2001
Autostimulation of the Epstein-Barr Virus BRLF1 Promoter Is Mediated through Consensus Sp1 and Sp3 Binding Sites
Ragoczy T, Miller G. Autostimulation of the Epstein-Barr Virus BRLF1 Promoter Is Mediated through Consensus Sp1 and Sp3 Binding Sites. Journal Of Virology 2001, 75: 5240-5251. PMID: 11333906, PMCID: PMC114930, DOI: 10.1128/jvi.75.11.5240-5251.2001.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesB-LymphocytesCell Line, TransformedDNA-Binding ProteinsGene DeletionGene Expression Regulation, ViralHerpesvirus 4, HumanHeterotrimeric GTP-Binding ProteinsHumansImmediate-Early ProteinsMolecular Sequence DataMutagenesis, Site-DirectedPromoter Regions, GeneticProtein BindingReceptors, Cell SurfaceSp1 Transcription FactorSp3 Transcription FactorTrans-ActivatorsTranscription FactorsViral ProteinsVirus ActivationConceptsSp1/Sp3 siteLytic cycleSp3 transcription factorsBinding of Sp1Transcriptional start siteSite-directed mutagenesisGel shift analysisBRLF1 promoterReporter-based assaysEpstein–Barr virus Rta proteinCellular Sp1Own geneConsensus Sp1Transcriptional activationCellular proteinsTranscription factorsStart siteDNA bindingOwn expressionMutagenesis studiesRta proteinSp1Reporter activityTranscription factor Zif268B cells
1997
The Locus of Epstein–Barr Virus Terminal Repeat Processing Is Bound with Enhanced Affinity by Sp1 and Sp3
Spain T, Sun R, Miller G. The Locus of Epstein–Barr Virus Terminal Repeat Processing Is Bound with Enhanced Affinity by Sp1 and Sp3. Virology 1997, 237: 137-147. PMID: 9344916, DOI: 10.1006/viro.1997.8770.Peer-Reviewed Original ResearchConceptsRecombination eventsRepeat binding proteinMinimal binding siteAntibody supershift assaysRepeat processingSp1 sitesCellular proteinsLarge internal repeatRecombinogenic regionsInternal repeatsSp1Supershift assaysRecombinant proteinsTerminal repeatBinding proteinLytic cycle inductionEBV lytic cycle inductionCycle inductionProteinAffinity of bindingBinding sitesRepeatsSite 1DNASp3