2024
Durable Mixed Chimerism May Permit Subsequent Immunosuppression-Free Intestinal Transplantation - a Proof-of-Principle Study.
Patwardhan S, Gunes ME, Manell E, Hong J, Jordache P, Chauhan I, Almesallmy A, Mulder H, Ekanayake-Alper D, Hajosi D, Ko HM, Sanmugarajah K, Cetrulo CL, Nowak G, Sachs DH, Sykes M, Weiner J. Durable Mixed Chimerism May Permit Subsequent Immunosuppression-Free Intestinal Transplantation - a Proof-of-Principle Study. Am J Transplant 2024 PMID: 39442670, DOI: 10.1016/j.ajt.2024.10.014.Peer-Reviewed Original ResearchDevelopment of a large animal orthotopic intestinal transplantation model with long-term survival for study of immunologic outcomes
1. Merl, S., Chen, B., Gunes, M. E., Atta, H., Yang, K., Ekanayake-Alper, D., Hajosi, D., Huang, F., Bhola, B., Patwardhan, S., Jordache, P., Nowak, G., Kato, T., Sykes, M., Yamada, K., & Weiner, J. (2024). Development of a large animal orthotopic intestinal transplantation model with long-term survival for study of immunologic outcomes. Frontiers in Transplantation, Section Transplantation Immunology, 3, 1367486Peer-Reviewed Original ResearchSedation Efficacy of Midazolam in Conjunction with Ketamine and Alfaxalone in Female Laboratory Guinea Pigs (Cavia porcellus).
Wharton KN, Walsh CA, Haulter M, Ekanayake D, Ekanayake-Alper D. Sedation Efficacy of Midazolam in Conjunction with Ketamine and Alfaxalone in Female Laboratory Guinea Pigs (Cavia porcellus). J Am Assoc Lab Anim Sci 2024 PMID: 39074958, DOI: 10.30802/AALAS-JAALAS-24-000028.Peer-Reviewed Original ResearchDevelopment of a large animal orthotopic intestinal transplantation model with long-term survival for study of immunologic outcomes.
Merl S, Chen B, Gunes ME, Atta H, Yang K, Ekanayake-Alper D, Hajosi D, Huang F, Bhola B, Patwardhan S, Jordache P, Nowak G, Martinez M, Kato T, Sykes M, Yamada K, Weiner J. Development of a large animal orthotopic intestinal transplantation model with long-term survival for study of immunologic outcomes. Front Transplant 2024, 3: 1367486. PMID: 38993771, DOI: 10.3389/frtra.2024.1367486.Peer-Reviewed Original ResearchA Comparison of Three Anesthetic Drug Combinations for Use in Inducing Surgical Anesthesia in Female Guinea Pigs (Cavia porcellus).
Avelino JA, Walsh CA, Wharton KN, Ekanayake D, Ekanayake-Alper D. A Comparison of Three Anesthetic Drug Combinations for Use in Inducing Surgical Anesthesia in Female Guinea Pigs (Cavia porcellus). J Am Assoc Lab Anim Sci 2024, 63: 182-189. PMID: 38182132, DOI: 10.30802/AALAS-JAALAS-23-000064.Peer-Reviewed Original Research
2023
Chronic GCPII (glutamate‐carboxypeptidase‐II) inhibition reduces pT217Tau levels in the entorhinal and dorsolateral prefrontal cortices of aged macaques
Bathla S, Datta D, Liang F, Barthelemy N, Wiseman R, Slusher B, Asher J, Zeiss C, Ekanayake‐Alper D, Holden D, Terwilliger G, Duque A, Arellano J, van Dyck C, Bateman R, Xie Z, Nairn A, Arnsten A. Chronic GCPII (glutamate‐carboxypeptidase‐II) inhibition reduces pT217Tau levels in the entorhinal and dorsolateral prefrontal cortices of aged macaques. Alzheimer's & Dementia: Translational Research & Clinical Interventions 2023, 9: e12431. PMID: 37915375, PMCID: PMC10617575, DOI: 10.1002/trc2.12431.Peer-Reviewed Original ResearchSporadic Alzheimer's diseaseEntorhinal cortexGCPII inhibitionDorsolateral prefrontal cortexChronic inhibitionTau pathologyTau hyperphosphorylationAged macaquesType 3 metabotropic glutamate receptorAlzheimer's diseasePrefrontal cortexRhesus macaquesVehicle-treated monkeysAged rhesus macaquesMetabotropic glutamate receptorsApparent side effectsAmyloid beta 1Regulation of calciumGCPII inhibitorsKey etiological factorGCPII activityPrimate dlPFCNeuronal damageCSF analysisCalcium dysregulation
2021
Expression of human CD47 in pig glomeruli prevents proteinuria and prolongs graft survival following pig-to-baboon xenotransplantation.
Takeuchi K, Ariyoshi Y, Shimizu A, Okumura Y, Cara-Fuentes G, Garcia GE, Pomposelli T, Watanabe H, Boyd L, Ekanayake-Alper DK, Amarnath D, Sykes M, Sachs DH, Johnson RJ, Yamada K. Expression of human CD47 in pig glomeruli prevents proteinuria and prolongs graft survival following pig-to-baboon xenotransplantation. Xenotransplantation 2021, 28: e12708. PMID: 34418164, DOI: 10.1111/xen.12708.Peer-Reviewed Original ResearchAntibody reactivity with new antigens revealed in multi-transgenic triple knockout pigs may cause early loss of pig kidneys in baboons.
Ariyoshi Y, Takeuchi K, Pomposelli T, Ekanayake-Alper DK, Shimizu A, Boyd L, Estime E, Ohta M, Asfour A, Scott Arn J, Ayares D, Lorber M, Sykes M, Sachs D, Yamada K. Antibody reactivity with new antigens revealed in multi-transgenic triple knockout pigs may cause early loss of pig kidneys in baboons. Xenotransplantation 2021, 28: e12642. PMID: 32909301, DOI: 10.1111/xen.12642.Peer-Reviewed Original Research
2020
Impact of CMV Reactivation, Treatment Approaches, and Immune Reconstitution in a Nonmyeloablative Tolerance Induction Protocol in Cynomolgus Macaques.
Alonso-Guallart P, Duran-Struuck R, Zitsman JS, Sameroff S, Pereira M, Stern J, Berglund E, Llore N, Pierre G, Lopes E, Kofman SB, Danton M, Sondermeijer HP, Woodland D, Kato Y, Ekanayake-Alper DK, Iuga AC, Wuu CS, Wu A, Lipkin WI, Tokarz R, Sykes M, Griesemer A. Impact of CMV Reactivation, Treatment Approaches, and Immune Reconstitution in a Nonmyeloablative Tolerance Induction Protocol in Cynomolgus Macaques. Transplantation 2020, 104: 270-279. PMID: 31385931, DOI: 10.1097/TP.0000000000002893.Peer-Reviewed Original ResearchSafety and pharmacodynamics of anti-CD2 monoclonal antibody treatment in cynomolgus macaques - an experimental study.
Berglund E, Alonso-Guallart P, Danton M, Sellberg F, Binder C, Fröbom R, Berglund D, Llore N, Sakai H, Iuga A, Ekanayake-Alper D, Reimann KA, Sachs DH, Sykes M, Griesemer A. Safety and pharmacodynamics of anti-CD2 monoclonal antibody treatment in cynomolgus macaques - an experimental study. Transpl Int 2020, 33: 98-107. PMID: 31523849, DOI: 10.1111/tri.13524.Peer-Reviewed Original ResearchIntra-bone bone marrow transplantation from hCD47 transgenic pigs to baboons prolongs chimerism to >60 days and promotes increased porcine lung transplant survival.
Watanabe H, Ariyoshi Y, Pomposelli T, Takeuchi K, Ekanayake-Alper DK, Boyd LK, Arn SJ, Sahara H, Shimizu A, Ayares D, Lorber MI, Sykes M, Sachs DH, Yamada K. Intra-bone bone marrow transplantation from hCD47 transgenic pigs to baboons prolongs chimerism to >60 days and promotes increased porcine lung transplant survival. Xenotransplantation 2020, 27: e12552. PMID: 31544995, DOI: 10.1111/xen.12552.Peer-Reviewed Original Research
2019
Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions
Pearson JA, Tai N, Ekanayake-Alper DK, Peng J, Hu Y, Hager K, Compton S, Wong FS, Smith PC, Wen L. Norovirus Changes Susceptibility to Type 1 Diabetes by Altering Intestinal Microbiota and Immune Cell Functions. Frontiers In Immunology 2019, 10: 2654. PMID: 31798584, PMCID: PMC6863139, DOI: 10.3389/fimmu.2019.02654.Peer-Reviewed Original ResearchConceptsExpansion of TregsNOD miceT cellsMNV4 infectionMucosal immunityNon-obese diabetic (NOD) mouse modelGerm-free NOD miceFirmicutes/Bacteroidetes ratioProinflammatory T cellsRole of norovirusesTuft cell markersDevelopment of T1DInflammatory T cellsCommon enteric virusesB cell subsetsDiabetic mouse modelImmune cell functionType 1 diabetes susceptibilityEnteric virusesNaïve splenocytesT1D protectionTreg numbersImmunological changesMucosal antibodiesT1D development
2018
GalT-KO pig lungs are highly susceptible to acute vascular rejection in baboons, which may be mitigated by transgenic expression of hCD47 on porcine blood vessels.
Watanabe H, Sahara H, Nomura S, Tanabe T, Ekanayake-Alper DK, Boyd LK, Louras NJ, Asfour A, Danton MA, Ho SH, Arn SJ, Hawley RJ, Shimizu A, Nagayasu T, Ayares D, Lorber MI, Sykes M, Sachs DH, Yamada K. GalT-KO pig lungs are highly susceptible to acute vascular rejection in baboons, which may be mitigated by transgenic expression of hCD47 on porcine blood vessels. Xenotransplantation 2018, 25: e12391. PMID: 29527745, DOI: 10.1111/xen.12391.Peer-Reviewed Original ResearchRetrospective Review of Surgical Outcomes and Pair-housing Success in Vasectomized Rhesus Macaques (Macaca mulatta).
Ekanayake-Alper DK, Wilson SR, Scholz JA. Retrospective Review of Surgical Outcomes and Pair-housing Success in Vasectomized Rhesus Macaques (Macaca mulatta). Comparative Medicine 2018, 68: 168-176. PMID: 29663943, PMCID: PMC5897974.Peer-Reviewed Original ResearchConceptsAdult male macaquesPostoperative complicationsRetrospective reviewSurgical outcomesRhesus macaquesMale macaquesSurgical site infectionFemale partnersAdult rhesus macaquesAdult male rhesus macaquesMale rhesus macaquesIncisional swellingAnalgesic therapyComplication rateNSAID treatmentSite infectionBenign complicationComplicationsInvasive methodSperm granulomaUnwanted breeding
2017
Role of Intrinsic (Graft) Versus Extrinsic (Host) Factors in the Growth of Transplanted Organs Following Allogeneic and Xenogeneic Transplantation.
Tanabe T, Watanabe H, Shah JA, Sahara H, Shimizu A, Nomura S, Asfour A, Danton M, Boyd L, Dardenne Meyers A, Ekanayake-Alper DK, Sachs DH, Yamada K. Role of Intrinsic (Graft) Versus Extrinsic (Host) Factors in the Growth of Transplanted Organs Following Allogeneic and Xenogeneic Transplantation. Am J Transplant 2017, 17: 1778-1790. PMID: 28117931, DOI: 10.1111/ajt.14210.Peer-Reviewed Original Research
2012
JBP1 and JBP2 proteins are Fe2+/2-oxoglutarate-dependent dioxygenases regulating hydroxylation of thymidine residues in trypanosome DNA.
Cliffe LJ, Hirsch G, Wang J, Ekanayake D, Bullard W, Hu M, Wang Y, Sabatini R. JBP1 and JBP2 proteins are Fe2+/2-oxoglutarate-dependent dioxygenases regulating hydroxylation of thymidine residues in trypanosome DNA. J Biol Chem 2012, 287: 19886-95. PMID: 22514282, DOI: 10.1074/jbc.M112.341974.Peer-Reviewed Original Research
2011
Epigenetic regulation of polymerase II transcription initiation in Trypanosoma cruzi: modulation of nucleosome abundance, histone modification, and polymerase occupancy by O-linked thymine DNA glucosylation.
Ekanayake D, Sabatini R. Epigenetic regulation of polymerase II transcription initiation in Trypanosoma cruzi: modulation of nucleosome abundance, histone modification, and polymerase occupancy by O-linked thymine DNA glucosylation. Eukaryot Cell 2011, 10: 1465-72. PMID: 21926332, DOI: 10.1128/EC.05185-11.Peer-Reviewed Original ResearchEpigenetic regulation of transcription and virulence in Trypanosoma cruzi by O-linked thymine glucosylation of DNA.
Ekanayake DK, Minning T, Weatherly B, Gunasekera K, Nilsson D, Tarleton R, Ochsenreiter T, Sabatini R. Epigenetic regulation of transcription and virulence in Trypanosoma cruzi by O-linked thymine glucosylation of DNA. Mol Cell Biol 2011, 31: 1690-700. PMID: 21321080, DOI: 10.1128/MCB.01277-10.Peer-Reviewed Original Research
2007
JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei.
Kieft R, Brand V, Ekanayake DK, Sweeney K, DiPaolo C, Reznikoff WS, Sabatini R. JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei. Mol Biochem Parasitol 2007, 156: 24-31. PMID: 17706299, DOI: 10.1016/j.molbiopara.2007.06.010.Peer-Reviewed Original ResearchTransmission dynamics of Cryptosporidium infection in a natural population of non-human primates at Polonnaruwa, Sri Lanka.
Ekanayake DK, Welch DM, Kieft R, Hajduk S, Dittus WP. Transmission dynamics of Cryptosporidium infection in a natural population of non-human primates at Polonnaruwa, Sri Lanka. Am J Trop Med Hyg 2007, 77: 818-22. PMID: 17984333.Peer-Reviewed Original Research