2014
Synergistic Regulation of Glutamatergic Transmission by Serotonin and Norepinephrine Reuptake Inhibitors in Prefrontal Cortical Neurons*
Yuen EY, Qin L, Wei J, Liu W, Liu A, Yan Z. Synergistic Regulation of Glutamatergic Transmission by Serotonin and Norepinephrine Reuptake Inhibitors in Prefrontal Cortical Neurons*. Journal Of Biological Chemistry 2014, 289: 25177-25185. PMID: 25056951, PMCID: PMC4155681, DOI: 10.1074/jbc.m114.567610.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic Uptake InhibitorsAnimalsBicucullineBlotting, WesternCarrier ProteinsCells, CulturedDesipramineDrug SynergismDynaminsEndocytosisExcitatory Postsynaptic PotentialsFluoxetineP38 Mitogen-Activated Protein KinasesPatch-Clamp TechniquesPrefrontal CortexPyramidal CellsRab5 GTP-Binding ProteinsRatsReceptor, Serotonin, 5-HT1AReceptors, Adrenergic, alpha-2Receptors, AMPASelective Serotonin Reuptake InhibitorsSignal TransductionSynaptic TransmissionConceptsNorepinephrine reuptake inhibitorsExcitatory postsynaptic currentsΑ2-adrenergic receptorsReuptake inhibitorsGlutamatergic transmissionPostsynaptic currentsCortical neuronsAMPAR-mediated excitatory postsynaptic currentsG proteinsPrefrontal cortex pyramidal neuronsDynamin-mediated endocytosisSelective reuptake inhibitorsProtein α subunitsCortex pyramidal neuronsPrefrontal cortical neuronsP38 kinase activityAMPAR surface expressionGood therapeutic effectG-protein modulatorsPyramidal neuronsClinical studiesTherapeutic effectMonoamine systemsAMPA receptorsKinase activity
2010
Mechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory
Yuen EY, Liu W, Karatsoreos IN, Ren Y, Feng J, McEwen BS, Yan Z. Mechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory. Molecular Psychiatry 2010, 16: 156-170. PMID: 20458323, PMCID: PMC3108461, DOI: 10.1038/mp.2010.50.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiophysicsCells, CulturedCorticosteroneDose-Response Relationship, DrugDrug InteractionsElectric StimulationExcitatory Amino Acid AgentsExcitatory Postsynaptic PotentialsGene Expression RegulationGlutamic AcidGreen Fluorescent ProteinsHormone AntagonistsImmediate-Early ProteinsIn Vitro TechniquesLong-Term PotentiationMaleMaze LearningMemory, Short-TermMifepristonePatch-Clamp TechniquesPeptidesPrefrontal CortexProtein Serine-Threonine KinasesPyramidal CellsRab GTP-Binding ProteinsRatsRats, Sprague-DawleyReceptors, GlutamateRNA, Small InterferingStress, PsychologicalSynaptic TransmissionTime FactorsTransfectionConceptsPrefrontal cortexGlutamatergic transmissionAcid receptorsPFC pyramidal neuronsAspartic acid (NMDA) receptorsIsoxazolepropionic acid (AMPA) receptorsInduction of serumCorticosteroid stress hormonesFunction of NMDARsStress-induced enhancementSustained potentiationPyramidal neuronsCorticosterone treatmentSynaptic responsesSynaptic transmissionBehavioral stressorsGlucocorticoid-inducible kinaseGlucocorticoid receptorAcute stressStress hormonesΑ-aminoSurface expressionReceptorsUnderlying mechanismReceptor recyclingDelivery of GABAARs to Synapses Is Mediated by HAP1-KIF5 and Disrupted by Mutant Huntingtin
Twelvetrees A, Yuen E, Arancibia-Carcamo I, MacAskill A, Rostaing P, Lumb M, Humbert S, Triller A, Saudou F, Yan Z, Kittler J. Delivery of GABAARs to Synapses Is Mediated by HAP1-KIF5 and Disrupted by Mutant Huntingtin. Neuron 2010, 65: 53-65. PMID: 20152113, PMCID: PMC2841506, DOI: 10.1016/j.neuron.2009.12.007.Peer-Reviewed Original ResearchConceptsHuntingtin-associated protein 1Huntington's diseaseInhibitory postsynaptic currentsMutant huntingtinInhibitory synaptic currentsBrain excitabilityPostsynaptic currentsSynaptic inhibitionTherapeutic approachesSynaptic currentsDiseaseBrain information processingMolecular targetsSynapsesProtein 1Protein 5Protein huntingtinHuntingtinReceptorsPolyglutamine repeatsInhibitionGABAARsExcitability
2009
Dopamine D4 Receptors Regulate AMPA Receptor Trafficking and Glutamatergic Transmission in GABAergic Interneurons of Prefrontal Cortex
Yuen EY, Yan Z. Dopamine D4 Receptors Regulate AMPA Receptor Trafficking and Glutamatergic Transmission in GABAergic Interneurons of Prefrontal Cortex. Journal Of Neuroscience 2009, 29: 550-562. PMID: 19144855, PMCID: PMC2768380, DOI: 10.1523/jneurosci.5050-08.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsCalcineurinColchicineDopamine AgentsEnzyme InhibitorsGamma-Aminobutyric AcidGlutamate DecarboxylaseGlutamic AcidGreen Fluorescent ProteinsIn Vitro TechniquesInterneuronsMiceMice, TransgenicMicrofilament ProteinsPatch-Clamp TechniquesPhalloidinePhosphoric Monoester HydrolasesPrefrontal CortexProtein TransportRatsReceptors, AMPAReceptors, Dopamine D4RNA, Small InterferingSynaptic TransmissionTransfectionTubulin ModulatorsConceptsGABAergic interneuronsAttention deficit hyperactivity disorderGABAergic inhibitionGlutamatergic transmissionPrefrontal cortexAMPAR-mediated synaptic transmissionLocal circuit neuronsPFC pyramidal neuronsExcitatory synaptic strengthAbnormal neural synchronyAMPA receptor traffickingDopamine D4 receptorAMPAR EPSCsDeficit hyperactivity disorderPFC interneuronsPyramidal neuronsMajor actin depolymerizing factorMemory disturbanceSynaptic transmissionAMPA receptorsCortical circuitsNeuronal activityPersistent suppressionInterneuronsMental disorders
2007
Calpain regulation of AMPA receptor channels in cortical pyramidal neurons
Yuen EY, Gu Z, Yan Z. Calpain regulation of AMPA receptor channels in cortical pyramidal neurons. The Journal Of Physiology 2007, 580: 241-254. PMID: 17234699, PMCID: PMC2075435, DOI: 10.1113/jphysiol.2006.122754.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCalpainCells, CulturedCerebral CortexElectrophysiologyEndocytosisGerbillinaeImmunohistochemistryMaleMAP Kinase Kinase 2NeuronsNeuroprotective AgentsPatch-Clamp TechniquesPyramidal CellsRatsRats, Sprague-DawleyReceptors, AMPAReceptors, Cell SurfaceReceptors, N-Methyl-D-AspartateStimulation, ChemicalSynapsesSynaptic TransmissionConceptsCortical pyramidal neuronsNMDA receptorsPyramidal neuronsAMPA receptorsNMDA treatmentCalpain inhibitorsIschaemia-induced depressionFast excitatory synaptic transmissionTransient ischemic insultAMPAR-mediated currentsExcitatory synaptic transmissionCalpain-dependent mannerAMPA receptor channelsSelective calpain inhibitorsExcitotoxic conditionsIschemic animalsIschemic insultNeuronal excitotoxicityAMPAR currentsAMPA responsesCortical culturesExtracellular glutamateGlutamate receptorsGlutamate triggersNeuronal excitability
2006
Adrenergic modulation of NMDA receptors in prefrontal cortex is differentially regulated by RGS proteins and spinophilin
Liu W, Yuen EY, Allen PB, Feng J, Greengard P, Yan Z. Adrenergic modulation of NMDA receptors in prefrontal cortex is differentially regulated by RGS proteins and spinophilin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 18338-18343. PMID: 17101972, PMCID: PMC1838752, DOI: 10.1073/pnas.0604560103.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic currentsNMDA receptorsAlpha1-ARNMDAR currentsPrefrontal cortexNoradrenergic systemPFC neuronsAlpha2-AR agonistNMDAR EPSC amplitudePFC pyramidal neuronsPaired-pulse ratioAlpha2-adrenergic receptorsPhospholipase C-inositolEffect of RGS4Norepinephrine transporter inhibitorG protein-coupled receptorsSpinophilin knockout miceProtein-coupled receptorsAdrenergic modulationPyramidal neuronsPFC slicesPostsynaptic currentsMood controlMutant miceTransporter inhibitors
2005
Serotonin 5-HT1A Receptors Regulate NMDA Receptor Channels through a Microtubule-Dependent Mechanism
Yuen EY, Jiang Q, Chen P, Gu Z, Feng J, Yan Z. Serotonin 5-HT1A Receptors Regulate NMDA Receptor Channels through a Microtubule-Dependent Mechanism. Journal Of Neuroscience 2005, 25: 5488-5501. PMID: 15944377, PMCID: PMC6724987, DOI: 10.1523/jneurosci.1187-05.2005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesDendritesExtracellular Signal-Regulated MAP KinasesIn Vitro TechniquesKinesinsMembrane PotentialsMicrotubulesMolecular Motor ProteinsNeuronsPatch-Clamp TechniquesPrefrontal CortexProtein SubunitsProtein TransportRatsReceptor, Serotonin, 5-HT1AReceptors, N-Methyl-D-AspartateSerotoninSerotonin 5-HT1 Receptor AgonistsSynapsesConceptsMicrotubule-dependent mechanismMEK/ERKNMDA receptorsPrefrontal cortexMicrotubule stabilityDendritic transportNMDAR interactionMicrotubule assemblyBiochemical evidenceMEK inhibitorsPFC pyramidal neuronsNMDA receptor channelsRole of serotoninCaMKIIERKNeuronal dendritesNMDAR functionMicrotubule stabilizerPathological conditionsPrimary targetReceptor channelsPyramidal neuronsNMDAR currentsSerotonin systemReceptorsRegulation of N-Methyl-D-aspartate Receptors by Calpain in Cortical Neurons*
Wu HY, Yuen EY, Lu YF, Matsushita M, Matsui H, Yan Z, Tomizawa K. Regulation of N-Methyl-D-aspartate Receptors by Calpain in Cortical Neurons*. Journal Of Biological Chemistry 2005, 280: 21588-21593. PMID: 15790561, DOI: 10.1074/jbc.m501603200.Peer-Reviewed Original ResearchConceptsN-methyl-D-aspartate receptorsNMDA receptorsNMDAR currentsCortical neuronsCalpain inhibitorsCultured cortical neuronsCortical pyramidal neuronsDynamin inhibitory peptideFunction of NMDARsInhibitory peptidesInvolvement of calpainNMDAR-mediated currentsNMDAR channel functionSelective calpain inhibitorsNMDAR internalizationNMDAR overstimulationNeuroprotective mechanismsPyramidal neuronsNMDA treatmentBath applicationIntracellular injectionNR2B subunitCalcium entryPathologic functionsProlonged treatment