2023
FUS Alters circRNA Metabolism in Human Motor Neurons Carrying the ALS-Linked P525L Mutation
Colantoni A, Capauto D, Alfano V, D’Ambra E, D’Uva S, Tartaglia G, Morlando M. FUS Alters circRNA Metabolism in Human Motor Neurons Carrying the ALS-Linked P525L Mutation. International Journal Of Molecular Sciences 2023, 24: 3181. PMID: 36834591, PMCID: PMC9968238, DOI: 10.3390/ijms24043181.Peer-Reviewed Original ResearchConceptsRNA metabolismHuman motor neuronsNuclear/cytoplasmic partitioningNon-canonical splicing eventsAspects of RNASubset of circRNAsCytoplasmic partitioningCytoplasmic circRNAsSplicing eventsMutant proteinsCircular RNAsMotor neuronsAlu repeatsExon compositionCircRNAsProteinRNAMutationsFUS mutationsKey eventsFUSPreferential bindingP525L mutationMetabolismPivotal role
2018
A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons
Capauto D, Colantoni A, Lu L, Santini T, Peruzzi G, Biscarini S, Morlando M, Shneider NA, Caffarelli E, Laneve P, Bozzoni I. A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons. Molecular Neurobiology 2018, 55: 7635-7651. PMID: 29430619, PMCID: PMC6132778, DOI: 10.1007/s12035-018-0884-4.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisMotor neuronsFUS mutationsMouse embryonic stem cellsPathogenesis of ALSMiRNA-dependent regulationCorresponding mRNA targetsNeuron-specific RNATypes of RNAEmbryonic stem cellsCross talkGlutamate α-aminoIsoxazole propionic acidDisease-associated mutationsMolecular cross talkDisturbance of Ca2Juvenile-onset formMiRNA repressionRegulatory circuitryTranscriptome profilingMRNA targetsMultifunctional proteinLong RNAsMN degenerationExcitatory neurotransmission
2017
FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons
Errichelli L, Dini Modigliani S, Laneve P, Colantoni A, Legnini I, Capauto D, Rosa A, De Santis R, Scarfò R, Peruzzi G, Lu L, Caffarelli E, Shneider NA, Morlando M, Bozzoni I. FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons. Nature Communications 2017, 8: 14741. PMID: 28358055, PMCID: PMC5379105, DOI: 10.1038/ncomms14741.Peer-Reviewed Original ResearchConceptsMouse motor neuronsCircRNA biogenesisRNA-binding protein FUSEmbryonic stem cell-derived motor neuronsCircular RNA productionRNA biosynthetic processesCircular RNA expressionStem cell-derived motor neuronsCell-derived motor neuronsAmyotrophic lateral sclerosisMost circRNAsFUS mutantsSplicing junctionsProtein FUSSplicing reactionBiosynthetic processesRNA productionToxic gainCircRNAsMotor neuronsBiogenesisRNA expressionFunction activityNuclear levelsFUS
2015
DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1
Santopolo S, Boccaccini A, Lorrai R, Ruta V, Capauto D, Minutello E, Serino G, Costantino P, Vittorioso P. DOF AFFECTING GERMINATION 2 is a positive regulator of light-mediated seed germination and is repressed by DOF AFFECTING GERMINATION 1. BMC Plant Biology 2015, 15: 72. PMID: 25850831, PMCID: PMC4355143, DOI: 10.1186/s12870-015-0453-1.Peer-Reviewed Original ResearchConceptsSeed germination processPositive regulatorSeed germinationGermination processSeed germination phenotypesChromatin immuno-precipitation analysisImmuno-precipitation analysisGERMINATION 1Mutant seedsGermination phenotypePhytochrome BMaster repressorGibberellin biosynthesisGermination capabilityGermination potentialGerminationRepressorMutantsSeedsOpposite rolesRegulatorEnvironmental factorsDAG2ExpressionRed light
2014
Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination
Boccaccini A, Santopolo S, Capauto D, Lorrai R, Minutello E, Belcram K, Palauqui JC, Costantino P, Vittorioso P. Independent and interactive effects of DOF affecting germination 1 (DAG1) and the Della proteins GA insensitive (GAI) and Repressor of ga1-3(RGA) in embryo development and seed germination. BMC Plant Biology 2014, 14: 200. PMID: 25064446, PMCID: PMC4222566, DOI: 10.1186/s12870-014-0200-z.Peer-Reviewed Original ResearchConceptsGA INSENSITIVESeed germinationEmbryo developmentDouble mutantGai-t6GERMINATION 1Mutant seedsGa1-3Germination pathwaySingle mutantsRepressorUnexpected involvementMutantsDistinct rolesGerminationGermination propertiesIndependent branchInteractive effectsGERMINATION1DAG1DormancyGenesSeedsPathwayInactivation