2018
Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response
Serebrenik YV, Hellerschmied D, Toure M, López-Giráldez F, Brookner D, Crews CM. Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response. Molecular Biology Of The Cell 2018, 29: 1284-1298. PMID: 29851555, PMCID: PMC5994893, DOI: 10.1091/mbc.e17-11-0693.Peer-Reviewed Original ResearchConceptsStress responseTranscriptional stress responseChemical biology strategyStress response mechanismsGolgi structural integrityRNA sequence profilesUncharacterized genesEssential organellesEukaryotic cellsCellular homeostasisMisfolded proteinsGolgi targetingTranscriptional responseProtein substratesOrganelle structureProtein modificationHomeostasis mechanismsGolgi apparatusSecretory systemTargeted proteinsProtein unfoldingFurther revealsMajor siteProteinPrecise processing
2016
The Epstein-Barr Virus Immunoevasins BCRF1 and BPLF1 Are Expressed by a Mechanism Independent of the Canonical Late Pre-initiation Complex
McKenzie J, Lopez-Giraldez F, Delecluse HJ, Walsh A, El-Guindy A. The Epstein-Barr Virus Immunoevasins BCRF1 and BPLF1 Are Expressed by a Mechanism Independent of the Canonical Late Pre-initiation Complex. PLOS Pathogens 2016, 12: e1006008. PMID: 27855219, PMCID: PMC5113994, DOI: 10.1371/journal.ppat.1006008.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternChromatin ImmunoprecipitationEpstein-Barr Virus InfectionsGene Expression Regulation, ViralGene Knockdown TechniquesHEK293 CellsHerpesvirus 4, HumanHigh-Throughput Nucleotide SequencingHumansImmune EvasionReal-Time Polymerase Chain ReactionViral ProteinsViral Regulatory and Accessory ProteinsViral Structural ProteinsConceptsPre-initiation complexViral pre-initiation complexEpstein-Barr virusLate genesViral gene expressionGene regulatorsViral structural proteinsGene expressionStructural proteinsImmunomodulatory proteinsHost immune surveillanceAntiviral immune responseChIP-seq analysisProtein kinase BGLF4Presence of siRNAsEB virusPrimary infectionImmune surveillanceImmune responseAntiviral drugsImmune evasionGene productsViral pathogenesisBCRF1Gamma herpesvirus
2014
A Locus Encompassing the Epstein-Barr Virus bglf4 Kinase Regulates Expression of Genes Encoding Viral Structural Proteins
El-Guindy A, Lopez-Giraldez F, Delecluse HJ, McKenzie J, Miller G. A Locus Encompassing the Epstein-Barr Virus bglf4 Kinase Regulates Expression of Genes Encoding Viral Structural Proteins. PLOS Pathogens 2014, 10: e1004307. PMID: 25166506, PMCID: PMC4148442, DOI: 10.1371/journal.ppat.1004307.Peer-Reviewed Original ResearchConceptsLate gene expressionViral DNA replicationLate genesDNA replicationGene expressionOnly protein kinaseEBV transcriptomeDNA tumor virusesPost-replication eventsViral structural proteinsViral structural componentsWild-type Epstein-Barr virusProtein kinaseEctopic expressionKinase geneControl expressionTranscript levelsKinase activityMRNA-seqBGLF4Structural proteinsRegulate expressionGenesControl lociTumor virus