2014
Synergistic Regulation of Glutamatergic Transmission by Serotonin and Norepinephrine Reuptake Inhibitors in Prefrontal Cortical Neurons*
Yuen EY, Qin L, Wei J, Liu W, Liu A, Yan Z. Synergistic Regulation of Glutamatergic Transmission by Serotonin and Norepinephrine Reuptake Inhibitors in Prefrontal Cortical Neurons*. Journal Of Biological Chemistry 2014, 289: 25177-25185. PMID: 25056951, PMCID: PMC4155681, DOI: 10.1074/jbc.m114.567610.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic Uptake InhibitorsAnimalsBicucullineBlotting, WesternCarrier ProteinsCells, CulturedDesipramineDrug SynergismDynaminsEndocytosisExcitatory Postsynaptic PotentialsFluoxetineP38 Mitogen-Activated Protein KinasesPatch-Clamp TechniquesPrefrontal CortexPyramidal CellsRab5 GTP-Binding ProteinsRatsReceptor, Serotonin, 5-HT1AReceptors, Adrenergic, alpha-2Receptors, AMPASelective Serotonin Reuptake InhibitorsSignal TransductionSynaptic TransmissionConceptsNorepinephrine reuptake inhibitorsExcitatory postsynaptic currentsΑ2-adrenergic receptorsReuptake inhibitorsGlutamatergic transmissionPostsynaptic currentsCortical neuronsAMPAR-mediated excitatory postsynaptic currentsG proteinsPrefrontal cortex pyramidal neuronsDynamin-mediated endocytosisSelective reuptake inhibitorsProtein α subunitsCortex pyramidal neuronsPrefrontal cortical neuronsP38 kinase activityAMPAR surface expressionGood therapeutic effectG-protein modulatorsPyramidal neuronsClinical studiesTherapeutic effectMonoamine systemsAMPA receptorsKinase activityMemory Enhancement by Targeting Cdk5 Regulation of NR2B
Plattner F, Hernández A, Kistler TM, Pozo K, Zhong P, Yuen EY, Tan C, Hawasli AH, Cooke SF, Nishi A, Guo A, Wiederhold T, Yan Z, Bibb JA. Memory Enhancement by Targeting Cdk5 Regulation of NR2B. Neuron 2014, 81: 1070-1083. PMID: 24607229, PMCID: PMC4010123, DOI: 10.1016/j.neuron.2014.01.022.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCells, CulturedCyclin-Dependent Kinase 5FemaleHippocampusMaleMemoryMemory DisordersMiceMice, Inbred C57BLMice, KnockoutMolecular Sequence DataNeuronal PlasticityNeuronsOrgan Culture TechniquesPhosphorylationRatsRats, Sprague-DawleyReceptors, N-Methyl-D-AspartateSynaptic TransmissionConceptsCell surface expressionReceptor cell surface expressionCyclin-dependent kinase 5Cdk5 regulationN-methyl-D-aspartate receptorsRegulatory mechanismsKinase 5NR2B functionSurface expressionNMDAR functionSubunit NR2BSynaptic plasticityEnhancerFundamental roleRegulationMemory formationNMDAR subunit NR2BCognitive enhancersValid treatment strategyPrime targetSynaptic transmissionNR2B phosphorylationNR2BPhosphorylationSurface level
2011
Cellular Mechanisms for Dopamine D4 Receptor-induced Homeostatic Regulation of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors*
Yuen EY, Yan Z. Cellular Mechanisms for Dopamine D4 Receptor-induced Homeostatic Regulation of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors*. Journal Of Biological Chemistry 2011, 286: 24957-24965. PMID: 21622557, PMCID: PMC3137069, DOI: 10.1074/jbc.m111.221416.Peer-Reviewed Original ResearchConceptsGlutamatergic transmissionPFC neuronsPrefrontal cortexCalcium/calmodulin kinase II activityAMPAR responsesIsoxazolepropionic acid (AMPA) receptorsHomeostatic regulationCalmodulin kinase II activityAttention deficit hyperactivity disorderBi-directional regulationDopamine D4 receptorDeficit hyperactivity disorderAberrant dopaminePyramidal neuronsAMPA receptorsKinase II activitySynaptic currentsSynaptic targetingMental illnessAcid receptorsReceptor functionD4 receptorsCaMKII inhibitionActivity stateDistinct signaling mechanisms
2010
Regulation of AMPA receptor channels and synaptic plasticity by cofilin phosphatase Slingshot in cortical neurons
Yuen EY, Liu W, Kafri T, Van Praag H, Yan Z. Regulation of AMPA receptor channels and synaptic plasticity by cofilin phosphatase Slingshot in cortical neurons. The Journal Of Physiology 2010, 588: 2361-2371. PMID: 20442266, PMCID: PMC2915513, DOI: 10.1113/jphysiol.2009.186353.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCells, CulturedCerebral CortexCofilin 1ElectrophysiologyExcitatory Postsynaptic PotentialsImmunohistochemistryLentivirusMicrofilament ProteinsNeuronal PlasticityNeuronsPhosphoric Monoester HydrolasesRatsReceptors, AMPARNA, Small InterferingRNA, ViralSynapsesSynaptic TransmissionConceptsExcitatory postsynaptic currentsCortical neuronsSynaptic transmissionSynaptic plasticityReceptor-mediated excitatory postsynaptic currentsAMPA receptor-mediated excitatory postsynaptic currentsPhosphatase SlingshotActin dynamicsAMPA receptor channelsFunction-blocking antibodiesSmall RNA interferenceAMPAR EPSCsMajor actin depolymerizing factorPostsynaptic currentsCofilin phosphatase SlingshotGluR-1Synaptic functionActin depolymerizing factorSpine morphologyAMPAR traffickingReceptor channelsActin cytoskeletonRNA interferenceNeuronsCofilin activationMechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory
Yuen EY, Liu W, Karatsoreos IN, Ren Y, Feng J, McEwen BS, Yan Z. Mechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory. Molecular Psychiatry 2010, 16: 156-170. PMID: 20458323, PMCID: PMC3108461, DOI: 10.1038/mp.2010.50.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiophysicsCells, CulturedCorticosteroneDose-Response Relationship, DrugDrug InteractionsElectric StimulationExcitatory Amino Acid AgentsExcitatory Postsynaptic PotentialsGene Expression RegulationGlutamic AcidGreen Fluorescent ProteinsHormone AntagonistsImmediate-Early ProteinsIn Vitro TechniquesLong-Term PotentiationMaleMaze LearningMemory, Short-TermMifepristonePatch-Clamp TechniquesPeptidesPrefrontal CortexProtein Serine-Threonine KinasesPyramidal CellsRab GTP-Binding ProteinsRatsRats, Sprague-DawleyReceptors, GlutamateRNA, Small InterferingStress, PsychologicalSynaptic TransmissionTime FactorsTransfectionConceptsPrefrontal cortexGlutamatergic transmissionAcid receptorsPFC pyramidal neuronsAspartic acid (NMDA) receptorsIsoxazolepropionic acid (AMPA) receptorsInduction of serumCorticosteroid stress hormonesFunction of NMDARsStress-induced enhancementSustained potentiationPyramidal neuronsCorticosterone treatmentSynaptic responsesSynaptic transmissionBehavioral stressorsGlucocorticoid-inducible kinaseGlucocorticoid receptorAcute stressStress hormonesΑ-aminoSurface expressionReceptorsUnderlying mechanismReceptor recyclingDelivery of GABAARs to Synapses Is Mediated by HAP1-KIF5 and Disrupted by Mutant Huntingtin
Twelvetrees A, Yuen E, Arancibia-Carcamo I, MacAskill A, Rostaing P, Lumb M, Humbert S, Triller A, Saudou F, Yan Z, Kittler J. Delivery of GABAARs to Synapses Is Mediated by HAP1-KIF5 and Disrupted by Mutant Huntingtin. Neuron 2010, 65: 53-65. PMID: 20152113, PMCID: PMC2841506, DOI: 10.1016/j.neuron.2009.12.007.Peer-Reviewed Original ResearchConceptsHuntingtin-associated protein 1Huntington's diseaseInhibitory postsynaptic currentsMutant huntingtinInhibitory synaptic currentsBrain excitabilityPostsynaptic currentsSynaptic inhibitionTherapeutic approachesSynaptic currentsDiseaseBrain information processingMolecular targetsSynapsesProtein 1Protein 5Protein huntingtinHuntingtinReceptorsPolyglutamine repeatsInhibitionGABAARsExcitability
2009
Assembly of a β2‐adrenergic receptor—GluR1 signalling complex for localized cAMP signalling
Joiner M, Lisé M, Yuen E, Kam A, Zhang M, Hall D, Malik Z, Qian H, Chen Y, Ulrich J, Burette A, Weinberg R, Law P, El‐Husseini A, Yan Z, Hell J. Assembly of a β2‐adrenergic receptor—GluR1 signalling complex for localized cAMP signalling. The EMBO Journal 2009, 29: 482-495. PMID: 19942860, PMCID: PMC2824466, DOI: 10.1038/emboj.2009.344.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesAnimalsCalcium ChannelsCells, CulturedCerebral CortexCyclic AMP-Dependent Protein KinasesDisks Large Homolog 4 ProteinElectrophysiologyGene Expression RegulationGTP-Binding Protein alpha Subunits, GsHippocampusIntracellular Signaling Peptides and ProteinsMembrane ProteinsNeuronsRatsRats, Sprague-DawleyReceptors, Adrenergic, beta-2Receptors, AMPAConceptsUnknown molecular mechanismsMajor target proteinGluR1 surface expressionLocalized cAMPTarget proteinsMolecular mechanismsPhosphorylation of GluR1Selective regulationAdenylyl cyclaseProteinPSD-95PhosphorylationSurface expressionGlutamate receptor subunits GluR1PKAAssemblyPostsynaptic sitesCentral noradrenergic signalingHomologuesSubunit GluR1ComplexesCAMPSignalingAdrenergic receptorsStargazin
2008
Postsynaptic Density-95 (PSD-95) and Calcineurin Control the Sensitivity of N-Methyl-d-aspartate Receptors to Calpain Cleavage in Cortical Neurons
Yuen EY, Ren Y, Yan Z. Postsynaptic Density-95 (PSD-95) and Calcineurin Control the Sensitivity of N-Methyl-d-aspartate Receptors to Calpain Cleavage in Cortical Neurons. Molecular Pharmacology 2008, 74: 360-370. PMID: 18445709, PMCID: PMC2858625, DOI: 10.1124/mol.108.046813.Peer-Reviewed Original ResearchConceptsN-methyl-D-aspartate receptorsCortical neuronsNR2B subunitExtrasynaptic N-methyl-D-aspartate receptorsPermeable glutamate receptorsProtein postsynaptic densityTransient forebrain ischemiaCultured cortical neuronsDependent protease calpainSynaptic NMDAR responsesPostsynaptic density-95Calpain-dependent mannerWhole-cell currentsExcitotoxic conditionsForebrain ischemiaCortical slicesNMDA treatmentNMDAR currentsNMDAR responsesNMDAR functionSubstrates of calpainGlutamate receptorsNeuronal excitabilityCalpain cleavageDensity-95Activation of 5-HT2A/C Receptors Counteracts 5-HT1A Regulation of N-Methyl-D-aspartate Receptor Channels in Pyramidal Neurons of Prefrontal Cortex*
Yuen EY, Jiang Q, Chen P, Feng J, Yan Z. Activation of 5-HT2A/C Receptors Counteracts 5-HT1A Regulation of N-Methyl-D-aspartate Receptor Channels in Pyramidal Neurons of Prefrontal Cortex*. Journal Of Biological Chemistry 2008, 283: 17194-17204. PMID: 18442977, PMCID: PMC2427346, DOI: 10.1074/jbc.m801713200.Peer-Reviewed Original ResearchConceptsPFC pyramidal neuronsExtracellular signal-regulated kinasePyramidal neuronsPrefrontal cortexNMDAR currentsN-methyl-D-aspartate receptor currentsN-methyl-D-aspartate receptor channelsSynaptic NMDAR responsesNMDAR responsesNMDAR functionActivation of ERKDendritic processesReceptor currentsPFC neuronsSignal-regulated kinaseReceptor activationMental disordersImmunocytochemical studyIntact animalsCounteractive effectsReceptor channelsInhibitory effectNeuronsCellular knockdownDependent pathway
2007
The Phosphorylation State of GluR1 Subunits Determines the Susceptibility of AMPA Receptors to Calpain Cleavage*
Yuen EY, Liu W, Yan Z. The Phosphorylation State of GluR1 Subunits Determines the Susceptibility of AMPA Receptors to Calpain Cleavage*. Journal Of Biological Chemistry 2007, 282: 16434-16440. PMID: 17428797, DOI: 10.1074/jbc.m701283200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCalpainCells, CulturedCerebral CortexEnzyme ActivationNeuronsPhosphoprotein PhosphatasesPhosphorylationProtein Phosphatase 1Protein Processing, Post-TranslationalProtein SubunitsRatsRats, Sprague-DawleyReceptors, AMPATime FactorsConceptsCalpain cleavagePhosphorylation stateProteolytic cleavageDependent protein kinase IITerminal fusion proteinEffect of phosphorylationProtein phosphatase 1/2AProtein kinase IIPhosphorylation sitesProtein kinaseCalpain cleavage sitesGluR1 subunitKinase IIFusion proteinActive CaMKIIAMPAR currentsCalpain regulationCleavage siteIsoxazoleproprionic acid (AMPA) receptorSubunitsIonotropic glutamate receptorsN-methyl-D-aspartate receptorsPhysiological studiesExcitatory synaptic transmissionAMPA receptor currentsCalpain regulation of AMPA receptor channels in cortical pyramidal neurons
Yuen EY, Gu Z, Yan Z. Calpain regulation of AMPA receptor channels in cortical pyramidal neurons. The Journal Of Physiology 2007, 580: 241-254. PMID: 17234699, PMCID: PMC2075435, DOI: 10.1113/jphysiol.2006.122754.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCalpainCells, CulturedCerebral CortexElectrophysiologyEndocytosisGerbillinaeImmunohistochemistryMaleMAP Kinase Kinase 2NeuronsNeuroprotective AgentsPatch-Clamp TechniquesPyramidal CellsRatsRats, Sprague-DawleyReceptors, AMPAReceptors, Cell SurfaceReceptors, N-Methyl-D-AspartateStimulation, ChemicalSynapsesSynaptic TransmissionConceptsCortical pyramidal neuronsNMDA receptorsPyramidal neuronsAMPA receptorsNMDA treatmentCalpain inhibitorsIschaemia-induced depressionFast excitatory synaptic transmissionTransient ischemic insultAMPAR-mediated currentsExcitatory synaptic transmissionCalpain-dependent mannerAMPA receptor channelsSelective calpain inhibitorsExcitotoxic conditionsIschemic animalsIschemic insultNeuronal excitotoxicityAMPAR currentsAMPA responsesCortical culturesExtracellular glutamateGlutamate receptorsGlutamate triggersNeuronal excitability
2005
Activation of Dopamine D4 Receptors Induces Synaptic Translocation of Ca2+/Calmodulin-Dependent Protein Kinase II in Cultured Prefrontal Cortical Neurons
Gu Z, Jiang Q, Yuen E, Yan Z. Activation of Dopamine D4 Receptors Induces Synaptic Translocation of Ca2+/Calmodulin-Dependent Protein Kinase II in Cultured Prefrontal Cortical Neurons. Molecular Pharmacology 2005, 69: 813-822. PMID: 16365279, DOI: 10.1124/mol.105.018853.Peer-Reviewed Original ResearchConceptsD4 receptor activationDependent protein kinase IIProtein kinase IIAlpha-CaMKIID4 receptorsDopamine D4 receptorCaMKII translocationKinase IIReceptor activationF-actinSynaptic translocationPostsynaptic sitesBinding of CaMKIITrisphosphate receptor/Ca2Receptor-mediated excitatory postsynaptic currentsAMPA receptor-mediated excitatory postsynaptic currentsPhospholipase C/inositolPrefrontal cortexCalmodulin binding siteExcitatory postsynaptic currentsGlutamate receptor 1 (GluR1) subunitPrefrontal cortical neuronsD4 receptor stimulationAutophosphorylation sitesSubcellular localization