2014
Strain‐Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO‐1 Organization in a Caspase 3‐ and 9‐Dependent Manner
Wu Z, Mirza H, Teo JD, Tan KS. Strain‐Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO‐1 Organization in a Caspase 3‐ and 9‐Dependent Manner. BioMed Research International 2014, 2014: 209163. PMID: 24822183, PMCID: PMC4009109, DOI: 10.1155/2014/209163.Peer-Reviewed Original ResearchConceptsEpithelial barrier compromiseEnterocyte apoptosisHuman colonic epithelial cell line Caco-2Barrier compromiseHuman intestinal epithelial barrier functionIntestinal epithelial barrier functionHuman intestineBlastocystis subtype 7Epithelial cell line Caco-2Caspase-3ST-7Epithelial barrier functionZO-1 organizationStrain-dependent inductionCell line Caco-2Caco-2 monolayersAbdominal painIntestinal symptomsPan-caspase inhibitor Z-VAD-FMKEpithelial permeabilitySubtype 7Subtype 4Epithelial barrierEpithelial resistanceBarrier function
2011
A Metronidazole-Resistant Isolate of Blastocystis spp. Is Susceptible to Nitric Oxide and Downregulates Intestinal Epithelial Inducible Nitric Oxide Synthase by a Novel Parasite Survival Mechanism
Mirza H, Wu Z, Kidwai F, Tan KS. A Metronidazole-Resistant Isolate of Blastocystis spp. Is Susceptible to Nitric Oxide and Downregulates Intestinal Epithelial Inducible Nitric Oxide Synthase by a Novel Parasite Survival Mechanism. Infection And Immunity 2011, 79: 5019-5026. PMID: 21930763, PMCID: PMC3232666, DOI: 10.1128/iai.05632-11.Peer-Reviewed Original ResearchConceptsInducible nitric oxide synthaseMetronidazole-resistant isolatesNitric oxide synthaseNitric oxideOxide synthaseBlastocystis infectionEpithelial inducible nitric oxide synthaseST-7Dose-dependent inhibitionHost innate responseHuman intestinal epitheliumHost defense mechanismsIntestinal epithelial cellsLuminal pathogensAbdominal painIntestinal symptomsImmunocompetent patientsST-4Caco-2 modelChronic infectionControversial pathogenesisInnate responseNO productionBlastocystis sppIntestinal epithelium