromatin ImmunoprecipitationDose-Response Relationship, DrugFemaleGene Expression ProfilingGene Expression RegulationHumansMaleMiceOsteoclastsReceptors, Aryl HydrocarbonReceptors, EstrogenRecombinant ProteinsSelective Estrogen Receptor ModulatorsTamoxifenConceptsSelective estrogen receptor modulatorsAryl hydrocarbon receptorEstrogen receptor modulatorsEstrogen receptorReceptor modulatorsBreast cancerAbsence of ERER-independent mannerAryl hydrocarbon receptor functionAgonist/antagonist activityActivity of drugsAhR target genesEstradiol metabolismPharmacological actionsOsteoclast differentiationTamoxifenTherapeutic efficacyActive metaboliteReceptor functionAntagonist activityHydrocarbon receptorCalcium signalingCellular proliferationPotential roleCellular model
2007
Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase
Fretz JA, Shevde NK, Singh S, Darnay BG, Pike JW. Receptor Activator of Nuclear Factor-κB Ligand-Induced Nuclear Factor of Activated T Cells (C1) Autoregulates Its Own Expression in Osteoclasts and Mediates the Up-Regulation of Tartrate-Resistant Acid Phosphatase. Endocrinology 2007, 22: 737-750. PMID: 18063694, PMCID: PMC2262172, DOI: 10.1210/me.2007-0333.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone and BonesCell LineChromatin ImmunoprecipitationHomeostasisIsoenzymesMiceMice, Inbred C57BLNFATC Transcription FactorsOsteoclastsPromoter Regions, GeneticRANK LigandReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTartrate-Resistant Acid PhosphataseTranscription, GeneticUp-RegulationConceptsNFAT membersRNA polymerase IIDNA-binding proteinsSpecific transcription factorsChromatin immunoprecipitation analysisSignal transduction pathwaysAdditional molecular detailsNuclear factorActivated T cells cytoplasmic 1Polymerase IIAcp5 promotersTranscription factorsTransduction pathwaysMolecular detailsTarget genesOwn expressionImmunoprecipitation analysisP1 promoterBone-resorbing cellsReceptor activatorHematopoietic precursorsGenesNuclear factor-κB ligandCytoplasmic 1Time-dependent accumulationMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay